Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of dentin biomodifiers on the immediate and long-term bond strengths of a simplified etch and rinse adhesive to dentin

  • Singh, Payal (Department of Conservative Dentistry and Endodontics, Kothiwal Dental College and Research Center) ;
  • Nagpal, Rajni (Department of Conservative Dentistry and Endodontics, Kothiwal Dental College and Research Center) ;
  • Singh, Udai Pratap (Department of Conservative Dentistry and Endodontics, Kothiwal Dental College and Research Center)
  • Received : 2016.12.03
  • Accepted : 2017.03.15
  • Published : 2017.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: This in vitro study evaluated the effect of dentin biomodifiers on the immediate and long-term bond strengths of a simplified etch and rinse adhesive to dentin. Materials and Methods: Flat coronal dentin surfaces were prepared in 120 extracted human molars. Teeth were randomly divided into 5 groups (n = 24) according to 5 different surface pre-treatments: No pre-treatment (control); 1M carbodiimide (EDC); 0.1% epigallocatechin-3-gallate (EGCG); 2% minocycline (MI); 10% sodium ascorbate (SA). After surface pre-treatment, adhesive (Adper Single Bond 2 [SB], 3M ESPE) was applied. Composite was applied into transparent plastic tubes (2.5 mm in diameter), which was placed over the bonded dentin surface. From each group, 10 samples were subjected to shear bond strength (SBS) evaluation at 24 hours (immediate) and remaining 10 samples were tested after 6 months (delayed). Additionally, 4 samples per group were subjected to scanning electron microscopic analysis for observation of resin-dentin interface. The data were statistically analysed with Shaperio-Wilk W test, 2-way analysis of variance (ANOVA), and post hoc Tukey's test. Results: At 24 hours, SBS of all surface pre-treatment groups were comparable with the control group, with significant differences found between EDC and SA groups only (p = 0.009). After 6 months storage, EDC, EGCG, and MI pre-treatments preserved the resindentin bond strength with no significant fall. Conclusions: Dentin pre-treatment with all the dentin biomodifiers except SA resulted in significant preservation of resin-dentin bond over 6 months storage period, without negatively affecting the immediate bond strength of the etch and rinse adhesive tested.

