The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory effects of Coptidis Rhizoma in chronic cold stress-exposed mice

만성 저온 스트레스 동물모델에서의 황련(黃連)의 항염증 효능 연구

  • Choi, Jin Gyu (Department of Pharmacy, College of Pharmacy, Kyung Hee University) ;
  • Huh, Eugene (Department of Medical Science of Meridian, Graduate School, Kyung Hee University) ;
  • Lee, Wonil (Department of Life and Nanopharmaceutical Sciences, Graduate school, Kyung Hee University) ;
  • Kim, Yun-Kyung (Department of Herbal Medicine, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Lee, Tae-Hee (Department of Formulae Pharmacology, School of Oriental Medicine, Gachon University) ;
  • Oh, Myung Sook (Department of Life and Nanopharmaceutical Sciences, Graduate school, Kyung Hee University)
  • 최진규 (경희대학교 약학대학 약학과) ;
  • 허유진 (경희대학교 일반대학원 경락의과학과) ;
  • 이원일 (경희대학교 일반대학원 나노의약생명과학과) ;
  • 김윤경 (원광대학교 약학대학 한약학과) ;
  • 이태희 (가천대학교 한의과대학 방제학교실) ;
  • 오명숙 (경희대학교 일반대학원 나노의약생명과학과)
  • Received : 2018.08.08
  • Accepted : 2018.11.25
  • Published : 2018.11.30
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Abstract

Objectives : The aim of this study was to investigate whether the extract of Coptidis Rhizoma inhibits inflammation in chronic cold stress (CCS)-exposed mice or not. Methods : Coptidis Rhizoma extract (CRE) was made by reflux with distilled water. Male ICR mice (7 weeks old) were divided randomly into 5 groups: (1) control, (2) CCS, (3) CCS+CRE 100 mg/kg, (4) CCS+CRE 300 mg/kg, (5) CCS+CRE 1,000 mg/kg groups. Mice were orally administered once a day for 14 days starting from 1 day before CCS. Group (2)-(5) were exposed to CCS conditions that maintained at $4^{\circ}C$ for 2 h once a day for 14 days. The levels of serum cortisol and hypothalamic prostaglandin E1 (PGE1) and PGE2 were measured by enzyme-linked immunosorbent assay kit. The expression levels of several pro-inflammatory factors like heat shock protein 70 (HSP70), c-fos, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) were measured by western blot analysis in mouse hypothalamus. Results : Oral administration of CRE 1,000 mg/kg significantly suppressed the increase of serum cortisol levels in mice exposed to CCS. CCS-exposed mice had significantly increased the expression of HSP70, c-fos, and NF-kB in hypothalamus, while CRE treatment significantly attenuated the elevation of these pro-inflammatory factors. The ratio of PGE2/PGE1 was also higher in CCS-exposed mice than control group. CRE treatment significantly reduced the increase of PGE2/PGE1 ratio induced by CCS. Conclusion : These findings suggest that Coptidis Rhizoma may work as a potential agent to modulate inflammatory responses under the condition of cold adaptation formed by CCS.

Keywords

DHBCBU_2018_v33n6_35_f0001.tif 이미지

Fig 1. Effects of CRE on cortisol level in mouse serum after CCS.

DHBCBU_2018_v33n6_35_f0002.tif 이미지

Fig 2. Effects of CRE on the expressions of HSP70, c-fos, and NF-kB in mouse hypothalamus after CCS.

DHBCBU_2018_v33n6_35_f0003.tif 이미지

Fig 3. Effects of CRE on the ratio of PGE2/PGE1 levels in mouse hypothalamus after CCS.

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