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명월초, 여주 및 울금을 포함한 돼지감자 복합물의 항산화 및 AMPK 활성화를 통한 항당뇨 활성

Antioxidant and Antidiabetic Activities of Jerusalem Artichoke Composites Containing Gynura procumbens, Momordica charantia, and Curcuma longa via AMPK Activation

  • 이수정 (경상대학교 농업생명과학연구원) ;
  • 호문사 (경상대학교 식품영양학과) ;
  • 표재호 (산청기능성콩영농조합법인) ;
  • 류지현 (경상대학교 의과대학 생리학교실) ;
  • 강다원 (경상대학교 의과대학 생리학교실) ;
  • 정보영 (경상대학교 농업생명과학연구원) ;
  • 성낙주 (경상대학교 식품영양학과)
  • Lee, Soo-Jung (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Hu, Wen-Si (Department of Food Science and Nutrition, Gyeongsang National University) ;
  • Pyo, Jae-Ho (Sancheong of Functional Bean Farming Union Corporation) ;
  • Ryu, Ji Hyeon (Department of Physiology, College of Medicine, Gyeongsang National University) ;
  • Kang, Dawon (Department of Physiology, College of Medicine, Gyeongsang National University) ;
  • Jeong, Bo-Young (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Sung, Nak-Ju (Department of Food Science and Nutrition, Gyeongsang National University)
  • 투고 : 2017.08.22
  • 심사 : 2017.10.09
  • 발행 : 2018.01.30

초록

명월초, 여주 및 울금의 물 및 70% 에탄올 추출물에서 항산화 및 ${\alpha}$-glucosidase 저해활성을 측정하였으며, 돼지감자 농축액에 이들 식물류의 에탄올 추출물을 첨가한 5종의 돼지감자 복합물(JA1~5)에서 항산화 및 항당뇨 활성을 비교하였다. 명월초, 여주 및 울금의 에탄올 추출물은 물 추출물에 비해 총 페놀 및 플라보노이드 함량이 많았다. DPPH, ABTS 라디칼 소거활성 및 FRAP법에 의한 환원력은 총 페놀 및 플라보노이드 함량에 의존적이었으며, 특히 명월초와 울금의 에탄올 추출물은 항산화 활성이 비슷한 수준이었다. ${\alpha}$-Glucosidase 저해활성은 에탄올 추출물의 $2,000{\mu}g/ml$ 농도에서 50% 이상이었다. 5종의 복합물은 돼지감자 농축액에 비해 총 페놀 및 플라보노이드 함량이 많았다. 또한 항산화 활성 및 ${\alpha}$-glucosidase 저해활성도 복합물에서 모두 증가되었으며, 특히 JA1의 활성이 유의적으로 높았다. 돼지감자 복합물은 인체 정상 간세포주인 Chang세포에 대해 세포독성이 없었으며, Chang세포에서 AMPK 활성화 및 근육세포인 C2C12세포에서 세포 내 당 유입을 유의적으로 증가시켰다. 이로써 명월초, 여주 및 울금의 추출물이 혼합된 돼지감자 복합물은 항산화 및 항당뇨 활성 증대에 효과적인 것으로 판단된다.

This study was performed to identify the antioxidant and ${\alpha}$-glucosidase inhibitory activities of water and 70% ethanol extracts of the three following herbs: G. procumbens, M. charantia, and C. longa. In addition, the antioxidant and antidiabetic activities of five types of Jerusalem artichoke composites (JA1 - 5), which were prepared by adding ethanol extracts of several herbs to Jerusalem artichoke concentrate, were studied and compared. The results showed that the total phenol and flavonoid contents of the ethanol extracts were higher than those of the water extracts. The DPPH and ABTS radical scavenging activities and reducing power depended on the total phenol and flavonoid contents. The antioxidant activities of ethanol extracts from G. procumbens and C. longa were comparable. Moreover, the ${\alpha}$-glucosidase inhibitory activity of the ethanol extracts ($2,000{\mu}g/ml$) from each herb was found to be over 50%. In contrast, the five types of JA composites showed higher total phenol and flavonoid contents than those of JA concentrate. In addition, increased antioxidant and ${\alpha}$-glucosidase inhibitory activities were observed, with that of JA1 being the highest. However, all concentrations ($1{\sim}100{\mu}g/ml$) of JA tested did not affect the cell viability of Chang cells. In addition, JA induced the activation of AMP-activated protein kinase (AMPK) in Chang cells and significantly increased the glucose uptake in C2C12 cells. Therefore, it could be concluded that the JA composites (JA1 - 5) mixed with G. procumbens, M. charantia, and C. longa extracts were effective in increasing the extracts' antioxidant and antidiabetic activities.

