디지털융복합연구 (Journal of Digital Convergence)

  • 제15권11호
  • /
  • Pages.285-295
  • /
  • 2017
  • /
  • 2713-6434(pISSN)
  • /
  • 2713-6442(eISSN)
한국디지털정책학회 (The Society of Digital Policy and Management)
권호 표지
DOI QR코드

DOI QR Code

증폭시킨 홍삼으로부터 분리한 ginsenoside Rh2, compound K의 융복합적 항암 및 항염효과

Anti-cancer and anti-inflammatory effects of convergence of ginsenoside Rh2, compound K isolated from amplified red ginseng

  • 김영호 (동방문화대학원대학교 자연치유학과) ;
  • 김종두 (동방문화대학원대학교 자연치유학과)
  • Kim, Young-Ho (Dept. of Naturopathic, Dongbang Culture University) ;
  • Kim, Jong-Du (Dept. of Naturopathic, Dongbang Culture University)
  • 투고 : 2017.08.30
  • 심사 : 2017.11.20
  • 발행 : 2017.11.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 증폭시킨 홍삼으로부터 분리한 ginsenoside Rh2(Rh2)와 compound K(CK)의 융복합적 항염증 및 항암효과를 연구하여 홍삼 내 유용한 기능성분에 대한 기초 자료를 제공하고자 한다. 이에 Hep3B에서의 세포독성과 IL-6 유도 STAT3 루시퍼라아제 활성, B16F10과 Hacat 세포의 생존 농도를 측정하였고 Apoptosis와 관련된 분자의 발현양상을 확인하기 위해 FAC (fluorescence activated cell sorting) 분석을 수행하였다. 실험결과 Rh2, CK mixture가 10 ug/ml일 때 Hep3B 세포에서 세포독성이 없고 IL-6 감소율이 102%로 항염증 효과가 있는 것으로 나타났다. 또한 Rh2, CK mixture 50 uM에서 melanoma 세포인 B16F10과 human keratinocyte인 Hacat에서 독성을 보여 사멸하는 것을 관찰하였다. FACS 분석 결과 annexin V가 발현되지 않고 흑색종 세포와 keratinocyte가 탈착되면서 사멸되는 것을 확인하였다. 이러한 현상을 통하여 사멸되는 메카니즘이 anoikis 방식의 세포사멸로 인한 것으로 추정할 수 있으며 그것에 대한 명확한 세포사 신호 체계 규명을 위하여 향후 세포부착 단백질의 변화에 대한 연구가 필요할 것으로 판단된다.

This study aims to provide basic data on useful functional ingredients in red ginseng by studying the anti-inflammatory and anti-cancer effects of convergence of ginsenoside Rh2(Rh2) and compound K(CK) isolated from amplified red ginseng. Therefore we examined cytotoxicity in Hep3B, activity of IL-6 induced STAT3 luciferase and survival concentration of cells in B16F10 and HaCa T. According to the experimental results, when the Rh2 and CK mixture were 10 ug/ml, there was no cytotoxicity in Hep3B cells and the anti-inflammatory effect of IL-6 reduction ratio was 102%. In addition, Rh2 and CK mixture were observed to be toxic in melanoma cell line B16F10 and HaCa T (human keratinocyte) at 50 uM. FACS(fluorescence activated cell sorting) analysis showed that annexin V was not expressed and melanoma cells and keratinocyte were desorbed and killed. It can be assumed that the mechanism of killing through this phenomenon is due to the cell death of anoikis-type, and it is necessary to study the changes of cell adhesion proteins in the future in order to clarify the cell death signal system.

