Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase

  • Lee, Jin-Moo (Department of Applied Chemistry, Kookmin University) ;
  • Cho, Jin-Beom (Department of Applied Chemistry, Kookmin University) ;
  • Ahn, Hee-Chul (Department of Pharmacy, Dongguk University-Seoul) ;
  • Jung, Woong (Department of Emergency Medicine, Kyung Hee University Hospital at Gangdong) ;
  • Jeong, Yong-Joo (Department of Applied Chemistry, Kookmin University)
  • Received : 2017.07.31
  • Accepted : 2017.09.05
  • Published : 2017.11.28
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Abstract

We have discovered a novel chemical compound, (E)-3-(furan-2-yl)-N-(4-sulfamoylphenyl) acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we obtained $IC_{50}$ values of $2.09{\pm}0.30{\mu}M$ (ATP hydrolysis) and $13.2{\pm}0.9{\mu}M$ (DNA unwinding), respectively. Moreover, we found that the compound did not have any significant cytotoxicity when $40{\mu}M$ of it was used. Our results showed that the compound might be useful to be developed as an inhibitor against SARS coronavirus.

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References

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