The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-oxidant effect of ethanol extract from mixture including Caryophylli Flos, Aucklandiae Radix and Angelicae Dahuricae Radix

정향 및 목향과 백지를 혼합한 한약재 에탄올 추출물의 항산화 효능

  • Youn, Seok Na (Department of Public Health Graduate School, Daegu Haany University) ;
  • Kim, Yoo Jin (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Yoo, Wang Keun (Department of Health Science, Daegu Haany University)
  • 윤석나 (대구한의대학교 일반대학원 보건학과) ;
  • 김유진 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 유왕근 (대구한의대학교 보건학부)
  • Received : 2018.10.11
  • Accepted : 2018.11.25
  • Published : 2018.11.30
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Abstract

Objectives : Herbal medicinal mixture (JMB) are consisted of Caryophylli Flos, Aucklandiae Radix and Angelicae Dahuricae Radix. Each of herbal medicines has studied on anti-oxidant effect. So this study was conducted to investigate efficacy and potency of JMB on anti-oxidation. Methods : The JMB was extracted at room temperature by 80% ethanol. And total polyphenol contents, total flavonoid contents in JMB ethanol extract were determined by colorimetric method. Also, DPPH, ABTS free radical scavenging capacity and reducing power of JMB ethanol extract were measured at 100, 500, 1000, $5000{\mu}g/m{\ell}$ concentrations by spectrometric assay. Positive control was used BHA (butylated hydroxyanisole). Results : The total polyphenol contents and total flavonoid contents of the extract were 55.38 mg/TAEg, 513.72 mg/RUEg, respectively. Also, DPPH free radical scavenging capacity and reducing power of JMB ethanol extract in treated concentrations (100, 500, 1000, $5000{\mu}g/m{\ell}$) increased dose dependently. In particular, DPPH free radical scavenging capacity of JMB ethanol extract at 500, 1000, $5000{\mu}g/m{\ell}$ was similar to positive control (BHA) at high concentration (50, $100{\mu}g/m{\ell}$). ABTS free radical scavenging capacity of JMB ethanol extract at 500, 1000, 5000 ug/ml was similar to BHA at high concentration (50, $100{\mu}g/m{\ell}$). Also, reducing power was showed that JMB ethanol extract at $5000{\mu}g/m{\ell}$ was similar to BHA at high concentration (50, $100{\mu}g/m{\ell}$). Conclusions : These results suggest that JMB ethanol extract has effects to scavenge free radicals. Therefore, JMB has potential and applicable benefits for development of materials and products to have anti-oxidation functions.

Keywords

DHBCBU_2018_v33n6_1_f0001.tif 이미지

Figure 1. DPPH free radical scavenging activities of ethanol extract from mixed herbal medicine (JMB).

DHBCBU_2018_v33n6_1_f0002.tif 이미지

Figure 2. ABTS radical scavenging activities of ethanol extract from mixed herbal medicine (JMB).

DHBCBU_2018_v33n6_1_f0003.tif 이미지

Figure 3. Reducing power of ethanol extract from mixed herbal medicine (JMB).

Table 1. Composition of mixed herbal medicine (JMB) ethanol extract

DHBCBU_2018_v33n6_1_t0001.tif 이미지

Table 2. The total polyphenol and total flavonoid contents of ethanol extract from mixed herbal medicine (JMB).