Keywords

References

  1. Hashimoto M, Ohno H, Kaga M, Endo K, Sano H, Oguchi H. In vivo degradation of resin-dentin bonds in humans over 1 to 3 years. J Dent Res 2000;79:1385-1391. https://doi.org/10.1177/00220345000790060601
  2. Yang B, Adelung R, Ludwig K, Bossmann K, Pashley DH, Kern M. Effect of structural change of collagen fibrils on the durability of dentin bonding. Biomaterials 2005;26:5021-5031. https://doi.org/10.1016/j.biomaterials.2005.01.024
  3. Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, Ito S. Collagen degradation by host-derived enzymes during aging. J Dent Res 2004;83:216-221. https://doi.org/10.1177/154405910408300306
  4. Pashley DH, Tay FR, Breschi L, Tjaderhane L, Carvalho RM, Carrilho M, Tezvergil-Mutluay A. State of the art etch-and-rinse adhesives. Dent Mater 2011;27:1-16. https://doi.org/10.1016/j.dental.2010.10.016
  5. Bedran-Russo AK, Pauli GF, Chen SN, McAlpine J, Castellan CS, Phansalkar RS, Aguiar TR, Vidal CM, Napotilano JG, Nam JW, Leme AA. Dentin biomodification: strategies, renewable resources and clinical applications. Dent Mater 2014;30:62-76. https://doi.org/10.1016/j.dental.2013.10.012
  6. Monteiro TM, Basting RT, Turssi CP, Franca FM, Amaral FL. Influence of natural and synthetic metalloproteinase inhibitors on bonding durability of an etch-and-rinse adhesive to dentin. Int J Adhes Adhes 2013;47:83-88. https://doi.org/10.1016/j.ijadhadh.2013.09.020
  7. Castellan CS, Bedran-Russo AK, Antunes A, Pereira PN. Effect of dentin biomodification using naturally derived collagen cross-linkers: one-year bond strength study. Int J Dent 2013;2013:918010.
  8. Erhardt MC, Osorio R, Viseras C, Toledano M. Adjunctive use of an anti-oxidant agent to improve resistance of hybrid layers to degradation. J Dent 2011;39:80-87. https://doi.org/10.1016/j.jdent.2010.10.013
  9. Khamverdi Z, Rezaei-Soufi L, Rostamzadeh T. The effect of epigallocatechin gallate on the dentin bond durability of two self-etch adhesives. J Dent (Shiraz) 2015;16:68-74.
  10. Hiraishi N, Sono R, Sofiqul I, Yiu C, Nakamura H, Otsuki M, Takatsuka T, Tagami J. In vitro evaluation of plant-derived agents to preserve dentin collagen. Dent Mater 2013;29:1048-1054. https://doi.org/10.1016/j.dental.2013.07.015
  11. Sorsa T, Tjaderhane L, Konttinen YT, Lauhio A, Salo T, Lee HM, Golub LM, Brown DL, Mantyla P. Matrix metalloproteinases: contribution to pathogenesis, diagnosis and treatment of periodontal inflammation. Ann Med 2006;38:306-321. https://doi.org/10.1080/07853890600800103
  12. Hass V, de Paula AM, Parreiras S, Gutierrez MF, Luque-Martinez I, de Paris Matos T, Bandeca MC, Loguercio AD, Yao X, Wang Y, Reis A. Degradation of dentin-bonded interfaces treated with collagen cross-linking agents in a cariogenic oral environment: an in situ study. J Dent 2016;49:60-67. https://doi.org/10.1016/j.jdent.2016.02.009
  13. Roomi MW, Ivanov V, Kalinovsky T, Niedzwiecki A, Rath M. Inhibition of matrix metalloproteinase-2 secretion and invasion by human ovarian cancer cell line SK-OV-3 with lysine, proline, arginine, ascorbic acid and green tea extract. J Obstet Gynaecol Res 2006;32:148-154. https://doi.org/10.1111/j.1447-0756.2006.00389.x
  14. Gotti VB, Feitosa VP, Sauro S, Correr-Sobrinho L, Leal FB, Stansbury JW, Correr AB. Effect of antioxidants on the dentin interface bond stability of adhesives exposed to hydrolytic degradation. J Adhes Dent 2015;17:35-44.
  15. Khamverdi Z, Rezaei-Soufi L, Kasraei S, Ronasi N, Rostami S. Effect of Epigallocatechin Gallate on shear bond strength of composite resin to bleached enamel: an in vitro study. Restor Dent Endod 2013;38:241-247. https://doi.org/10.5395/rde.2013.38.4.241
  16. Meharry MR, Moazzami SM, Li Y. Comparison of enamel and dentin shear bond strengths of current dental bonding adhesives from three bond generations. Oper Dent 2013;38:E237-E245. https://doi.org/10.2341/12-521-L
  17. Scheffel DL, Delgado CC, Soares DG, Basso FG, de Souza Costa CA, Pashley DH, Hebling J. Increased durability of resin-dentin bonds following cross-linking treatment. Oper Dent 2015;40:533-539. https://doi.org/10.2341/13-211-L
  18. Mobarak EH. Effect of chlorhexidine pretreatment on bond strength durability of caries-affected dentin over 2-year aging in artificial saliva and under simulated intrapulpal pressure. Oper Dent 2011;36:649-660. https://doi.org/10.2341/11-018-L
