Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Genome scan linkage analysis identifies a major quantitative trait loci for fatty acid composition in longissimus dorsi muscle in an F2 intercross between Landrace and Korean native pigs

  • Park, Hee-Bok (Subtropical Livestock Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Han, Sang-Hyun (Educational Science Research Institute, Jeju National University) ;
  • Yoo, Chae-Kyoung (Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Lee, Jae-Bong (Korea Zoonosis Research Institute, Chonbuk National University) ;
  • Kim, Ji-Hyang (Subtropical Livestock Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Baek, Kwang-Soo (Subtropical Livestock Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Son, Jun-Kyu (Subtropical Livestock Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Shin, Sang-Min (Subtropical Livestock Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Lim, Hyun-Tae (Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Cho, In-Cheol (Subtropical Livestock Research Institute, National Institute of Animal Science, Rural Development Administration)
  • Received : 2016.07.22
  • Accepted : 2016.12.29
  • Published : 2017.08.01
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Abstract

Objective: This study was conducted to locate quantitative trait loci (QTL) influencing fatty acid (FA) composition in a large $F_2$ intercross between Landrace and Korean native pigs. Methods: Eighteen FA composition traits were measured in more than 960 $F_2$ progeny. All experimental animals were genotyped with 165 microsatellite markers located throughout the pig autosomes. Results: We detected 112 QTLs for the FA composition; Forty seven QTLs reached the genome-wide significant threshold. In particular, we identified a cluster of highly significant QTLs for FA composition on SSC12. QTL for polyunsaturated fatty acid on pig chromosome 12 (F-value = 97.2 under additive and dominance model, nominal p-value $3.6{\times}10^{-39}$) accounted for 16.9% of phenotypic variance. In addition, four more QTLs for C18:1, C18:2, C20:4, and monounsaturated fatty acids on the similar position explained more than 10% of phenotypic variance. Conclusion: Our findings of a major QTL for FA composition presented here could provide helpful information to locate causative variants to improve meat quality traits in pigs.