키워드

참고문헌

  1. Ahn, M. J., Yuk, H. J., Lee, H. Y., Hwang, C. E., Jeong, Y. S., Hong, S. Y., Kwon, O. K., Kang, S. S., Kim, H. R., Park, D. S. and Cho, K. M. 2015. Effect of the enhanced biological activities and reduced bitter taste of bitter melon (Momordica charantia L.) by roasting. J. Agric. Life Sci. 49, 107-119. https://doi.org/10.14397/jals.2015.49.2.107
  2. Bantle, J. P., Rosett, J. W., Albrigh, A. L., Apovian, C. M., Clark, N. G, Frans, M. J, Hoogwerf, B. J, Lichtensterin, A. H., Davis, E. M., Mooradian, A. D. and Wheeler, M. L. 2000. Nutrition recommendation and principles for people with diabetes mellitus. Diabetes Care 23, 843-846.
  3. Benzie, I. F. F. and Strain, J. J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal. Biochem. 239, 70-76. https://doi.org/10.1006/abio.1996.0292
  4. Blois, M. S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199-1200. https://doi.org/10.1038/1811199a0
  5. Boo, H. O., Lee, H. H., Lee, J. W., Hwang, S. J. and Park, S. U. 2009. Different of total phenolics and flavonoids, radical scavenging activities and nitrite scavenging effects of Momordica charantia L. according to cultivars. Kor. J. Medicinal Crop Sci. 17, 15-20.
  6. Cha, J. H., Jin, J. S. and Cho, Y. S. 2011. Biological activity of methanolic extract from Ganoderma lucidum, Momordica charantia, Fagopyrum tataricum, and their mixtures. J. Life Sci. 21, 1016-1024. https://doi.org/10.5352/JLS.2011.21.7.1016
  7. Cho, H. S., Lee, S. J., Shin, J. H., Kang, M. J., Cho, H. S., Lee, H. J. and Sung, N. J. 2007. Antioxidative activity and nitrite scavenging effect of the composites containing medicinal plant extracts. J. Life Sci. 17, 1135-1140. https://doi.org/10.5352/JLS.2007.17.8.1135
  8. Choi, S. I., Lee, H. A. and Han, J. S. 2016. Gynura procumbens extract improves insulin sensitivity and suppresses hepatic gluconeogenesis in C57BL/KsJ-db/db mice. Nutr. Res. Pract. 10, 507-515. https://doi.org/10.4162/nrp.2016.10.5.507
  9. Choi, S. I., Park, M. H. and Han, J. S. 2016. Gynura procumbens extract alleviates postprandial hyperglycemia in diabetic mice. Prev. Nutr. Food Sci. 21, 181-186. https://doi.org/10.3746/pnf.2016.21.3.181
  10. Gutfinger, T. 1981. Polyphenols in olive oil. J. Am. Oil Chem. Soc. 58, 966-968. https://doi.org/10.1007/BF02659771
  11. Han, J. T., Bang, M. H., Chun, O. K., Kim, D. O., Lee, C. Y. and Baek, N. I. 2004. Flavonol glycosides from the aerial parts of Aceriphyllum rossii and their antioxidant activities. Arch. Pharm. Res. 27, 390-395. https://doi.org/10.1007/BF02980079
  12. Hanefeld, M. 1998. The role of acarbose in the treatment of non-insulin-dependent diabetes mellitus. J. Diabetes Complications 12, 228-237. https://doi.org/10.1016/S1056-8727(97)00123-2
  13. Hu, W. S. 2017. Antioxidants and antidiabetic effects in the composite by Gynura procumbens, Momordica charantia, Curcuma longa and Helianthus tuberosus. MS dissertation, Gyeongsang National University, Jinju, Korea.
  14. Hyun, M. R. 2012. Effects of curcumin on high glucose-induced podocyte injury. MS dissertation, Soonchunhyang University, Asan, Korea.