DHBCBU_2018_v33n6_1_t0002.tif 이미지

References

  1. Textbook compilation committee of herbal pharmacology. Herbal pharmacology. Shinilbooks. 2006 ; 2 : 608-610.
  2. Professor committee of herbal medicine in Korea. Herbal medicine. The Korean pharmaceutical association. Seoul. 1995 ; 409-411.
  3. Park SI. Inhibition effect of clove extract on the thermal oxidation of commercial edible oils. Hankyong national university master's degree. 2004.
  4. Dong S. Physiological activities of clove by the different extraction solvents Hankyong national University master's degree. 2004.
  5. Heo J. Donguibogam. Bubinbooks. 2005 ; 1 : 3588.
  6. Chaieb K, Zmantar T, Ksouri R, Hajlaoui H, Mahdouani K, Abdelly C, Bakhrouf A. Antioxidant properties of the essential oil of Eugenia caryophyllata and its antifungal activity against a large number of clinical candida species. Mycoses. 2007 ; 50(5) : 403-406. https://doi.org/10.1111/j.1439-0507.2007.01391.x
  7. Hollman PCH, Katan MB. Absorption, metabolism and health effects of dietary in man. Biomed & Pharmacother. 1997 ; 51 : 305-310. https://doi.org/10.1016/S0753-3322(97)88045-6
  8. Prasad NS, Raghavendra R, Lokesh BR, Naidu KA. Spice phenolics inhibit human PMNL 5-lipoxygenase. Prostaglandins leukot essent fatty acids. 2004 ; 70 : 521-528. https://doi.org/10.1016/j.plefa.2003.11.006
  9. Mytle N, Anderson GL, Doyle MP, Smith MA. Antimicrobial activity of clove (Syzgium aromaticum) oil in inhibiting Listeria monocytogenes on chicken frankfurters. Food Control. 2006 ; 17 : 102-107. https://doi.org/10.1016/j.foodcont.2004.09.008
  10. Gowda NKS, Malathi V, Suganthi RU. Effect of some chemical and herbal compounds on growth of Aspergillus parasiticus and aflatoxin production. Anim Feed Sci Technol. 2004 ; 116 : 281-291. https://doi.org/10.1016/j.anifeedsci.2004.02.008
  11. Kim SI, Yi JH, Tak JH, Ahn YJ. Acaricidal activity of plant essential oils against Dermanyssus gallinae (Acari: Dermanyssidae). Vet Parasitol. 2004 ; 120 : 297-304. https://doi.org/10.1016/j.vetpar.2003.12.016
  12. Jeon YJ, Lee HS, Yeon SY, Ko JH, An KM, Yu SW, Kang JH, Hwang BY, Kin TY. Inhibitory effect of dehydrocostuslactone isolated from Sussureae Radix on CDK2 activity. Kor J Pharmacogn. 2005 ; 36 : 97-101.
  13. The textbook compilation committee of herbalogy of oriental medical schools in nation. Herbalogy. Seoul : Younglim Publisher. 2011 : 395-396.
  14. Jung NH, Ha JY, Min KR, Shibata F, Nakagawa H, Kang SS, Chang IM, Kim YS. Reynosis from Saussurea lappa as inhibitor on CINC-1 induction in LPS-stimulated NRK-52E cells. Planta Med. 1998 ; 64 : 454-456. https://doi.org/10.1055/s-2006-957480
  15. Kang SH, Kim JS, Chi HJ, Chang SY, Ha KW. Isolation and quantitative determination of costunolide from saussurea root. Kor J Pharmacogn. 1999 ; 30 : 48-53.
  16. Wedge DE, Galindo JC, Macias FA. Fungcidal activity of natural and synthetic sesquiterpene lactone analogs. Phytochem. 2000 ; 53: 747-757. https://doi.org/10.1016/S0031-9422(00)00008-X
  17. Park HJ, Jung WT, Basnet P, Kadota S, Namba T. Syringin 4-O-beta glucoside, a new phenylpropanoid glycoside, and costunolide, a nitric oxide synthase inhibitor, from the stem bark of Magnolia sieboldii. J Nat Prod. 1996 ; 59: 1128-1130. https://doi.org/10.1021/np960452i
  18. Jeong SJ, Ltokawa T, Shibuya M, Kuwano M, Ono H, Huguchi R, Miyamoto T. Costunolide, a sesquiterpene lactone from Saussurea lappa, inhibits the VEGFR KDR/Flk-1 signaling pathway. Cancer Lett. 2002 ; 187 : 129-133. https://doi.org/10.1016/S0304-3835(02)00361-0
  19. Lemberkovics E, Czinner E, Szentmihalyi K, Balazs A, Szoke E. Comparative evaluation of Helichrysi flos herbal extracts as dietary source of plant polyphenols, and macro- and microelements. Food Chem. 2002 ; 78 : 119-127. https://doi.org/10.1016/S0308-8146(02)00204-2
  20. Kim HS, Choi HJ. Studies on essential oils of plants of Angelica genus in Korea (III). J Kor Pharmacogn. 1990 ; 21 : 121-125.
  21. Goo BH. Donguibogam. Seoul : Daejungseogwan. 1994.
