Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Plant Regeneration via Adventitious Shoot Formation in Platycodon grandiflorum (Jacq. A. DC.)

도라지 (Platycodon grandiflorum (Jacq.) A. DC.) 부정아 형성을 통한 식물체 재분화

  • Kim, Ju Young (Department of Oriental Pharmaceutical Development, Medicinal Plant Transformation Center, Nambu University) ;
  • Na, Hyun Sun (Department of Oriental Pharmaceutical Development, Medicinal Plant Transformation Center, Nambu University) ;
  • Choi, Pil Son (Department of Oriental Pharmaceutical Development, Medicinal Plant Transformation Center, Nambu University)
  • 김주영 (남부대학교 한방제약개발학과) ;
  • 나현선 (남부대학교 한방제약개발학과) ;
  • 최필선 (남부대학교 한방제약개발학과)
  • Received : 2017.05.22
  • Accepted : 2017.07.13
  • Published : 2017.09.30
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Abstract

To investigate optimal conditions for plant regeneration in Platycodon grandiflorum (Jacq. A. DC.).Both leaf and hypocotyl explants were cultured on Murashige& Skoog's (MS) medium supplemented with combinations of 0.1, 0.5, 1.0, or 2.0 mg/L cytokinins (BA and kinetin) and 1.0 mg/L 2,4-D for 6 weeks, respectively. According to the type of explant, the total shoot organogenesis (56.38%) in leaf explants was higher than in hypocotyls (28.20%). In comparison with kinetin and BA for the plant regeneration, the frequency (70.38%) of leaf explants was higher in combination with kinetin and 2,4-D than of BA with 2,4-D (42.38%), whereas the frequency (35.56%) of hypocotyls explants was higher in BA combination than kinetin combination (20.83%). Thehighest frequency (94.20%) was observed from the cultures of leaf explants on the MS medium supplemented with 1.0 mg/L kinetin and 1.0 mg/L 2,4-D. Upon transfer onto 1/2 MS basal medium containing 3% sucrose, shoots developed into plantlets with roots, and were well grown in soil in the greenhouse. These results lead us to speculate that the optimization of culture conditions was responsible for the mass propagation from in vitro cultures of Platycodon grandiflorum (Jacq. A. DC.).

도라지(Platycodon grandiflorum (Jacq. A. DC.)에서 신초발생에 대한 최적의 호르몬 조건을 조사하기 위하여 잎 및 배축절편을 1.0 mg/L 2,4-D와 0.1, 0.5, 1.0, 2.0 mg/L BA 또는 kinetin과 각각 조합 첨가한 MS 기본배지에 6주 동안 명 배양 하였다. 배양절편에 따라 신초발생율은 잎 절편에서 56.38%로 배축절편(28.20%)보다 높았다. Kinetin과 BA를 2,4-D와 조합첨가 하였을 때 kinetin의 경우 잎에서 70.38%로 BA (42.38%)보다 더 효과적이었으며, 반면 BA의 경우 배축에서는 35.56%로 kinetin (20.83%)보다 더 높은 빈도를 보였다. 가장 높은 신초형성 빈도(94.20%)는 1.0 mg/L 2,4-D와 1.0 mg/L kinetin이 조합첨가된 MS 배지에서 잎 절편을 배양하였을 때 얻을 수 있었다. 대부분의 신초는 1/2 MS기본배지에서 뿌리를 유도한 후 토양에서 완전한 식물체를 얻을 수 있었다. 이러한 결과는 최적의 기내배양 조건을 통해 도라지의 대량생산 가능성을 보여주었다.