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References

  1. Lawrence TLJ, Fowler VR. Growth of farm animals. 2nd ed. Wallingford, UK: CABI Publishing; 2002. p. 1-347.
  2. Wood JD, Enser M, Fisher AV, et al. Fat deposition, fatty acid composition and meat quality: A review. Meat Sci 2008;78:343-58. https://doi.org/10.1016/j.meatsci.2007.07.019
  3. Kurushima H, Hayashi K, Toyota Y, Kambe M, Kajiyama G. Comparison of hypocholesterolemic effects induced by dietary linoleic acid and oleic acid in hamsters. Atherosclerosis 1995;114:213-21. https://doi.org/10.1016/0021-9150(94)05486-3
  4. Saha SS, Chakraborty A, Ghosh S, Ghosh M. Comparative study of hypocholesterolemic and hypolipidemic effects of conjugated linolenic acid isomers against induced biochemical perturbations and aberration in erythrocyte membrane fluidity. Eur J Nutr 2012;51:483-95. https://doi.org/10.1007/s00394-011-0233-0
  5. Park JC, Kim YH, Jung HJ, et al. Comparison of meat quality and physicochemical characteristics of pork between Korean native black pigs, (KNBP) and Landrace by market weight. J Anim Sci Technol 2005;47:91-8. https://doi.org/10.5187/JAST.2005.47.1.091
  6. Cho IC, Yoo CK, Lee JB, et al. Genome-wide QTL analysis of meat quality-related traits in a large $F_2$ intercross between Landrace and Korean native pigs. Genet Sel Evol 2015;47:7. https://doi.org/10.1186/s12711-014-0080-6
  7. Cho IC, Park HB, Yoo CK, et al. QTL analysis of white blood cell, platelet and red blood cell-related traits in an $F_2$ intercross between Landrace and Korean native pigs. Anim Genet 2011;42:621-6. https://doi.org/10.1111/j.1365-2052.2011.02204.x
  8. Maharani D, Park HB, Lee JB, et al. Association of the gene encoding stearoyl-CoA desaturase (SCD) with fatty acid composition in an intercross population between Landrace and Korean native pigs. Mol Biol Rep 2013;40:73-80. https://doi.org/10.1007/s11033-012-2014-0
  9. Churchill GA, Doerge RW. Empirical threshold values for quantitative trait mapping. Genetics 1994;138:963-71.
  10. Dupuis J, Siegmund D. Statistical methods for mapping quantitative trait loci from a dense set of markers. Genetics 1999;151:373-86.
  11. Zimmerman AW, Veerkamp JH. New insights into the structure and function of fatty acid-binding proteins. Cell Mol Life Sci 2002;59: 1096-116. https://doi.org/10.1007/s00018-002-8490-y
  12. Naganuma T, Sato Y, Sassa T, Ohno Y, Kihara A. Biochemical characterization of the very long-chain fatty acid elongase ELOVL7. FEBS Lett 2011;585:3337-41. https://doi.org/10.1016/j.febslet.2011.09.024
  13. Corominas J, Ramayo-Caldas Y, Puig-Oliveras A, et al. Polymorphism in the ELOVL6 gene is associated with a major QTL effect on fatty acid composition in pigs. PLoS One 2013;8:e53687. https://doi.org/10.1371/journal.pone.0053687
  14. Gregersen N, Andresen BS, Corydon MJ, et al. Mutation analysis in mitochondrial fatty acid oxidation defects: exemplified by acyl-CoA dehydrogenase deficiencies, with special focus on genotype-phenotype relationship. Hum Mutat 2001;18:169-89. https://doi.org/10.1002/humu.1174
  15. Ramayo-Caldas Y, Mercade A, Castello A, et al. Genome-wide association study for intramuscular fatty acid composition in an Iberian ${\times}$ Landrace cross. J Anim Sci 2012;90:2883-93. https://doi.org/10.2527/jas.2011-4900
  16. Sanchez MP, Iannuccelli N, Basso B, et al. Identification of QTL with effects on intramuscular fat content and fatty acid composition in a Duroc ${\times}$ Large White cross. BMC Genet 2007;8:55.
  17. Guo T, Ren J, Yang K, et al. Quantitative trait loci for fatty acid composition in longissimus dorsi and abdominal fat: results from a White Duroc ${\times}$ Erhualian intercross $F_2$ population. Anim Genet 2009;40: 185-91. https://doi.org/10.1111/j.1365-2052.2008.01819.x
  18. Estelle J, Fernandez AI, Perez-Enciso M, et al. A non-synonymous mutation in a conserved site of the MTTP gene is strongly associated with protein activity and fatty acid profile in pigs. Anim Genet 2009;40:813-20. https://doi.org/10.1111/j.1365-2052.2009.01922.x
  19. Zhang W, Zhang J, Cui L, et al. Genetic architecture of fatty acid composition in the longissimus dorsi muscle revealed by genome-wide association studies on diverse pig populations. Genet Sel Evol 2016;48:5. https://doi.org/10.1186/s12711-016-0184-2
  20. Uemoto Y, Soma Y, Sato S, et al. Genome-wide mapping for fatty acid composition and melting point of fat in a purebred Duroc pig population. Anim Genet 2012;43:27-34. https://doi.org/10.1111/j.1365-2052.2011.02218.x
  21. Nii M, Hayashi T, Tani F, et al. Quantitative trait loci mapping for fatty acid composition traits in perirenal and back fat using a Japanese wild boar ${\times}$ Large White intercross. Anim Genet 2006;37:342-47. https://doi.org/10.1111/j.1365-2052.2006.01485.x
  22. Munoz G, Alves E, Fernandez A, et al. QTL detection on porcine chromosome 12 for fatty-acid composition and association analyses of the fatty acid synthase, gastric inhibitory polypeptide and acetylcoenzyme A carboxylase alpha genes. Anim Genet 2007;38:639-46. https://doi.org/10.1111/j.1365-2052.2007.01668.x
  23. Uemoto Y, Sato S, Ohnishi C, et al. The effects of single and epistatic quantitative trait loci for fatty acid composition in a Meishan ${\times}$ Duroc crossbred population. J Anim Sci 2009;87:3470-6. https://doi.org/10.2527/jas.2009-1917
  24. Quintanilla R, Pena RN, Gallardo D, et al. Porcine intramuscular fat content and composition are regulated by QTLs with muscle-specific effects. J Anim Sci 2011;89:2963-71. https://doi.org/10.2527/jas.2011-3974

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