Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Enzyme Activity and Anti-Adipogenic Effects of (-)-Epigallocatechin-3-Gallate in 3T3-L1 Cells

(-)-Epigallocatechin-3-Gallate의 3T3-L1 세포에서 항산화 효소 활성 및 지방세포 분화 억제 효과

  • Kim, Younghwa (School of Food Biotechnology and Nutrition, Kyungsung University)
  • 김영화 (경성대학교 식품응용공학부)
  • Received : 2017.08.02
  • Accepted : 2017.08.08
  • Published : 2017.11.30
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Abstract

Obesity contributes to the development of diseases, such as type II diabetes, hypertension, coronary heart disease, and cancer. In addition, oxidative stress caused by reactive oxygen species (ROS) is recognized widely as a contributing factor in the development of chronic diseases. This study was examined the antioxidant and anti-adipogenic activities of epigallocatechin-3-gallate (EGCG) in 3T3-L1 preadipocytes. 3T3-L1 cells were differentiated with or without EGCG for 6 days. The production of glutathione (GSH) and the activities of the antioxidant enzymes, such as glutathione reductase (GR), glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD) were measured. EGCG inhibited significantly the lipid accumulation and the expression of adipogenic specific proteins including CCAAT/enhancer binding protein ${\alpha}$ and adipocyte fatty acid binding protein. The production of intracellular ROS was decreased significantly by EGCG in 3T3-L1 cells. EGCG increased the GSH production and the activities of GPx, GR, CAT, and SOD. Moreover, EGCG increased the protein expression of glutamate-cysteine ligase and heme oxygenase-1 in 3T3-L1 cells. These results suggest that EGCG increased the activity and expression of antioxidant enzymes and suppressed the lipid accumulation in 3T3-L1 cells. Therefore, the use of phytochemicals that can maintain the GSH redox balance in adipose tissue could be promising for reducing obesity.

Epigallocatechin-3-gallate(EGCG)는 녹차에 함유된 flavonoid 계열 화합물로, 녹차의 대표적인 유효성분으로 알려져 있다. 본 연구는 3T3-L1 세포에서 EGCG의 항비만 작용 및 항산화 효소 활성을 평가하였다. EGCG는 지방세포 분화를 유의적으로 억제하였으며, 지방세포 분화와 관련된 단백질인 $C/EBP{\alpha}$와 aP2의 발현을 감소시켰다. 동시에 EGCG는 GSH의 활성을 유도하였으며, 항산화 효소인 SOD, CAT, GPx 및 GR의 활성을 증가시켰다. EGCG는 지방세포 분화 동안 활성산소종의 형성을 감소시켰다. 체내 phase II 효소인 HO-1과 GCLC는 EGCG에 의해 발현이 유도되었다. 따라서 EGCG는 3T3-L1 세포에서 항산화 효소의 활성화를 유도하며 동시에 지방세포로의 분화를 억제하는 것으로 나타났다. 또한, EGCG는 활성산소종의 생성을 억제하였으며, 이는 EGCG의 항산화 작용에 의한 지방세포 분화 억제 작용이 관련이 있는 것으로 판단된다. EGCG에 의한 항비만 효과와 항산화 작용을 연구한 본 연구는 녹차를 활용한 체지방 감소 효능을 지닌 식품 개발에 기초 자료로 활용될 수 있을 것으로 생각된다.

Keywords

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