한국균학회지 (The Korean Journal of Mycology)

  • 제45권3호
  • /
  • Pages.234-240
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  • 2017
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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충남 서천군 갈대밭 주변 토양에서 야생효모의 분리 및 미기록종 특성

Isolation of Wild Yeasts from Soils of Reed Fields in Seocheon-gun County, Chungcheongnam-do, South Korea, and Characterization of Unrecorded Yeasts

  • 한상민 (배재대학교 바이오.의생명공학과) ;
  • 이상엽 (배재대학교 바이오.의생명공학과) ;
  • 이향범 (전남대학교 응용생명공학부) ;
  • 이종수 (배재대학교 바이오.의생명공학과)
  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Sang-Yeop (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Hyang-Burm (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 투고 : 2017.08.11
  • 심사 : 2017.08.24
  • 발행 : 2017.09.01
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초록

충남 서천군 한산면 신성리 금강하구 갈대밭 토양의 야생효모 분포특성을 알아보기 위하여 갈대밭 토양 20점을 채취하여 야생효모들을 분리하여 총 11종 20균주의 야생효모를 동정하였다. Candida속의 효모가 10균주로 가장 많이 분리되었고, 그 중 Candida subhashii 균이 6균주로 가장 많았다. 분리된 균주 중에서 Candida tropicalis (Y5-1,Y5-2, Y6-1), Candida subhashii (J7-1, J7-3, J8-1, Y9-1, Y9-2, Y9-3), Lachancea thermotolerans (MJS 007, MJS 019) 균주 등 12균주들이 알코올 발효능을 보였다. Bullera japonica YJ10-1, C. subhashii J7-1, Kluyveromyces yarrowii YJ11-1, Ustilago shanxiensis Y10-1등 4균주들이 국내 미기록 효모 균주들로 최종 선별되었다. 이들 국내 미기록 균주들은 구형으로 Candida subhashii J7-1만이 자낭포자와 의균사를 형성하였다. Kluyveromyces yarrowii YJ11-1은 비타민을 함유하지 않은 배지에서도 생육하였고 Candida subhashii J7-1는 YM과 YPD 배지 및 5% NaCl을 함유한 YPD 배지에서 생장하지 않았다.

The goal of this study was to isolate wild yeasts from reed fields in the Seocheon-gun county in Chungcheongnam-do, South Korea. Molecular analysis of the D1/D2 domain of the 26S rDNA of the yeasts isolated from soil samples was performed using Basic Local Alignment Search Tool (BLAST). In total, 20 yeast strains from 11 species were isolated from 20 different soil samples. Candida species were observed to be the most common yeasts. Of these 20 strains, Candida subhashii (6 strains), Candida tropicalis (3 strains), Candida boleticola MS008, and Lachancea thermotolerans (2 strains) were identified as alcohol-fermenting yeasts. Further, Bullera japonica YJ10-1, Candida subhashii J7-1, Kluyveromyces yarrowii YJ11-1, and Ustilago shanxiensis Y10-1 were newly recorded yeast strains in Korea, and therefore, their microbiological characteristics were investigated further. All of these unrecorded yeast strains had oval cells; only Candida subhashii J7-1 formed ascospores and pseudomycelia. Kluyveromyces yarrowii YJ11-1 grew in vitamin-free medium, and all of these strains, except Candida subhashii J7-1, grew in 5% NaCl-containing YPD broth.

키워드

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