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Chemical Composition and Bioactivity of Korean Green Tea (Camellia sinensis) Pollen collected by Honey Bee

한국 녹차 화분의 화학적 조성과 기능성 연구

  • Received : 2017.09.18
  • Accepted : 2018.01.11
  • Published : 2018.02.28

Abstract

In this study, we investigated the nutritional composition including proximate, amino acid, vitamin, minerals, and the antioxidant activity of green tea (Camellia sinensis) pollen grains collected by Apis mellifera bees, for use as a health food. The crude protein and fat content was estimated at 26.14% and 3.49%, respectively. Eighteen amino acids were identified in green tea pollen, including 8 essential amino acids and 10 non-essential amino acids. The predominant amino acids were glutamic acid, proline and aspartic acid accounting for about 33.3% of total free amino acids. The concentration of vitamin C was the highest value of 35.7%, followed by $B_3$ and $B_2$ among the detected vitamins. The predominant minerals were potassium (790.32 mg/100g), followed by phosphorus (707.52 mg/100g) and sulfur (302.67 mg/100g), whereas copper, zinc and sodium were detected as minor elements. The antioxidant activity and phenolic content accounted for 33.8% at $500{\mu}g/mL$ extract and $2.55 {\mu}g/mg$, respectively.

Keywords

References

  1. AOAC. 2010. The Association Official Methods of Analysis. pp. 33-36
  2. Bae MS. 2016. Current status and tasks of green tea industry. national assembly research service. Seoul. Korea
  3. Beretta G, Granata P, Ferrero M, Orioli M, Facino RM. 2005. Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Analytica Chimica Acta 533:185-191
  4. Chung YG, Yoon SH, Kwon JS, Bae MJ. 1984. Nutritional and biochemical studies on the pollen loads studies on lipid compositions of sunflower pollen load and effects of its pollen load on liver cholesterol metabolism in mouse. J Korean Soc Food Nutr 13:169-174
  5. Choi SJ, Jeong YH. 2004. Effect of proteases on the extraction of crude protein and reducing sugar in pollen. J Korean Soc Food Sci Nutr 33:1353-1358
  6. Han MR, Lee SJ, Kim MH. 2004. Development of pine pollen cell wall rupture technique using a high impact planetary milling process. Dankook Journal of the New Material Technology 12:43-54
  7. Hong IP, Lee MY, Woo SO, Sim HS, Choi YS, Han SM, Kim HK, Byeon KH, Lee ML, Ha NG. 2013a. Change of chemical composition in acorn pollen after physical treatment. Korean J Apiculture 28:217-221
  8. Hong IP, Lee MY, Woo SO, Sim HS, Choi YS, Han SM, Kim HK, Byeon KH, Lee ML, Kim JB. 2013b. The morphological characteristics and fatty acids composition of pollens in acorn and darae (Actinidia arguta). J Seric Entomol Sci 51:119-122
  9. Hong IP, Woo SO, Han SM, Kim SG, Jang HR, Lee MY, Choi YS, Kim HK, Lee ML. 2016a. Evaluation of nutritional potential of Amorpha fruticosa pollen collected by honey bees. Korean J Apiculture 31:73-77
  10. Hong IP, Woo SO, Han SM, Kim SG, Jang HR, Lee MY, Choi YS, Kim HK, Lee ML. 2016b. Evaluation of nutritional potential and antioxidant activity of Oryza sativa (rice) pollen collected by honey bee, Apis mellifera. Korean J Apiculture 31:219-225
  11. Kim JG, Son JH. 1990. Progress of chemical composition on pulverization of pollen load. Korean J Apiculture 5:23-30
  12. Kim JK 1986. Chemical composition of pollen load and contents of amino acids. Korean J Apiculture 1:91-96
  13. Kim JW, Shin SC, Kim BK. 1984. Studies on pollen preparations and as a health food(I). Kor J Pharmacogn 15:147- 149
  14. Korea Food & Drug Administration(KFDA). 2016. Food Standard Code. Osong, Korea
  15. LeBlanc BW, Davis OK, Boue S, Delucca A, Deeby T. 2009. Antioxidant activity of Sonoran Desert bee pollen. Food Chem 115:1299-1305
  16. Lee BY, Choi HD, and Hwang JB. 1997. Component analysis of Korean pollens and pollen extracts. Korean J Food Sci Technol 29:869-875
  17. Lee NH, Hong JI, Kim JY, Chiang MH. 2009. Antioxidant properties and protective effects of Inula britannica var. chinensis Regel on oxidative stress-induced neuronal cell damage. Korean J Food Sci Technol 41:87-92
  18. Lew YS. 1988. A review on the efficacy of natural pollen described in an orient medical handbook "Dong-Eui-Pogram". Korean J Apiculture 3:26-47
  19. Liangli Y, Scot A, Jonathan P, Mary H, John W, Ming Q. 2002. Antioxidant properties of hard winter wheat extracts. J Agric Food Chem 60:1619-1624
  20. Ryu JB. 2003. Classification of honey plants in Korea. Korean J Apiculture 18:5-22
  21. Solberg Y, Remedios G. 1980. Chemical composition of pure and bee collected pollen, Meld. Norg Landbruk 59:1-12
  22. Stanley RG, Linskens HF. 1974. Pollen Biology, Chemistry and Management, Springer Verlag, New York
  23. Todd FE, Bretherick O. 1942. The composition of pollens. J Econ Entomol 35:312-317
  24. Wesh SO, Marston RM. 1983. Nutritional Bioavailability of Zin. Washington, D.C.: American Chemical Society
  25. Yoon SH, Ahn JI, Kwon JS. 1985. Nutritional ad biochemical studies on the pollen loads. Amino acid composition of sunflower pollen load and its effects on the hepatic alcohol dehydrogenase (ADH) activity I rat. J Korean Soc Food Nutr 14:27-32
  26. Zhang W, Xu YC, Guo FJ, Meng Y, Li ML. 2008. Antidiabetic effects of cinnamaldehyde and berberine and their impacts on retinol-binding protein 4 expression in rats with type 2 diabetes mellitus. Chin Med J (Engl) 121:2124-2128