  22. Kimura T, But PPH, Guo JX, Sung CK. International collation of traditional and folk medicine: Part 1. World scientific, Singapore. 1996 ; 117-118.
  23. Joo EY, Kang WJ. Analysis on the components of the Angelica dahurica root. J Kor Food Preserv. 2005 ; 12 : 476-481.
  24. Kim SH, Kang SS, Kim CM. Coumarin glycosides from the roots of Angelica dahurica. Arch Pharm Res. 1992 ; 15 : 73-77. https://doi.org/10.1007/BF02973988
  25. Kwon YS, Kim CM. Coumarin glycosides from the roots of Angelica dahurica. J Kor Pharmacogn. 1992 ; 24 : 221-224.
  26. Baek NI, Ahn EM, Kim HY, Park YD. Furanocoumarins from the root of Angelica dahurica. Arch Charm Res. 2000 ; 23 : 467-470. https://doi.org/10.1007/BF02976574
  27. Ryu SY, Kim JC, Kim YS, Kim HT, Kim WK, Choi GJ, Kim JS, Lee SW, Heor JH, Cho KY. Antifungal activities of coumarins isolated from Angelica gigas and Angelica dahurica against plant pathogenic fungi. J Kor Pesticide Science. 2001 ; 5 : 26-35.
  28. Wang NH, Yoshiazaki K, Baba K. Seven new bifuranocoumarins, dahuribirin A-G, from Japanese Bai Zh. Chem Pharm Bull. 2001 ; 49 : 1085-1088. https://doi.org/10.1248/cpb.49.1085
  29. Jin MH, Jung MH, Lim YH, Lee SH, Kang SJ, Cho WG. Promoting synthesis of collagen from Angelica dahurica root. J Kor Pharmacogn. 2004 ; 35 : 315-319.
  30. Kim CM, Kwon YS, Choi SY. Antithrombotic effect of the BuOH soluble fraction of Angelica dahurica root. J Kor Pharmacogn. 1995 ; 26 : 74-77.
  31. Shin KH, Kim ON, Woo WS. Effect of the constituents of Angelica dahurica Radix on hepatic drug metabolizing enzyme activity. J Kor Pharmacogn. 1988 ; 19 : 19-27.
  32. Kim DK, Lim JP, Yang JH, Eom DO, Eun JS, Leem KH. Acetylcholinesterase inhibitors from the roots of Angelica dahurica. Arch Pharm Res 2002 ; 25 : 856-859. https://doi.org/10.1007/BF02977004
  33. Pae HO, Oh HC, Yun YG, Oh GS, Jang SI, Hwang KM, Kwon TO, Lee HS, Chung HT. Imperatorin, a furanocoumarin from Angelica dahurica (Umbelliferae), induces cytochrome c-dependent apoptosis in human promyelocytic leukaemia, HL-60 cells. Pharmacol Toxicol. 2002 ; 91 : 40-48. https://doi.org/10.1034/j.1600-0773.2002.910107.x
  34. Lee YS, Jang SM, Kim NW. Antioxidative activity and physiological function of the Angelica dahurica roots. J Kor Soc Food Sci Nutr. 2007 ; 36 : 20-26. https://doi.org/10.3746/jkfn.2007.36.1.020
  35. Choi JI, Kim YJ, Kim JH, Song BS, Yoon Y, Byun MW, Kwon JH, Chun SS, U Lee JW. Antioxidant activities of the extract fractions from Suaeda japonica. J Kor Soc Food Sci Nutr. 2009 ; 38 : 131-135. https://doi.org/10.3746/jkfn.2009.38.2.131
  36. Shin SR, Hong JY, Nam HS, Yoon KY, Kim KS. Anti-oxidative effects of extracts of Korean herbal materials. J Kor Soc Food Sci Nutr. 2006 ; 35 : 187-191. https://doi.org/10.3746/jkfn.2006.35.2.187
  37. Jeon YH, Kil JH, Lim SM, Kim MH, Kim MR. Analysis of antioxidative activity and antimutagenic effect of ethanol extract from Schizandra chinensis Baillon. J East Asian Soc Diet Life. 2008 ; 18: 746-752.
  38. Kwon TH, Kim JK, Kim TW, Lee JW, Kim JT, Seo HJ, Kim MJ, Kim CG, Jeon DS, Park NH. Antioxidant and anti-lipase activity in Halocynthia roretzi extracts. J Kor Food Sci Technol. 2011 ; 43 : 464-468. https://doi.org/10.9721/KJFST.2011.43.4.464
  39. Park BH, Yang HH, CHo HS. Quality characteristics and antioxidative effect of Yukwa prepared with Lycii Fructus powder. J Kor Soc Food Sci Nutr. 2012 ; 41 : 745-751. https://doi.org/10.3746/jkfn.2012.41.6.745
  40. Kim YJ, Park HJ, Lee JS, Do EJ, Sohn HR, Jeon SM, Yeum JH, Kim MR. Antioxidant effect of ethanol extract from Poria cocos depending on cultivation methods. Kor J Herbol. 2016 ; 31(5) : 107-114. https://doi.org/10.6116/kjh.2016.31.5.107.
  41. Kim JY, Park HJ, Kim MR. Antioxidant activities of ethanol extract of Shinsun-yukza-hwan, a Korean medicinal recipe. Kor J Herbol. 2017 ; 32(5) : 19-25. https://doi.org/10.6116/KJH.2017.32.5.19
  42. Kim YJ, Kim SY, Jeong MJ, Lee UT, Choo ST, Youn SN, Kim MR. Antioxidant effect of ethanol extract from Plantaginis Herba. Kor J Herbol. 2018 ; 33(3) : 37-43. https://doi.org/10.6116/KJH.2018.33.3.37