키워드

참고문헌

  1. W. C. Cho, "Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats." Eur. J. Pharmacol. Vol. 550, No. 1, pp.173-179, 2006. https://doi.org/10.1016/j.ejphar.2006.08.056
  2. J. Y. Cho, "Ginsenosides from Panax ginseng differentially regulate lymphocyte proliferation." Planta Med, Vol. 68, No. 6, pp.497-500, 2002. https://doi.org/10.1055/s-2002-32556
  3. A-Young Jang, Yun-Chul Sueng, Joong-gu Ji, "The comparative study on physiological activity of White ginseng, Red ginseng and Black ginseng extract", Journal of Digital Convergence, Vol.14 No.5 pp.459-471, 2016. https://doi.org/10.14400/JDC.2016.14.5.459
  4. Jong Rok Lee, Young Woo Kim , Sung Hui Byun, Sang Chan Kim, Sook Jahr Park, "Anti-inflammatory effects of the fermentation extracts consisting of soybean, red ginseng and Citrus Unshiu Peel", The Korea journal of herbology, Vol.30 No.5, pp.59-65, 2015. https://doi.org/10.6116/KJH.2015.30.5.59.
  5. Byung-Moon Jin1, Min-Kyung Lee, Jun-Seon Lee, Kyung-Yae Hyun, "Anti-inflammatory Effects of Korean red ginseng Extract in formalin-induced Orofacial Pain in Rats", Journal of the Korea Academia-Industrial cooperation Society, Vol.15 No.9, pp.5708-5715, 2014. https://doi.org/10.5762/KAIS.2014.15.9.5708
  6. Seong-Hwa Oh, Soo-Yeon Choi, Nu Rim Lee, Jung No Lee, Dong-Seok Kim, Sang-Hwa Lee, and Sung-Min Park, "Cell migration and Anti-inflammatory Effect of Red Ginseng Extracts Fermented with Laetiporus Sulphureus", Journal of the society of cosmetic scientists of Korea, Vol.40 No.3, pp. 297-305, 2014. https://doi.org/10.15230/SCSK.2014.40.3.297
  7. Ji Su Shin, Jong-Myoung Kim1, Won Gun An, "Anti-inflammatory Effect of Red Ginseng through Regulation of MAPK in Lipopolysaccharidestimulated RAW264.7", Journal of physiology & pathology in Korean Medicine, Vol.26, No.3, pp. 293-300, 2012.
  8. L. P. Christensen, "Ginsenosides: Chemistry, Biosynthesis, Analysis, and Potential Health Effects. Advances in Food and Nutrition Research." Vol.55, pp.1-99, 2008.
  9. Chang-Eui Hong, Su-Yun Lyu, "Anti-inflammatory and Anti-oxidative Effects of Korean Red Ginseng Extract in Human Keratinocytes", Immune network : official journal of the Korean association of immunobiologists, Vol.11 No.1, pp. 42-49, 2011. https://doi.org/10.4110/in.2011.11.1.42
  10. Muhammad Jahangir Hossen, Yong Deog Hong, Kwang-Soo Baek, Sulgi Yoo, Yo Han Hong, Ji Hye Kim, Jeong-Oog Lee, Donghyun Kim, Junseong Park, Jae Youl Cho, "In vitro antioxidative and anti-inflammatory effects of the compound K-rich fraction BIOGF1K, prepared from Panax ginseng", Journal of ginseng research, Vol.41 No.1, pp. 43-51, 2017. https://doi.org/10.1016/j.jgr.2015.12.009
  11. S. Jung, M.S. Lee, Y. Shin, C.T .Kim, I.H. Kim, Y.S. Kim, Y. Kim, "Anti-obesity and anti-inflammatory effects of high hydrostatic pressure extracts of ginseng in high-fat diet induced obese rats", Journal of functional foods : the official journal of the International Society for Nutraceuticals & Functional Foods, Vol.10 , pp. 169-177, 2014
  12. M Oh, Y H Choi, S Choi, H Chung, K Kim, S I Kim, D K Kim, N D Kim, "Anti-proliferating effects of ginsenoside Rh2 on MCF-7 human breast cancer cells", International journal of oncology, Vol.14, No.5, pp. 869-875, 1999.