  15. Jeon, H. J. and Kwon, H. J. 2014. Antioxidant effects and functional evaluation of Gynura procumbens extract as a collaboration material for cosmetics and functional food. Kor. J. Aesthet. Cosmetol. 12, 499-507.
  16. Jeon, H. J., Cha, Y. H. and Jang, H. R. 2016. Isolation and rutin quantitative analysis of antioxidants substances from Gynura procumbens extract. J. Welliness 11, 165-466.
  17. Jeong, H. J., Kim, J. S., Sa, Y. J., Kim, M. O., Yang, J. F. and Kim, M. J. 2011. Antioxidant activity and ${\alpha}$-glucosidase inhibitory effect of Jerusalem artichoke (Helianthus tuberosus) methanol extracts by heat treatment conditions. Kor. J. Medicinal Crop Sci. 19, 257-263. https://doi.org/10.7783/KJMCS.2011.19.4.257
  18. Jeong, H. J., Lee, S. G., Lee, E. J., Park, W. D., Kim, J. B. and Kim, H. J. 2010. Antioxidant activity and anti-hyperglycemic activity of medicinal herbal extracts according to extraction methods. Kor. J. Food Sci. Technol. 42, 571-577.
  19. Jeong, J. H., Lee, S. H., Hue, J. J., Lee, K. N., Nam, S. Y., Yun, Y. W., Jeong, S. W., Lee, Y. H. and Lee, B. J. 2008. Effect of bitter melon (Momordica charantia) on anti-diabetic activity in C57BL/6J db/db mice. Kor. J. Vet. Res. 48, 327-336.
  20. Joseph, B. and Jini, D. 2013. Antidiabetic effects of Momordia charantia (bitter melon) and its medicinal potency. Asian Pac. J. Trop. Dis. 3, 93-102. https://doi.org/10.1016/S2222-1808(13)60052-3
  21. Jung, Y. S., Park, S. J., Park, J. H., Jhee, K. H., Lee, I. S. and Yang, S. A. 2012. Effects of ethanol extracts from Zingiber officinale Rosc., Curcuma longa L., and Curcuma aromatica Salisb. on acetylcholinesterase and antioxidant activities as well as GABA contents. J. Kor. Soc. Food Sci. Nutr. 41, 1395-1401. https://doi.org/10.3746/jkfn.2012.41.10.1395
  22. Kaewseejan, N. and Siriamornpun, S. 2015. Bioactive components and properties of ethanolic extract and its fractions from Gynura procumbens leaves. Ind. Crops Prod. 74, 271-278. https://doi.org/10.1016/j.indcrop.2015.05.019
  23. Kim, C. G., Kim, S. I. and Shin, H. K. 1993. Effect of fructooligosaccharide- inulin of Jerusalem artichoke on the growth of intestinal microorganisms of pig. Kor. J. Food Sci. Technol. 25, 395-399.
  24. Kim, D. J., Kim, J. M., Kim, T. H., Baek, J. M., Kim, H. S. and Choe, M. 2010. Anti-diabetic effects of mixed extracts from Lycium chinense, Cordyceps militaris, and Acanthopanax senticosus. Kor. J. Plant Res. 23, 423-429.
  25. Kim, E. 2008. Anti-diabetic effects of Eriobotrya japonica and Curcuma longa L. in db/db mice. MS dissertation, Mokpo University, Mokpo, Korea.
  26. Kim, H. J., Kim, D. I. and Yon, J. M. 2015. Effects of Jerusalem artichoke (Helianthus tuberosus L.) extracts on blood glucose and lipid metabolism in STZ-induced diabetic rats. Kor. J. Clin. Lab. Sci. 47, 203-208. https://doi.org/10.15324/kjcls.2015.47.4.203
  27. Kim, H. S., Kim, T. W., Kim, D. J., Kim, K. K. and Choe, M. 2013. Effects of medicinal plant water extracts on expression of anti-diabetic enzymes mRNA. J. Kor. Soc. Food Sci. Nutr. 42, 1008-1014. https://doi.org/10.3746/jkfn.2013.42.7.1008
  28. Kim, H. W., Shin, H. J., Hwang, D. B., Lee, J. E., Jeong, H. L. and Kim, D. U. 2015. Functional cosmetic characteristics of Momordica charantia fruit extract. Kor. Chem. Eng. Res. 53, 289-294. https://doi.org/10.9713/kcer.2015.53.3.289
  29. Kim, J. L., Bae, C. R. and Cha, Y. S. 2010. Helianthus tuberosus extract has antidiabetes effects in HIT-T15 cells. J. Kor. Soc. Food Sci. Nutr. 39, 31-35. https://doi.org/10.3746/jkfn.2010.39.1.031
  30. Kim, K. D. 2007. Research of efficacy & stability about mixed medicinal plants extracts. J. Kor. Soc. Cosm. 13, 601-608.
