Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Inhibition of the DevSR Two-Component System by Overexpression of Mycobacterium tuberculosis PknB in Mycobacterium smegmatis

  • Bae, Hyun-Jung (Department of Microbiology, Pusan National University) ;
  • Lee, Ha-Na (Department of Microbiology, Pusan National University) ;
  • Baek, Mi-Na (Department of Microbiology, Pusan National University) ;
  • Park, Eun-Jin (Department of Microbiology, Pusan National University) ;
  • Eom, Chi-Yong (Korea Basic Science Institute) ;
  • Ko, In-Jeong (Korea Science Academy of KAIST) ;
  • Kang, Ho-Young (Department of Microbiology, Pusan National University) ;
  • Oh, Jeong-Il (Department of Microbiology, Pusan National University)
  • Received : 2017.05.11
  • Accepted : 2017.07.24
  • Published : 2017.09.30
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Abstract

The DevSR (DosSR) two-component system, which is a major regulatory system involved in oxygen sensing in mycobacteria, plays an important role in hypoxic induction of many genes in mycobacteria. We demonstrated that overexpression of the kinase domain of Mycobacterium tuberculosis (Mtb) PknB inhibited transcriptional activity of the DevR response regulator in Mycobacterium smegmatis and that this inhibitory effect was exerted through phosphorylation of DevR on Thr180 within its DNA-binding domain. Moreover, the purified kinase domain of Mtb PknB significantly phosphorylated RegX3, NarL, KdpE, TrcR, DosR, and MtrA response regulators of Mtb that contain the Thr residues corresponding to Thr180 of DevR in their DNA-binding domains, implying that transcriptional activities of these response regulators might also be inhibited when the kinase domain of PknB is overexpressed.

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References

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