Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidative and digestion enzyme inhibitory activity of Ganoderma lucidum depends on the extraction solvent

추출용매에 따른 영지버섯(Ganoderma lucidum)의 항산화 및 소화효소 저해활성

  • Joo, Ok Soo (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Hwang, Chung Eun (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Hong, Su Young (Food Science Research Institute, Kolmar BNH Inc) ;
  • Sin, Eui Cheol (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Nam, Sang Hae (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Cho, Kye Man (Department of Food Science, Gyeongnam National University of Science and Technology)
  • 주옥수 (경남과학기술대학교 식품과학부) ;
  • 황정은 (경남과학기술대학교 식품과학부) ;
  • 홍수영 (콜마비앤에이치(주) 식품과학연구소) ;
  • 신의철 (경남과학기술대학교 식품과학부) ;
  • 남상해 (경남과학기술대학교 식품과학부) ;
  • 조계만 (경남과학기술대학교 식품과학부)
  • Received : 2018.01.31
  • Accepted : 2018.02.20
  • Published : 2018.02.28
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Abstract

This study investigated the nutritional properties and biological activities of Ganoderma lucidum (GL). The round type of GL contained higher carbohydrate content, while the Nokgak type of GL contained higher crude ash, crude fat, and crude protein content. The most abundant amino acid, fatty acid, mineral, and soluble vitamin observed were valine (round type: 11.90 mg/g and Nokgak type: 17.18 mg/g), linoleic acid (round type: 47.56% and Nokgak type: 75.68%), potassium (round type: 116.50 mg/100 g and Nokgak type: 184.36 mg/100 g), and vitamin B3 (round type: 1.78 mg/100 g and Nokgak type: 1.81 mg/100 g), respectively. In addition, the ${\beta}$-glucan content were 34.15 g/100 g (round type) and 30.07 g/100 g (Nokgak type). The GL 70% ethanol extract at $40^{\circ}C$ showed higher radical scavenging as well as carbohydrate and lipid enzyme inhibition than other conditions. At 1 mg/mL of treatment with the 70% ethanol extract at $40^{\circ}C$ of round type GL, the DPPH, ABTS, hydroxyl radical scavenging, and ${\alpha}$-glucosidase, ${\alpha}$-amylase, and pancreatic lipase inhibition activities obtained were approximately 92.85, 99.74, 58.09, 89.68, 44.68, and 67.56%, respectively.

본 연구는 영지버섯(Ganoderma lucidum)의 영양학적 특성과 생리활성을 조사하였다. 탄수화물 함량은 원형 영지버섯이 높았고 조회분, 조지방, 조단백질 및 무기질 함량은 녹각 영지버섯이 높았다. 주요 아미노산, 지방산, 무기질 및 수용성 비타민은 valine(원형: 11.90 mg/g 및 녹각: 17.18 mg/g), linoleic acid(원형: 47.56% 및 녹각: 75.68%), 칼륨(원형: 116.50 mg/100 g 및 녹각: 184.36 mg/100 g) 및 비타민 $B_3$(원형: 1.78 mg/100 g 및 녹각: 1.81 mg/100 g) 있었다. 한편 ${\beta}$-glucan 함량은 각각 34.15 g/100 g(원형)과 30.07 g/100 g(녹각)로 검출되었다. 추출조건 중 $40^{\circ}C-70%$ 에탄올 추출물에서 가장 우수한 라디칼 소거활성과 탄수화물 및 지방분해 효소 저해활성을 나타내었다. 특히, $40^{\circ}C-70%$ 에탄올로 추출한 원형영지버섯의 1 mg/mL 처리 시 DPPH, ABTS 및 hydroxyl 라디칼 소거활성과 ${\alpha}$-glucosidase, ${\alpha}$-amylase 및 pancreatic lipase 저해활성은 각각 92.85, 99.74, 58.09, 89.68, 44.68, 67.56%로 나타났다.