  13. Ae-Jung Kim, Myung-Rhun Han, Soo-Jeong Lee, "Antioxidative Capacity and Quality Characteristics of Yanggaeng using Fermented Red Ginseng for the Elderly", The Korean journal of food and nutrition, Vol.25, No.1, pp. 83-89, 2012. https://doi.org/10.9799/ksfan.2012.25.1.083
  14. B. M. Kong, "Physico-Chemical Characteristics of White, Fermented and Red Ginseng Extracts." J. Ginseng Res. Vol. 32, No. 3 pp.238-243, 2008. https://doi.org/10.5142/JGR.2008.32.3.238
  15. Hee Kang, Yun-Gu Hwang, Taek-Guen Lee, Cheng-R Jin, Chi Heung Cho, Hee-Yeong Jeong, Dae-Ok Kim, "Use of Gold Nanoparticle Fertilizer Enhances the Ginsenoside Contents and Anti-Inflammatory Effects of Red Ginseng", Journal of microbiology and biotechnology, Vol.26, No.10, pp. 1668-1674, 2016. https://doi.org/10.4014/jmb.1604.04034
  16. Hyun-Joo Jung, Hojin Choi, Hye-Won Lim, Daehyun Shin, Hacksoo Kim, Bin Kwon, Jong Eun Lee, Eun-Hee Park, Chang-Jin Lim, "Enhancement of anti-inflammatory and antinociceptive actions of red ginseng extract by fermentation", Journal of pharmacy and pharmacology, Vol.64, No.5, pp. 756-762, 2012. https://doi.org/10.1111/j.2042-7158.2012.01460.x
  17. Hye-Jin Park, Da-Hye Jung, Haemi Joo, Nam-Sung Kang, Seon-A Jang, Jae Geun Lee, and Eun-Hwa Sohn, "The comparative study of anti-allergic and anti-inflammatory effects by fermented red ginseng and red ginseng" , Korean journal of plant resources, Vol.23, No.5, pp. 415-422, 2010.
  18. Byung-Weon Kim, Yoon-Bok Lee, Jae-Seung Park, Ji-Won Park, Seock-Yeon Hwang, "The Effect of Red Ginseng Extracts on Blood Biochemical Parameters, Bone Density and Bone Inorganic Components in Ovariectomized Rats", The Journal of digital policy & management, Vol.11, No.10, pp. 493-505, 2013.
  19. Byung-Weon Kim, Yoon-Bok Lee, Jae-Seung Park, Ji-Won Park, Seock-Yeon Hwang, "Effect of Red Ginseng Saponin Fraction on the Blood Components of Male Guinea Pigs with Acute Toxicity induced by 2,3,7,8-Tetracholorodibenzo-${\rho}$-dioxin (TCDD)" , The Journal of digital policy & management, Vol.11, No.4, pp. 339-350, 2013.
  20. K. Y. Nam, "The comparative understanding between red ginseng and white ginsengs, processed ginsengs(panax ginseng C. A. Meyer)." J. Ginseng Res, Vol. 29, No. 1, pp.1-18, 2005. https://doi.org/10.5142/JGR.2005.29.1.001
  21. H. Sung, "Beneficial effects of a combination of Korean red ginseng and highly active antiretroviral therapy in human immunodeficiency virus type 1-infected patients." Clin. Vaccine Immunol. Vol. 16, No. 8, pp.1127-1131, 2009. https://doi.org/10.1128/CVI.00013-09
  22. E. A. Bae, "antiallergic and antipsoriatic effects of korean red ginseng." J. Ginseng Res, Vol. 29, No. 2, pp.80-85, 2005. https://doi.org/10.5142/JGR.2005.29.2.080
  23. D. J. Jang, "Red ginseng for treating erectile dysfunction: a systematic review." Br. J. Clin. Pharmacol, Vol. 66, No. 4, pp.444-450, 2008. https://doi.org/10.1111/j.1365-2125.2008.03236.x
  24. Jongsun Park, Jae Youl Cho, "Anti-inflammatory effects of ginsenosides from Panax ginseng and their structural analogs", African journal of biotechnology, Vol.8, No.16, pp. 3682-3690, 2009
  25. Frisch SM, Screaton RA, "Anoikis mechanisms", Current Opinion in Cell Biology, Vol.13, No.5, pp.555-562,2001, http://dx.doi.org/10.1016/S0955-0674(00)00251-9