The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Natural Herb Mixture on Anti-atopic Dermatitis and Skin Regeneration

천연 소재 복합물이 항아토피 피부염 및 피부재생에 미치는 영향

  • Kim, Won-Sik (Sinwolmaek Korean Medicine Clinic) ;
  • Sim, Boo-Yong (Traditional and Biomedical Research Center(TBRC-RIC), Daejeon University) ;
  • Kim, Dong-Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
  • 김원식 (신월맥 한의원) ;
  • 심부용 (대전대학교 난치성 면역질환의 동서생명의학연구 지역혁신센터) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Received : 2017.10.03
  • Accepted : 2017.11.15
  • Published : 2017.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study aims to evaluate the effects of natural herb mixutre (NHM) on atopic dermatitis and skin regeneration using in vivo test. Methods : NHM was prepared with DW. 25% of NHM was applied to skin lesion, where atopic dermatitis was induced by DNCB in NC/Nga mice. The levels of cytokines (IL-4, IL-5, IL-6, IL-13, TNF-a, and $IFN-{\gamma}$), and IgE in serum were measured by Luminex. Immune cells (WBC, eosinophil, lymphocyte, and monocyte) in blood were counted by coulter counter. The gross investigation of atopic dermatitis index score test were performed during the NHM treatment period. Also, the histopathological change of dorsal skin was observed by H&E and M&T staining. Results : NHM showed the levels of IL-4, IL-5, IL-6, IL-13, IgE, WBC, eosinophil, lymphocyte, and monocyte in serum or blood were significantly decreased. On the contrary, the productions of FGF, and VEGF were increased in the serum. Also, atopic dermatitis index score in NHM-treated mice were observed in the similar levels to those of normal group. Histological examination demonstrated that NHM suppressed immune cell infiltration and thickening of epidermis, meanwhile the extraction induced collagen production in the dorsal skin. Conclusion : This study demonstrated that NHM is appeared to be effective on atopic dermatitis and skin regeneration efficacy based on the observations with hematologic, gross, and histologic examinations. Therefore, we suggest that NHM could be effectively used as an external therapeutics against atopic dermatitis and a consequence skin damage.