  19. Montagner AF, Sarkis-Onofre R, Pereira-Cenci T, Cenci MS. MMP inhibitors on dentin stability: a systematic review and meta-analysis. J Dent Res 2014;93:733-743. https://doi.org/10.1177/0022034514538046
  20. Tezvergil-Mutluay A, Agee KA, Hoshika T, Carrilho M, Breschi L, Tjaderhane L, Nishitani Y, Carvalho RM, Looney S, Tay FR, Pashley DH. The requirement of zinc and calcium ions for functional MMP activity in demineralized dentin matrices. Dent Mater 2010;26:1059-1067. https://doi.org/10.1016/j.dental.2010.07.006
  21. Scheffel DL, Hebling J, Agee KA, De Souza Costa CA, Pashley DH. Collagen degradation and MMP activity in dentin biomodified by cross-linkers. Dent Mater 2014;30 Supplement 1:e158. https://doi.org/10.1016/j.dental.2014.08.322
  22. Scheffel DL, Hebling J, Scheffel RH, Agee KA, Cadenaro M, Turco G, Breschi L, Mazzoni A, Costa CA, Pashley DH. Stabilization of dentin matrix after cross-linking treatments, in vitro. Dent Mater 2014;30:227-233. https://doi.org/10.1016/j.dental.2013.11.007
  23. Seseogullari-Dirihan R, Tjaderhane L, Breschi L, Vallittu P, Pashley DH, Tezvergil-Mutluay A. Effect of pretreatment pH on dentin protease inactivation by carbodiimide. Dent Mater 2012;28 Supplement 1:e64.
  24. Angeloni V, Mazzoni A, Frassetto A, Cadenaro M, Falconi M, Manzoli L, Pashley DH, Breschi L. EDC stabilize the adhesive interface over time. Dent Mater 2013;29 Supplement 1:e65-e66.
  25. Cadenaro M, Fontanive L, Navarra CO, Gobbi P, Mazzoni A, Di Lenarda R, Tay FR, Pashley DH, Breschi L. Effect of carboidiimide on thermal denaturation temperature of dentin collagen. Dent Mater 2016;32:492-498. https://doi.org/10.1016/j.dental.2015.12.006
  26. Tezvergil-Mutluay A, Mutluay MM, Agee KA, Seseogullari-Dirihan R, Hoshika T, Cadenaro M, Breschi L, Vallittu P, Tay FR, Pashley DH. Carbodiimide cross-linking inactivates soluble and matrix-bound MMPs, in vitro. J Dent Res 2012;91:192-196. https://doi.org/10.1177/0022034511427705
  27. Scheffel DL, Hebling J, Scheffel RH, Agee K, Turco G, de Souza Costa CA, Pashley D. Inactivation of matrix-bound matrix metalloproteinases by cross-linking agents in acid-etched dentin. Oper Dent 2014;39:152-158. https://doi.org/10.2341/12-425-L
  28. Perumal S, Antipova O, Orgel JP. Collagen fibril architecture, domain organization, and triple-helical conformation govern its proteolysis. Proc Natl Acad Sci U S A 2008;105:2824-2829. https://doi.org/10.1073/pnas.0710588105
  29. da Fonseca BM, Pleffken PR, Balducci I, Pucci CR, Tay FR, de Araujo MA. New trends in dentin bonding: treatment with Chlorhexidine, Hyaluronic acid, vitamin C and green tea. Braz Dent Sci 2013;16:56-62.
  30. Santiago SL, Osorio R, Neri JR, Carvalho RM, Toledano M. Effect of the flavonoid epigallocatechin-3-gallate on resin-dentin bond strength. J Adhes Dent 2013;15:535-540.
  31. Zheng P, Zaruba M, Attin T, Wiegand A. Effect of different matrix metalloproteinase inhibitors on microtensile bond strength of an etch-and-rinse and a self-etching adhesive to dentin. Oper Dent 2015;40:80-86. https://doi.org/10.2341/13-162-L
  32. Carvalho C, Fernandes FP, Freitas VP, Franca FM, Basting RT, Turssi CP, Amaral FL. Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin. J Appl Oral Sci 2016;24:211-217. https://doi.org/10.1590/1678-775720150518
  33. Stanislawczuk R, Costa JA, Polli LG, Reis A, Loguercio AD. Effect of tetracycline on the bond performance of etch-and-rinse adhesives to dentin. Braz Oral Res 2011;25:459-465. https://doi.org/10.1590/S1806-83242011000500014
  34. Li H, Li T, Li X, Zhang Z, Li P, Li Z. Morphological effects of MMPs inhibitors on the dentin bonding. Int J Clin Exp Med 2015;8:10793-10803.
  35. Pinnel SR, Murad S, Darr D. Induction of collagen synthesis by ascorbic acid. A possible mechanism. Arch Dermatol 1987;123:1684-1686. https://doi.org/10.1001/archderm.1987.01660360122023

Cited by

  1. Polyphenols in Dental Applications vol.7, pp.3, 2017, https://doi.org/10.3390/bioengineering7030072
  2. Effect of Curcumin Suspension and Vitamin C on Dentin Shear Bond Strength and Durability. A Pilot Study vol.15, pp.1, 2017, https://doi.org/10.2174/1874210602115010540