우슬(牛膝)의 증숙(蒸熟)에 따른 항산화 효능 및 고지혈증 유발 흰쥐에 대한 고지혈증 개선 효능에 대한 연구

Studies on Antioxidant and Anti-hyperlipidemic Effects in Induced Hypercholesterolemic Rats of Steamed Achyranthis Bidentatae Radix

Abstract

Objectives : This study was conducted to compare the effect of Achyranthis Bidentatae Radix on antioxidant changes and antihyperlipidemics in hypercholesterolemic rats according to changes in the number of steams. Methods : The experimental animals were divided into six groups: normal (Nor), high cholesterol diet (Veh), high cholesterol diet with Simvastatin 5 mg/kg (Sim), high cholesterol diet with AR0 extract 200 mg/kg (AR0), high cholesterol diet with AR6 extract 200 mg/kg (AR6) and high cholesterol diet plus AR9 extract 200 mg/kg (AR9). I measured HDL, LDL TC, TG contents and ROS from each serums and compared them. I analyzed antioxidant-related protein expressions and cholesterol-related protein expressions in each liver tissues. I also performed optical microscopic analysis through liver tissue staining. Results : The Antioxidant activity of Achyranthis Bidentatae Radix was increased by steaming processes. In vivo, AR0 decreased liver weight in hyperlipidemic rats. LDL TC, TG, AI and CRF were decreased but HDL was increased in AR0. AST, ALT, creatinine, and BUN were decreased in AR0. ROS was decreased in AR0, AR6, and AR9. SOD, catalase and GPx were decreased by steaming processes. p-AMPK was decreased but SREBP-2, p-ACC, and HMGCR were increased by steaming processes. Histologic analysis showed that AR0 decreased liver fat accumulation. Conclusion : In conclusion, AR0 can be developed as a treatment for hyperlipemia. And it is required to research on antioxidant effect changes among different times steamed Achyranthis Bidentatae Radix.

Fig. 1. Steaming and drying equipments.

Fig. 2. Appearance of Achyranthis Bidentatae Radix by processing-steaming and drying.

Fig. 3. Cross section of Achyranthis Bidentatae Radix by processing-steaming and drying.

Fig. 4. Liver weights of high-cholesterol fed rats

Fig. 5. TC level in rats fed high cholesterol diet

Fig. 6. TG level in rats fed high cholesterol diet

Fig. 7. HDL-cholesterol level in rats fed high cholesterol diet

Fig. 8. LDL-cholesterol level in rats fed high cholesterol diet.

Fig. 9. Evaluation of AI in rats fed high cholesterol

Fig. 10. Evaluation of CRF in rats fed high cholesterol

Fig. 11. Evaluation of AST in rats fed high cholesterol diet for 8 weeks

Fig. 12. Evaluation of ALT in rats fed high cholesterol diet for 8 weeks

Fig. 13. Evaluation of creatinine in rats fed high cholesterol diet for 8 weeks

Fig. 14. Evaluation of BUN in rats fed high cholesterol diet for 8 weeks

Fig. 15. ROS level of serum in rats fed high cholesterol diet

Fig. 16. The effects of on the SOD protein expression in rats fed high cholesterol diet

Fig. 17. The effects of on the catalase protein expression in rats fed high cholesterol diet

Fig. 18. The effects of on the GPx protein expression in rats fed high cholesterol diet

Fig. 19. The effects of on the p-AMPK protein expression in rats fed high cholesterol diet

Fig. 20. The effects of on the SREBP-2 protein expression in rats fed high cholesterol diet

Fig. 21. The effects of on the p -ACC protein expression in rats fed high cholesterol diet

Fig. 23. Liver H&E staining histology (×100)

Fig. 22. The effects of on the HMGCR protein expression in rats fed high cholesterol diet

Fig. 24. Liver oil red O staining histology (×100)

Table. 1 The Ingredients of High Cholesterol Diet

Table 2. Explanation of Initials Used in The Experiments

Table. 3. DPPH and ABTS radical scavenging activity of Achyranthis Bidentatae Radix

Table. 4. Total polyphenol contents and Total flavonoid contents of Achyranthis Bidentatae Radix

Table. 5. Initial Body Weight, Body Weight Gain and Food Intake, Food Efficiency Ratio in SD Rats