Molecules and Cells

  • 제40권10호
  • /
  • Pages.737-751
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  • 2017
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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GSK-J4-Mediated Transcriptomic Alterations in Differentiating Embryoid Bodies

  • Mandal, Chanchal (Department of Molecular and Life Science, Hanyang University) ;
  • Kim, Sun Hwa (Department of Molecular and Life Science, Hanyang University) ;
  • Kang, Sung Chul (Department of Molecular and Life Science, Hanyang University) ;
  • Chai, Jin Choul (Department of Molecular and Life Science, Hanyang University) ;
  • Lee, Young Seek (Department of Molecular and Life Science, Hanyang University) ;
  • Jung, Kyoung Hwa (Institute of Natural Science and Technology, Hanyang University) ;
  • Chai, Young Gyu (Department of Molecular and Life Science, Hanyang University)
  • 투고 : 2017.05.09
  • 심사 : 2017.08.20
  • 발행 : 2017.10.31
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초록

Histone-modifying enzymes are key players in the field of cellular differentiation. Here, we used GSK-J4 to profile important target genes that are responsible for neural differentiation. Embryoid bodies were treated with retinoic acid ($10{\mu}M$) to induce neural differentiation in the presence or absence of GSK-J4. To profile GSKJ4-target genes, we performed RNA sequencing for both normal and demethylase-inhibited cells. A total of 47 and 58 genes were up- and down-regulated, respectively, after GSK-J4 exposure at a log2-fold-change cut-off value of 1.2 (p-value < 0.05). Functional annotations of all of the differentially expressed genes revealed that a significant number of genes were associated with the suppression of cellular proliferation, cell cycle progression and induction of cell death. We also identified an enrichment of potent motifs in selected genes that were differentially expressed. Additionally, we listed upstream transcriptional regulators of all of the differentially expressed genes. Our data indicate that GSK-J4 affects cellular biology by inhibiting cellular proliferation through cell cycle suppression and induction of cell death. These findings will expand the current understanding of the biology of histone-modifying enzymes, thereby promoting further investigations to elucidate the underlying mechanisms.

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참고문헌

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