  31. Kim, S. J., Heo, M. Y., Bae, K. H., Kang, S. S. and Kim, H. P. 2003. Tyrosinase inhibitory activity of plant extract (III): Fifty Korean indigenous plants. J. Applied Pharmacol. 11, 245-248.
  32. Kundu, J. K. and Surh, Y. J. 2012. Emerging avenues linking inflammation and cancer. Free Radic. Biol. Med. 52, 2013- 2037. https://doi.org/10.1016/j.freeradbiomed.2012.02.035
  33. Kwon, H. O., Lee, M. H., Kim, Y. J., Kim, E. and Kim, O. K. 2016. Beneficial effects of Acanthopanax senticosus extract in type II diabetes animal model via down-regulation of advanced glycated hemoglobin and glycosylation end products. J. Kor. Soc. Food Sci. Nutr. 45, 929-937. https://doi.org/10.3746/jkfn.2016.45.7.929
  34. Lee, A. J. 2015. Quality characteristics of Jochung containing various level of Helianthus tuberosus L. powder. MS dissertation, Sejong University, Seoul, Korea.
  35. Lee, J. M., Park, J. H., Chu, W. M., Yoon, Y. M., Park, E. J. and Park, H. R. 2011. Antioxidant activity and alpha-glucosidase inhibitory activity of stings of Gleditsia sinensis extracts. J. Life Sci. 21, 62-67. https://doi.org/10.5352/JLS.2011.21.1.62
  36. Lee, M. H., Jo, D. J., Yoon, S. R. and Lee, G. D. 2007. Physicochemical properties of functional herb mixtures. J. Kor. Soc. Food Sci. Nutr. 36, 1571-1577. https://doi.org/10.3746/jkfn.2007.36.12.1571
  37. Lee, S. J., Shin, J. H., Kang, J. R., Hwang, C. R. and Sung, N. J. 2012. In vitro evaluation of biological activities of wa-song (Orostachys japonicus A. Berger) and Korean traditional plants mixture. Kor. Soc. Food Sci. Nutr. 41, 295-301. https://doi.org/10.3746/jkfn.2012.41.3.295
  38. Lo, H. Y., Ho, T. Y., Lin, C. J., Li, C. C. and Hsiang, C. Y. 2013. Momordica charantia and its novel polypeptide regulate glucose homeostasis in mice via binding to insulin receptor. J. Agric. Food Chem. 61, 2461-2468. https://doi.org/10.1021/jf3042402
  39. Moon, J. H., Choi, D. W., Kim, S. E., Seomoon, J. H., Hong, S. Y., Kim, H. K., Cho, K. M., Song, J., Kang, S. S., Kim, K. H. and Kwon, O. K. 2015. Comparison of biological activities of ethanol extracts of unripe fruit of bitter melon (Momordica charantia L.) cultivated in Hamyang, Korea. J. Kor. Soc. Food Sci. Nutr. 44, 1637-1644. https://doi.org/10.3746/jkfn.2015.44.11.1637
  40. Moreno, M. I., Isla, M. I., Sampietro, A. R. and Vattuone, M. A. 2000. Comparison of the free radical-scavenging activity of propolis from several region of Argentina. J. Ethnopharmacol. 71, 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  41. Na, L. X., Zhang, Y. L., Li, Y., Liu, L. Y., Li, R., Kong, T. and Sun, C. H. 2011. Curcumin improves insulin resistance in skeletal muscle of rats. Nutr. Metab. Cardiovasc. Dis. 21, 526-533. https://doi.org/10.1016/j.numecd.2009.11.009
  42. Oh, S. J. and Mo, J. H. 2013. A study on skin related physiological activity of Curcuma longa and fermented Curcuma longa extracts. J. Korea Soc. Beauty Art 14, 185-195.