Keywords

References

  1. Choi SJ, Lee YS, Kim JK, Kim JK, Lim SS (2010) Physiological activities of extract from edible mushrooms. J Korean Soc Food Sci Nutr, 39, 1087-1096
  2. Lee SY, Kim JH, Park JM, Lee IC, Lee JY (2014) Antioxidant activity and inhibition activity against $\alpha$ -amylase and $\alpha$-glucosidase of Smilax China L. Korean J Food Preserv, 21, 254-263
  3. Lee SJ, Park HJ, Song YO, Jang SH, Goo AJ, Ko Y, Cho JH (2014) Antioxidant activity and anti-obesity effect of Coprinus comatus in Zucker rat (fa/fa). Korean J Vet Serv, 37, 51-58
  4. Joo OS (2008) Chemical components and physiological activities of Neungee Mushroom (Sarcodon aspratus). Korean J Food Preserv, 15, 864-871
  5. Lee HJ, Do JR, Jung SK, Kim HK (2014) Physiological properties of Sarcodon aspratus extracts by ethanol concentration. J Korean Soc Food Sci Nutr, 43, 656-660
  6. Lee JK, Song JH, Lee JS (2010) Purification of anti-obesity lipase inhibitor from the fruiting body of Phellinus linteus. Kor J Mycol, 38, 57-61
  7. Lee MR, Hou JG, Begum S, Wang YB, Oh DS, Wi AJ, Yoon BS, Sung CK (2014) Anti-obesity effects of Sparassis crispa on high-fat diet-induced obese mice. J Life Sci, 24, 952-958
  8. Ryu HJ, Um MY, Ahn JY, Jung CH, Huh D, Kim TW, Ha TY (2011) Anti-obesity effect of Hypsizigus marmoreus in high fat-fed mice. J Korean Soc Food Sci Nutr, 40, 1708-1714
  9. Yang BK, Kim DH, Song CH (2002) Production of Lentinus edodes mycelia in submerged culture and it's hypoglycemic effect in diabetic rats. Kor J Mycol, 30, 131-135
  10. Park JS (2013) Effect on the inhibition of pancreatic lipase and lipid metabolism of Zanthoxylum poperitum extracts. Korean J Food Nutr, 26, 615-619
  11. Yoon DE, Park YJ, Kwon OC, Nam JY, Kim HI, Yoo YB, Kong WS, Lee CS (2013) Study on HPLC conditions for chemotaxonomy of Ganoderma species. J Mushroom Sci Prod, 11, 107-110
  12. Cho JH, Noh HJ, Kang DH, Lee JY, Lee MJ, Park HS, Sung GH, Jhune CS (2012) Comparative analysis of amino acid contents of the fruiting bodies in Ganoderma spp.. J Mushroom Sci Prod, 10, 208-215
  13. Cho JH, Lee JY, Lee MJ, Oh HN, Kang DH, Jhune CS (2013) Comparative analysis of useful $\beta$-glucan and polyphenol in the fruiting bodies of Ganoderma spp.. J Mushroom Sci Prod, 11, 164-170
  14. Korea Food Drug Administration. http://fse.foodnara.go.kr/residue/RS/jsp/menu_02_01_01.jsp. (accessed 2010).
  15. Shin EC, Hwang CE, Lee BW, Kim HT, Ko JM, Baek IY, Lee YB, Choi JS, Cho EJ, Seo WT, Cho KM (2012) Chemometric approach to fatty acid profiles in soybean cultivars by principal component analysis (PCA). Prev Nutr Food Sci, 17, 184-191
  16. Folin O Denis W (1912) On phosphotungsticphosphomolybdic compounds as color reagents. J Biol Chem, 12, 239-243
  17. Jia Z, Tang M, Wu J (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem, 64, 555-559
  18. Hwang CE, An MJ, Lee HY, Lee BW, Kim HT, Ko JM, Baek IY, Seo WT, Cho KM (2014) Potential probiotic Lactobacillus plantarum P1201 to produce soy-yogurt with enhanced antioxidant activity. Korean J Food Sci Technol, 46, 556-565
  19. Sin SM, Mok SY, Lee SH, Cho KM, Cho EJ, Kim HY (2011) Protective effect of Bitter Melon (Momordica charantia) against oxidative stress. Cancer Prev Res, 16, 86-92
  20. Park JH, Baek MR, Lee BH, Yon GH, Ryu SY, Kim YS, Park SU, Hong KS (2009) $\alpha$-Glucosidase and $\alpha$-amylase inhibitory activity of compounds from roots extract of Pueraria thunbergiana. Korean J Med Crop Sci, 17, 357-362