Keywords

References

  1. Morales-Suarez-Varela M, Gallardo-Juan A, Garcia- Marcos L, Gimeno-Clemente N, Lopez-Silvarrey- Varela A, Miner-Canflanca I, Batlles-Garrido J, Blanco-Quiros A, Busquets-Monge RM, Dominguez- Aurrecoechea B, Arnedo-Pena A. The impact of atmospheric pollutants on the prevalence of atopic eczema in 6-7-year-old schoolchildren in Spain; ISAAC Phase III. Iranian Journal of Allergy, Asthma and Immunology. 2013 ; 12(3) : 220.
  2. Korean statistical information sevice. Statistic. Available from : URL : http://kosis.kr/statisticsList/statisticsList_01List.jsp?vwcd=MT_ZTITLE&parentI d=D#SubCont
  3. Wu-qian. yi-zong-jin-jian. China : Renminweisheng publisher. 1963. p 1890.
  4. Lassus. Clinical comparison of alclometasone dipropionate cream 0.05% with hydrocortisone butyrate cream 0.1% in the treatment of atopic dermatitis in children. J Int Med Res. 1983 ; 11(5) : 315-9. https://doi.org/10.1177/030006058301100512
  5. Callen J, Chamlin S, Eichenfield LF, Ellis C, Girardi M, Goldfarb M, Hanifin J, Lee P, Margolis D, Paller AS, Piacquadio D, A systematic review of the safety of topical therapies for atopic dermatitis. British Journal of Dermatology. 2007 ; 156(2) : 203-21. https://doi.org/10.1111/j.1365-2133.2006.07538.x
  6. Pariser D. Topical corticosteroids and topical calcineurin inhibitors in the treatment of atopic dermatitis: focus on percutaneous absorption. Am J Ther. 2009 ; 16(3) : 264-73. https://doi.org/10.1097/MJT.0b013e31818a975c
  7. Breuer K, Werfel T, Kapp A. Safety and efficacy of topical calcineurin inhibitors in the treatment of childhood atopic dermatitis. American journal of clinical dermatology. 2005 ; 6(2) : 65-77. https://doi.org/10.2165/00128071-200506020-00001
  8. Frankel HC, Qureshi AA. Comparative effectiveness of topical calcineurin inhibitors in adult patients with atopic dermatitis. American journal of clinical dermatology. 2012 ; 13(2) : 113-23. https://doi.org/10.2165/11597780-000000000-00000
  9. Tennis P, Gelfand JM, Rothman KJ. Evaluation of cancer risk related to atopic dermatitis and use of topical calcineurin inhibitors. British Journal of Dermatology. 2011 ; 165(3) : 465-73. https://doi.org/10.1111/j.1365-2133.2011.10363.x
  10. Lee DJ, Jeon IH, Kim HS, Cho IY, Jang SI. Antioxidative and anti-inflammatory effect of ethanol extract from duchesnea chrysantha. Journal of Physiology & Pathology in Korean Medicine. 2012 ; 26(1) : 59-66.
  11. Lee DJ, Cho IY, Jang SI. Anti-allergy Effect of Ethanol Extract from Duchesnea chrysantha. Journal of Physiology & Pathology in Korean Medicine. 2012; 26(6) : 902-7.
  12. Kang SH, Kim GJ. Effects of Lithospermum erythrorhizon on the cytokine gene expression in human keratinocytes. The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology. 2013 ; 26(1) : 50-62. https://doi.org/10.6114/jkood.2013.26.1.050
  13. Choi SB, Bae GS, Jo IJ, Park KC, Seo SH, Kim DG, Shin JY, Gwak TS, Lee JH, Lee GS, Park SJ. The anti-inflammatory effect of Lithospermum Erythrorhizon on lipopolysaccharide-induced inflammatory response in RAW 264.7 cells. The Korea Journal of Herbology. 2013 ; 28(2) : 67-73. https://doi.org/10.6116/kjh.2013.28.2.67
  14. Kim SH, Jung HS, Lee JY, Kim DG, Cho BG. Effect of Lithospermum erythrorhizon on the atopic dermatitis. The Journal of Pediatrics of Korean Medicine. 2004 ; 18(1) : 63-75.
  15. Lee JY, Park SS. The Effect of Allergic Inflamation by Sophora Flavescens Aiton Extract Ion Through Inhibition of the $NF{\kappa}B$, JNK and p38 Pathway. Journal of Sasang Constitutional Medicine. 2009 ; 21(1) : 139-49.
  16. Jeong SI, Choi BM, Yun YG, Lee JW, Jang SI. A noble therapeutic approach of atopic dermatitis by development of Th2 chemokine inhibitors from natural products: inhibitory effect of Sophora flavescens extract in atopic dermatitis model mice, NC/Nga. Herbal Formula Science. 2009 ; 17(1) : 141-51.
