Journal of Conservation Science (보존과학회지)

The Korean Society Of Conservation Science For Cultural Heritage (한국문화재보존과학회)
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Analysis of Human Skeletal Remains of the Joseon Dynasty from Hwamyeong-dong, Busan: A Molecular Genetic Approach

분자유전학적 접근을 통한 조선시대 사람뼈의 분석 - 부산 화명동 조선시대 분묘군 출토 사람뼈를 중심으로 -

  • Kim, Sue Hoon (Conservation Science Division, National Research Institute of Cultural Heritage) ;
  • Cho, Eun Min (Conservation Science Division, National Research Institute of Cultural Heritage) ;
  • Kim, Yun-Ji (Conservation Science Division, National Research Institute of Cultural Heritage) ;
  • Choe, Hyeongoo (Conservation Science Division, National Research Institute of Cultural Heritage) ;
  • Kang, Soyeong (Conservation Science Division, National Research Institute of Cultural Heritage)
  • 김수훈 (국립문화재연구소 보존과학연구실) ;
  • 조은민 (국립문화재연구소 보존과학연구실) ;
  • 김윤지 (국립문화재연구소 보존과학연구실) ;
  • 최현구 (국립문화재연구소 보존과학연구실) ;
  • 강소영 (국립문화재연구소 보존과학연구실)
  • Received : 2016.10.11
  • Accepted : 2018.01.25
  • Published : 2018.02.20
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Abstract

The analysis of ancient DNA extracted from archaeological bones has become an important research tool in palaeogenetics and anthropology. Eight human skeletal remains of the Joseon dynasty, excavated from Hwamyeong-dong, were used in this study. DNA was extracted from bone powder using a silica-based protocol. The isolated DNA was analyzed by the sequencing variation of hyper-variable region of the mitochondrial DNA. In the present study, 3 human remains were identified into mtDNA haplogroups including the A 5a, D4a, and M4"67+16311 groups, using HaploGrep 2 program. The identified haplotypes of the 3 samples have been confirmed that the specimens in the tombs were not related by the maternal line. This is the first analysis of human skeletal remains of the Joseon dynasty excavated in Busan. Date from the analysis of human remains from the Joseon dynasty are considered as the basis for understanding the genetic relationship between modern and ancient humans of the Korean peninsula.

본 연구는 부산 북구 화명동 조선시대 분묘군에서 출토된 사람뼈를 대상으로 분자유전학적 분석을 수행한 결과이다. 실리카 추출법을 사용하여 토광묘에서 출토된 사람뼈 8개체의 DNA를 분리하였고, 미토콘드리아 DNA 과변이부위 분석을 통해 모계 유연관계 여부를 확인하였다. 분석 결과 토광묘 11호, 21호, 26호에서 출토된 피장자 3명의 하플로타입이 동정되었으며 HaploGrep 2 프로그램에서 A5a, D4a와 M4"67+16311 하플로그룹으로 분류되었다. 하플로그룹이 동정된 3개체는 같은 변이형을 공유하지 않으므로 피장자 간 모계 친연관계는 없는 것으로 확인되었다. 이번 연구는 영남지역에서 출토된 조선시대 사람뼈의 분자유전학적 분석의 첫 사례로서 과거 한반도에 살았던 옛사람들과 현대인들의 유전학적 관계를 이해하기 위한 기초 자료로 활용할 수 있을 것으로 기대된다.

Keywords

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