  21. Kwon OJ, Lee HY, Kim TH, Kim SG (2014) Antioxidant and pancreatic lipase inhibitory activities of Anemarrhena asphodeloides. Korean J Food Preserv, 21, 421-426
  22. Lee SK, Yoo YJ, Kim CS (1989) Studies on the chemical components in Ganoderma lucidum. Korean J Food Sci Technol, 21, 890-894
  23. Lee HA, Kim BK, Hyun JW (2004) Free amino acids and their derivatives of Ganoderma lucidum. Environ Muta Carcino, 24, 67-72
  24. Lee KJ, Yun IJ, Kim HY, Park YH, Ham HJ, Park YH, Joo JH, Lim SH, Kim KH (2009) Analysis of general components and vitamin and mineral contents of the mushroom Agrocybe chaxingu. Korean J Food Preserv, 16, 549-553
  25. Lee SH, Kim NW, Shin SR (2003) Studies on the nutritional components of mushroom (Sarcodon aspratus). Korean J Food Preserv, 10, 65-69
  26. Kim JH, Lee SC, Ju YC (2007) Effect of far-infrared irradiation on the antioxidant activity of extracts from Phellinus igniarius and Ganoderma lucidum. Korean J Food Sci Technol, 39, 386-389
  27. Jeong GL, Shin HJ, Cha WS (2006) Analysis of amino acids, vitamins and minerals of fruiting body of Fanitopsis pinicola. J Life Sci, 16, 1123-1126
  28. Seo YC, No RH, Kwon HS, Lee HY (2013) Enhancement of the cosmeceutical activity by nano-encapsulation of thiamine di-lauryl sulfate (TDS) with antimicrobial efficacy. J Soc Cosmet Sci Korea, 39, 205-213
  29. Oh SI Lee MS (2005) Antioxidant and antimutagenic effects of Ganoderma lucidum krast extracts. Korean J Food Nutr, 18, 54-62
  30. Hur H (2008) Analysis of biological activities of medicinal mushrooms. Kor J Pharmacogn, 39, 265-269
  31. Cha JY, Jin JS, Cho YS (2011) Biological activity of methanolic extract from Ganoderma lucidum, Momordica charantia, Fagopyrum tataricum, and their mixtures. J Life Sci, 21, 1016-1024
  32. Wang MF, Li JG, Rangarajan MR, Yu Shao J, LaVoie EM, Hung TC, Ho CT (1998) Antioxidative phenolic compounds from sage (Salvia officinalis). J Agric Food Chem, 46, 4869-4873
  33. Cheung LM, Cheung CK, Vincent ECO (2003) Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem, 81, 249-255
  34. Shi M, Zhang Z, Yang Y (2013) Antioxidant and immunoregulatory activity of Ganoderma lucidum polysaccharide (GLP). Carbohydr polym, 95, 200-206
  35. Kim JE, Joo SJ, Seo JH, Lee SP (2009) Antioxidant and $\alpha$-glucosidase inhibitory effect of tartary buckwheat extract obtained by the treatment of different solvents and enzymes. J Korean Soc Food Sci Nutr, 38, 989-995
  36. Park YJ, Nam JY, Yoon DE, Kwon OC, Kim HI, Yoo YB, Kong WS, Lee CS (2013) Comparison of antiinflammatory, antioxidant and anti-allergic effects of Ganoderma species mycelial extracts. J Mushroom Sci Prod, 11, 111-115
  37. Kim SJ, Heo MY, Bae KH, Kang SS, Kim HP (2003) Tyrosinase inhibitory activity of plant extract (III): fatty acid Korean indigenous plants. J Appl Phamacol, 11, 245-248
  38. Oboh G, Ogunsuyi OB, Ogunbadejo MD, Adefegha SA (2016) Influence of gallic acid on $\alpha$-amylase and $\alpha$-glucosidase inhibitory properties of acarbose. J Food Drug Anal, 24, 627-634
  39. Kim MS, Kim BY Park CS, Yoon BD, Ahn SC, Oh WK Ahn JS (2006) Inhibitory effect of Thujae orientalis semen extract on pancreatic lipase activity. J Life Sci, 16, 328-332

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