문화기술의 융합 (The Journal of the Convergence on Culture Technology)

  • 제4권4호
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  • Pages.167-171
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  • 2018
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  • 2384-0358(pISSN)
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  • 2384-0366(eISSN)
국제문화기술진흥원 (The International Promotion Agency of Culture Technology)
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호르몬 및 배지 고형제를 이용한 알스트로메리아 식물체 대량증식 체계 확립

Establishment of propagation system for Alstroemeria plants by using hormones and gelling agents

  • 투고 : 2018.08.15
  • 심사 : 2018.10.12
  • 발행 : 2018.11.30
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초록

알스트로메리아는 경제적으로 중요한 절화 작물 중 하나이다. 그러나 낮은 증식률과 번식기간이 길고 바이러스 질병 감염이 높기 때문에 대량증식이 어렵다고 알려져 있다. 따라서 기내 조직배양을 이용한 대량증식 시스템 쳬계 확립이 필요하다. 본 연구에서는 BA와 NAA 그리고 여러 가지 배지 고형제를 이용하여 알스트로메리아 최적의 생장 효율을 찾기 위하여 실험을 진행하였다. 먼저 BA 1.0 mg/L 와 NAA 0.1 mg/L를 첨가한 배지에서 무처리구에 비하여 신초 및 뿌리의 개수가 약 1.5배 증가하였고 비대한 신초와 뿌리를 나타내었다. 여러 가지 배지 고형제들 중 gelrite 0.25%를 첨가한 배지에서 타 처리구에 비해 최대 50% 향상된 길이를 나타내었고, 근경 개수 및 생체중 증가량에 있어서도 가장 높은 효율을 나타내었다. 본 연구결과를 토대로 알스트로메리아 신품종 개발 및 대량증식 시스템 체계 확립에 도움을 줄 것으로 기대된다.

Alstroemeria (Alstroemeriaceae) is one of the most important cut flowers in international market. But, its characteristics such as low multiplication rates, time consuming process and high risk of carrying viral disease, in vitro propagation techniques based on rhizome meristems culture have been developed. In this study, we conducted this experiment to investigate the effect of hormones and gelling agents on the growth for Alstroemeria in vitro tissue culture. The highest number of shoot and root formation were obtained from the growth medium contains BA 1.0 mg/L and NAA 0.1 mg/L. When 0.25% of gelrite was added up to 50% shoots and roots length were observed compared to other gelling agents. Using these results, it is expected to contribute to the establishment of mass production systems in Alstroemeria.

키워드

GJMGCK_2018_v4n4_167_f0001.png 이미지

그림 1. 호르몬 농도에 따른 알스트로메리아 생육 상태 비교 Figure 1. Conditions of growth in Alstroemeria with concentrations of growth regulators. (A: BA 0.0 + NAA 0.0 B: BA 0.5 + NAA 0.1 C: BA 0.5 + NAA 0.2 D: BA 1.0 + NAA 0.1 E: BA 1.0 + NAA 0.2)

표 1. NAA 와 BA 농도에 따른 알스트로메리아 생육 효율 비교 Table 1. Comparison of the effect of NAA and BA concentrations on length of root and shoot, rhizome formation and fresh weight of Alstroemeria

GJMGCK_2018_v4n4_167_t0001.png 이미지

표 2. 여러 가지 고형제에 따른 알스트로메리아 생육 효율 비교 Table 2. Comparison of the effect of gelling agenets on growth medium for length of root and shoot, rhizome formation and fresh weight of Alstroemeria

GJMGCK_2018_v4n4_167_t0002.png 이미지

참고문헌

  1. Kim G M, "A study on product image analysis and design expression using flowers", The Journal of the Convergence on Culture Technoology, Vol.4(1), pp.231-236, 2018.
  2. Bridgen MP, "Alstroemeria", The Ball Red Book. 16:341-348, 1997.
  3. Aker S, Healy W, "The phytogeography of the genus Alstroemeria", Herbertia (USA) 46:76-87, 1990.
  4. Van Schaik CE, Posthuma A, De Jeu MJ, Jacobsen E, "Plant regeneration through somatic embryogenesis from callus induced on immature embryos of Alstroemeria spp. L", Plant Cell Rep 15:377-380, 1996. https://doi.org/10.1007/BF00232375
  5. Park Sung-Wha, Lee Sang-Hyun, Kim Jong Bo, Jung Hyo Jin, Wi Seong-Gon, and Han Tae-Ho, "Development of Mass Propagation System via Rhizome Culture in Elite Breeding Line C269 of Alstroemeria", Flower Res. J. 25(4) : 232-239, 2017. https://doi.org/10.11623/frj.2017.25.4.07
  6. Murashige T, Skoog F, "Revised medium for rapid growth and bioassays with tobacco tissue culture", Physiol Plant 15:473-497, 1962. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  7. Khaleghi A, Khalighi A, Azadi P, Mii M, "Induction of embryogenic callus and plant regeneration from nodes of greenhouse grown plants of Alstroemeria cv. Fuego", J Food Agr and Environ 6:374-377, 2008.
  8. Pedraza-Santos ME, Lopez-Peralta MC, Gonzalez-Hernandez VA, Engleman-Clark EM, Sanchez-Garcia P, "In vitro regeneration of Alstroemeria cv. 'Yellow King' by direct organogenesis", Plant cell, tissue and organ culture 84:189-198, 2006. https://doi.org/10.1007/s11240-005-9020-z
  9. Kristiansen K, Ornstrup H, Brandt K, "In vitro PPFD and media composition affect both in and ex vitro performance of Alstroemeria Butterfly-hybrids", Plant Cell Tissue and Organ Culture 56:145-153, 1999. https://doi.org/10.1023/A:1006208119297
  10. Pedersen C, Hansen CW, Brandt K, Kristiansen K, "Alstroemeria plantlets can be induced to flowering by cold treatment during in vitro culture", Scientia Horticulturae 66:217-228, 1996. https://doi.org/10.1016/S0304-4238(96)00917-X
  11. Pierik RLM, Van Voorst A, Booy G, Van Acker CAM, Lelivelt CLC, De Wit JC, "Vegetative propagation of Alstroemeria hybrids in vitro", International Symposium on Propagation of Ornamental Plants 226:81-90, 1998.
  12. Elisabeth Chevreau, Fabienne Mourgues, Martine Neveu, and Michel Chevalier, "Effect of Gelling agents and antibiotics on adventitious bud regeneration from in vitro leaves of pear", In vitro Cell. Dev. Biol-Plant 33:173-179, 1997. https://doi.org/10.1007/s11627-997-0017-7
  13. Lin CC and Casida L.E, "Gelrite as a Gelling agents in media for the growth of thermophilic microorganisms", Applied and Environmental Microbiology. 427-429, 1984.