  17. Yu ML, Koo YS, Kim DH. Effects of Sophorae Radix (SFR) on Allergic Inflammation related Atopy Dermatitis. The Journal of Daejeon Oriental Medicine. 2006 ; 15(2) : 135-48.
  18. Katiyar SK, Ahmad N, Mukhtar H. Green tea and skin. Archives of Dermatology. 2000 ; 136(8) : 989-94.
  19. Katiyar SK. Skin photoprotection by green tea: antioxidant and immunomodulatory effects. Current Drug Targets-Immune, Endocrine & Metabolic Disorders. 2003 ; 3(3) : 234-42. https://doi.org/10.2174/1568008033340171
  20. Seo YA. Analgesic and anti-inflammatory activity of Resina Pini. Natural Product Sciences. 2007 ; 13(4) : 347-54.
  21. Jung SA, Choi YY, Yang WM. Anti-atopic dermatitis effects of Rafinesque via regulation of immune response and nerve growth factor. Journal of Korean Medicine. 2016 ; 37(1) : 10-20. https://doi.org/10.13048/jkm.16002
  22. Park JM, Chae JW. Effects of Aurantii Immaturus Fructus (AI) on Atopic Dermatitis (AD) Induced by DNCB in Mice. The Journal of Pediatrics of Korean Medicine. 2015 ; 29(1) : 27-43. https://doi.org/10.7778/jpkm.2015.29.1.027
  23. Kim HK, Lee EJ, Kweon YT, Hwang KH, Joo HH, Song BK. The Study on the antiallergic Action of Poncirus trifoliata. The Journal of Korean Oriental Internal Medicine. 2000 ; 21(1) : 156-61.
  24. Koblenzer CS. Itching and the Atopic Skin. J. Allerrgy Clin. Immun. 1999 ; 104(3) : S109-13. https://doi.org/10.1016/S0091-6749(99)70052-7
  25. Mihara K, Kuratani K, Matusui T, Yokota K. Vital Role of the Itch-scratch Response in Development of Spontaneous Dermatitis in NC/Nga Mice. J. Dermatol, 2004 ; 151(2) : 335-45. https://doi.org/10.1111/j.1365-2133.2004.06036.x
  26. Kim GW, Kim DH. Effects of Ato-tang on DNCBinduced atopic dermatitis in Mice. The Korea Journal of Herbology. 2016 ; 31(4) : 79-85. https://doi.org/10.6116/kjh.2016.31.4.79.
  27. Kim HJ, Shin JU, Lee KH. Atopic dermatitis and skin barrier dysfunction. Allergy asthma respir dis. 2013 ; 1(1) : 20-8. https://doi.org/10.4168/aard.2013.1.1.20
  28. Kubo A, Nagao K, Amagai M. Epidermal barrier dysfunction and cutaneous sensitization in atopic diseases. The Journal of clinical investigation. 2012 ; 122(2) : 440-7. https://doi.org/10.1172/JCI57416
  29. Hatano Y, Terashi H, Arakawa S, Katagiri K. Interleukin-4 suppresses the enhancement of ceramide synthesis and cutaneous permeability barrier functions induced by tumor necrosis factor-${\alpha}$ and interferon-${\gamma}$ in human epidermis. Journal of investigative dermatology. 2005 ; 124(4) : 786-92. https://doi.org/10.1111/j.0022-202X.2005.23651.x
  30. Kim BE, Leung DY, Boguniewicz M, Howell MD. Loricrin and involucrin expression is downregulated by Th2 cytokines through STAT-6. Clinical Immunology. 2008 ; 126(3) : 332-7. https://doi.org/10.1016/j.clim.2007.11.006
  31. Ki HP, Jang SI, Yun YG. Ameliorative Effects of Hwangnyeonhaedok-tang on Atopic Dermatitis. Herbal formula science. 2013 ; 21(1) : 80-90. https://doi.org/10.14374/HFS.2013.21.1.080
  32. Abramovits W. Atopic dermatitis. J am acad dermatol. 2005 ; 53(1) : 86-93. https://doi.org/10.1016/j.jaad.2005.04.034
  33. Sim BY, Kim SH, Kim DH. Anti-inflammatory effect of Baecksunpijibujabokhap-bang in Atopic dermatitis model mice. The Korea Journal of Herbology. 2014 ; 29(3) : 51-8. https://doi.org/10.6116/kjh.2014.29.3.51
  34. Lee BW, Gim SB, Song HH, Ji JG, Bak JW, Kim DH. Study on the immune modulatory activity of seokjahaeki-tang using atopic dermatitis animal models. Korean J. Oriental physiology & pathology. 2012 ; 26(4) : 446-54.
  35. Li J, Chen J, Kirsner R. Pathophysiology of acute wound healing. Clinics in dermatology. 2007 ; 25(1) : 9-18. https://doi.org/10.1016/j.clindermatol.2006.09.007
  36. Kirsner RS, Eaglstein WH. The wound healing process. Dermatologic clinics. 1993 ; 11(4) : 629-40. https://doi.org/10.1016/S0733-8635(18)30216-X
  37. Singer AJ, Clark RA. Cutaneous wound healing. New England journal of medicine. 1999 ; 341(10) : 738-46. https://doi.org/10.1056/NEJM199909023411006