Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Establishment of Analytical Method for Propylene Glycol Alginate in Food Products by Size-exclusion Chromatography

Size-exclusion chromatography법에 의한 식품 중 알긴산프로필렌글리콜 분석법 확립

  • Jeong, Eun-Jeong (Department of Food Science & Technology, Chonbuk National University) ;
  • Choi, Yoo-Jeong (Department of Food Science & Technology, Chonbuk National University) ;
  • Lee, Gunyoung (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation) ;
  • Yun, Sang Soon (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation) ;
  • Lim, Ho Soo (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Yong-Suk (Department of Food Science & Technology, Chonbuk National University)
  • 정은정 (전북대학교 식품공학과) ;
  • 최유정 (전북대학교 식품공학과) ;
  • 이근영 (식품의약품안전평가원 첨가물포장과) ;
  • 윤상순 (식품의약품안전평가원 첨가물포장과) ;
  • 임호수 (식품의약품안전평가원 첨가물포장과) ;
  • 김용석 (전북대학교 식품공학과)
  • Received : 2017.08.17
  • Accepted : 2017.09.05
  • Published : 2017.10.30
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Abstract

An analytical method for determination of propylene glycol alginate (PGA) in food products was developed by HPLC-size-exclusion chromatography. The GF-7M HQ column and LT-ELSD detector were determined by considering the instrumental analysis conditions for PGA analysis. The pretreatment method for the analysis of PGA was suitable for 3 hr extraction at $20^{\circ}C$ and 150 rpm according to the extraction temperature. Linearity ($R^2$) for the analysis of PGA was 0.9873 at calibration curve range of 300, 500, 700, 1,000, and 1,500 mg/kg (5 points). The limit of detection and limit of quantification of PGA on HPLC system was 171.43 and 519.50 mg/kg, respectively. The accuracy and coefficient of variation obtained by size-exclusion chromatography were 86.1~110.4% and 4.1~13.5%, respectively. By applying the HPLC-size-exclusion chromatography system, it was possible to analyze the contents of PGA in 134 different types of food products.

HPLC-size-exclusion chromatography에 의해 가공식품에서 알긴산프로필렌글리콜의 함량을 분석하는 방법이 개발되었다. 알긴산프로필렌글리콜을 분석하기 위해 GF-7M HQ column과 LT-ELSD detector가 선정되었다. 알긴산프로필렌글리콜 분석을 위한 전처리 조건으로는 $20^{\circ}C$에서 150 rpm으로 3시간 동안 추출하는 방법이 선정되었다. 알긴산프로필렌글리콜을 5 농도(300, 500, 700, 1,000, and 1,500 mg/kg) 범위에서 검량선을 작성한 결과 직선성($R^2$)은 0.9873으로 측정되었다. HPLC system에 의한 알긴산프로필렌글리콜 분석시 검출한계(LOD) 및 정량한계(LOQ)는 각각 171.43 mg/kg 및 519.50 mg/kg이었다. Size-exclusion chromatography에 의해 얻은 회수율 및 변동 계수(coefficient of variation)는 각각 86.1~110.4% 및 4.1~13.5%이었다. 본 연구에서 개발된 HPLC-size-exclusion chromatography system을 적용하여 134 품목의 가공식품에서 알긴산프로필렌글리콜 함량을 분석하였다. 이 결과들은 이 방법이 가공식품에서 알긴산프로필렌글리콜 함량을 분석하는데 적용할 수 있는 방법이라는 것을 나타낸다.

Keywords

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