  43. Park, C. J. and Han, J. S. 2015. Hypoglycemic effect of Jicama (Pachyrhizus erosus) extract on streptozotocin-induced diabetic mice. Prev. Nutr. Food Sci. 20, 88-93. https://doi.org/10.3746/pnf.2015.20.2.88
  44. Park, H. J., Jeon, B. T., Kim, H. C., Roh, G. S., Shin, J. H., Sung, N. J., Han, J. and Kang, D. 2012. Aged red garlic extract reduces lipopolysaccharide‐induced nitric oxide production in raw 264.7 macrophages and acute pulmonary inflammation through haeme oxygenase-1 induction. Acta Physiologica 205, 61-70. https://doi.org/10.1111/j.1748-1716.2012.02425.x
  45. Park, J. J., Lee, J. M. and Jun, W. J. 2013. Radical scavenging and anti-obesity effects of various extracts from turmeric (Curcuma longa L.). J. Kor. Soc. Food Sci. Nutr. 42, 1908-1914. https://doi.org/10.3746/jkfn.2013.42.12.1908
  46. Puangpronpitag, D., Chaichanadee, S., Naowaratwattana, W., Sittiwet, C., Thammasarn, K., Luerang, A. and Kaewseejan, N. 2010. Evaluation of nutritional value and antioxidative properties of the medicinal plant Gynura procumbens extract. Asian J. Plant Sci. 9, 146-151. https://doi.org/10.3923/ajps.2010.146.151
  47. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  48. Rosidah, M., Yam, M. F., Sadikun, A. and Asmawi, M. Z. 2008. Antioxidant potential of Gynura procumbens. Pharm. Biol. 46, 616-625. https://doi.org/10.1080/13880200802179642
  49. Sakamoto, K., Goransson, O., Hardie, D. G. and Alessi, D. R. 2004. Activity of LKB1 and AMPK-related kinases in skeletal muscle: Effects of contraction, phenformin, and AICAR. Am. J. Physiol. Endocrinol. Metab. 287, E310-E317. https://doi.org/10.1152/ajpendo.00074.2004
  50. Sarkar, S., Pranava, M. and Marita, R. 1996. Demonstration of the hypoglycemic action of Momordica charantia in a validated animal model of diabetes. Pharmacol. Res. 33, 1-4. https://doi.org/10.1006/phrs.1996.0001
  51. Shon, M. S. and Kim, G. N. 2014. Anti-oxidant and anti- obese activities of turmeric (Curcuma longa L.) extract in 3T3-L1 cells. Asian J. Beauty Cosmetol. 12, 169-175.
  52. Soetikno, V., Sari, F. R., Sukumaran, V., Lakshmanan, A. P., Harima, M., Suzuki, K., Kawachi, H. and Watanabe, K. 2013. Curcumin decreases renal triglyceride accumulation through AMPK-SREBP signaling pathway in streptozotocin- induced type 1 diabetic rats. J. Nutr. Biochem. 24, 796-802. https://doi.org/10.1016/j.jnutbio.2012.04.013
  53. Son, H. K., Han, J. H. and Lee, J. J. 2014. Anti-diabetic effect of the mixture of mulberry leaf and green tea powder in rats with streptozotocin-induced diabetes. Kor. J. Food Preserv. 21, 549-559. https://doi.org/10.11002/kjfp.2014.21.4.549
  54. Xu, M. L., Wang, L., Hu, J. H. and Wang, M. H. 2009. Antioxidant and ${\alpha}$-glucosidase inhibitory activities of some wild vegetable extracts. J. Agric. Food Chem. 47, 4121-4125.
  55. Yang, H. J., Kwon, D. Y., Kim, M. J., Kang, S., Kim, D. S. and Park, S. 2012. Jerusalem artichoke and chungkookjang additively improve insulin secretion and sensitivity in diabetic rats. Nutr. Metab. 9, 112. https://doi.org/10.1186/1743-7075-9-112
  56. Zhang, B. B., Zhou, G. and Li, C. 2009. AMPK: An emerging drug target for diabetes and the metabolic syndrome. Cell Metab. 9, 407-416. https://doi.org/10.1016/j.cmet.2009.03.012
  57. Zhang, X. F. and Tan, B. K. H. 2000. Effects of an ethanolic extract of Gynura procumbens on serum glucose, cholesterol and triglyceride levels in normal and steptozotocin-induced diabetic rats. Singapore Med. J. 41, 9-13.