  43. Jang YS, Jeong JM. Effects of phyto-extract mixture on adiposity and serum lipid levels in obese mice induced by high fat diet. J Kor Soc Food Sci Nutr. 2010 ; 39 : 1439-1445. https://doi.org/10.3746/jkfn.2010.39.10.1439
  44. Ku JI, Kong MR, Lee YS. Anti-aging and antioxidant activity of ultrasonification ethanolic extract from Portulaca oleracea. J Invest Cosmetol, 2015 ; 11(2) : 97-106. https://doi.org/10.15810/jic.2015.11.2.002
  45. Kim JW, Kim SD, Youn KS. Antioxidant activity of Hwangki and Beni-Koji extracts and mixture. J Kor Soc Food Sci Nutr. 2011 ; 40 : 1-6. https://doi.org/10.3746/jkfn.2011.40.1.001
  46. Williams GM, Iatropoulos MJ, Whysner J. Safety assessment of butylated hydroxyanisole and butylated hydroxytoluene as antioxidant food additives. Food Chem Toxicol. 1999 ; 37(9) : 1027-1038. https://doi.org/10.1016/S0278-6915(99)00085-X
  47. Urquiaga I, Leighton F. Plant polyphenol antioxidants and oxidative stress. Biol Res. 2000 ; 33 : 55-64.
  48. Dai J, Mumper RJ. Plant phenolics: Extraction, analysis, and their antioxidant and anticancer properties. Molecules. 2010 ; 15 : 7313-7352. https://doi.org/10.3390/molecules15107313
  49. Lu Y, Foo LY. Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem. 2000 ; 68 : 81-85. https://doi.org/10.1016/S0308-8146(99)00167-3
  50. Cha JY, Kim HJ, Chung CH, Cho YS. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J Kor Soc Food Sci Nutr. 1999 ; 28 : 1310-1315.
  51. Hetog MGL, Hollman PCH, Van de Putte B. Content of potentially anticarcinogenic flavonoids of tea infusions, wines and fruit juice. J Agr Food Chem. 1993 ; 41 : 1242-1246. https://doi.org/10.1021/jf00032a015
  52. Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients. 2010 ; 2 : 1231-1246. https://doi.org/10.3390/nu2121231
  53. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: Chemistry, metabolism, and structure-activity relationships. J Nutr Biochem. 2002 ; 13 : 572-584. https://doi.org/10.1016/S0955-2863(02)00208-5
  54. Williams RJ, Spencer JP, Rice-Evans C. Flavonoids: Antioxidants or signaling molecules? Free Radic Bio Med. 2004 ; 36 : 838-849. https://doi.org/10.1016/j.freeradbiomed.2004.01.001
  55. Sohn HY, Ryu HY, Jang YJ, Jang HS, Park YM, Kim SY. Evaluation of antimicrobial, antithrombin, and antioxidant activity of aerial part of Saxifraga stolonifera. Kor J Microbiol Biotechnol. 2008 ; 36 : 195-200.
  56. Kim EJ, Choi JY, Yu MR, Kim MY, Lee SH, Lee BH. Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Kor J Food Sci Technol. 2012 ; 44(3) : 337-342. https://doi.org/10.9721/KJFST.2012.44.3.337
  57. Cho BY, Lee JH, Choi SI, Jung TD, Choi SH, Ra MJ, Kim SY, Kang IJ, Han KC, Lee OH. Changes in antioxidant and antiobesity activities of Cirsium setidens Nakai ethanolic extract depending on different harvest time. J Food Hyg Saf. 2017 ; 32 : 234-242. https://doi.org/10.13103/JFHS.2017.32.3.234
  58. Rice-Evans CA, Miller NJ, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci. 1997 ; 2 : 152-159. https://doi.org/10.1016/S1360-1385(97)01018-2
  59. Kim JH, Kim JK, Kang WW, Ha YS, Choi SW, Moon KD. Chemical composition and DPPH radical scavenger activity in different sections of safflower. J Kor Soc Food Sci Nutr. 2003 ; 32 : 733-738. https://doi.org/10.3746/jkfn.2003.32.5.733
  60. Cha JY, Yang HJ, Jeong JJ, Seo WS, Park JS, Ok M, Cho YS. Tyrosinase inhibition activity and antioxidant capacity by fermented products of some medicinal plants. Journal of life science. 2010 ; 20(6) : 940-947. https://doi.org/10.5352/JLS.2010.20.6.940
  61. Lee HJ, Lim MH. Biochemical antioxidant activity and inhibitory effect of extract of Aucklandia lappa Decne against cell differentiation in 3T3-L1 adipocytes. J Kor Soc Cosm. 2018 ; 24(1) : 76-84.
  62. Lee YS, Jang SM, Kim NW. Antioxidative activity and physiological function of the Angelica dahurica roots. J Kor Soc Food Sci Nutr. 2007 ; 36(1) : 20-26. https://doi.org/10.3746/jkfn.2007.36.1.020