Keywords

References

  1. Barwale UB, Meyer MM, Widholm JM (1986) Screening of Glycine max and Glycine soja genotypes for multiple shoot formation at the cotyledonary node. Theor. Appl. Genet. 72:423-428 https://doi.org/10.1007/BF00288582
  2. Cho DY, Lee EK, Soh WY (1998) Plant regeneration from somatic embryo with structural diversity from leaf explants cultures of Osteicum koreanum Kitagawa. Kor J Plant Tiss Cult 25:51-56
  3. Cho HJ, Widholm JM (2002). Improved shoot regeneration protocol for hairy roots of the legume Astragalus sinicus. Plant Cell, Tiss Org Cult 69(3):259-269 https://doi.org/10.1023/A:1015624316573
  4. Choi PS, Komatsuda T, Kim MH, Choi KM, Choi DW, Liu JR (2002) Screening of soybean recombinant inbred lines for high competence somatic embryogenesis. Kor J Plant Biotechnol 29:135-138 https://doi.org/10.5010/JPB.2002.29.2.135
  5. Choi SR, Kim MJ, Eun JS, Ahn MS, Lim HS, Ryu J, You DH (2004) Effects of membrane filter and sucrose concentrations on the growth of balloon flower (Platycodon geandiflorum A. DC.) plantlets in vitro. Kor J Plant Biotechnol 31:209-217 https://doi.org/10.5010/JPB.2004.31.3.209
  6. Chung JH, Cho SH (2002) Tissue cultures of Platycodon grandiflour. J Agricult Life Sci 36:9-18
  7. Eun JS, Kim YS, Kim YH (2000) Effects of light emitting diodes on growth and morphogenesis of in vitro seedling in Platycodon geandiflorum. Kor J Plant Tiss Cult 27:71-75
  8. Erisen S, Yorgancilar M, Atalay E, Babaoglu M, Duran A (2010.) Callus induction and plant regeneration of endemic Astragalus nezaketae in Turkey. Electron of Biotechnol. DOI: 10.2225/vol13-issue6-fulltex-3
  9. Gill R, Saxena PK (1992) Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (Arachis hypogaea): promotive role of thidiazuron. Can J Bot 70:1186-1192 https://doi.org/10.1139/b92-147
  10. Han C, Lee Y (1976) Induction of plantlets in the anther culture of Platycodon grandiflorum. Kor J. Plant Tiss Cult 4:1-3
  11. Han EH, Son YW, Kim MB, Shin YW, Cho YS, Lee SW (2014) Establishment of tissue culture and acclimation of white ballon flower (Platycodon grandiflorum DC. cv. Jangback) for the eaising of in vitro propagated seedlings. J Plant Biotechnol. 41:134-139 https://doi.org/10.5010/JPB.2014.41.3.134
  12. Hou SW, Jia JF (2004) High frequency plant regeneration from Astragalus melilotoides hypocotyl and stem explants via somatic embryogenesis and organogenesis. Plant Cell Tiss Org Cult 79(1):95-100 https://doi.org/10.1023/B:TICU.0000049445.69802.50
  13. Kasumi M, Suzuki K, Gonai T, Nog M, Yamafa T, Takatsu Y (2006) Adventitious buf formation and plant regeneration from ovary explants in balloon flower (Platycodon geandiflorum). J Ja Soc Hort Sci 75:284-286 https://doi.org/10.2503/jjshs.75.284
  14. Kim SW, Liu JR (1999) Somatic embryogenesis and plant regeneration in zygotic embryo cultures of balloon flower. Plant Cell Tiss Org Cult 58:227-230. https://doi.org/10.1023/A:1006338024745
  15. Ko JA (1999) Effect of low temperature pretreatment on pollen dimorphism and embryo formation in anther culture of Platycodon grandiflorum. Kor J Plant Tiss Cult 26:149-156
  16. Ko JA, Kim YS, Kim MJ, Eun JS (1993) Somatic embryogenesis and plant regeneration from leaf and stem culture of Platycodon grandiflorum. Kor J. Breeding 25:337-343
  17. Lee BK, Ko JA, Kim YS, Eun JS (1993) Embryogenesis and plnat regeneration in anther cultures of Platycodon grandiflorum. Kor J Plant Tiss Cult 20:153-157
  18. Lee SW, Son YW, Shin YW, Cho YS (2014) The current status in Korea and future perspectives of ballon flower (Platycodon grandiflorum) in Gyeongsangnam-do. Kor J Int Agric 26:68-72 https://doi.org/10.12719/KSIA.2014.26.1.68
  19. Malik KA, Saxena PK (1992) Regeneration in Phaseoulus vulgaris seedling by $N^6$-benzylaminopurine and thidiazuron. Planta 186:384-389
  20. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473-479 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  21. Park MS, Choi PS (2015) Plant regeneration from hypocotyl explants of Astragalus sinicus L. J Plant Biotechnol 42:396-400 https://doi.org/10.5010/JPB.2015.42.4.396
  22. Soh WY, Cho DY, Lee EK (1996) Multicotyledonary structure of somatic embryo formed cell cultures of Daucus carota L. Korean J Bot 39:71-79
  23. Turgut-Kara N, ARI S (2008) In vitro plant regeneration from embryogenic cell suspension culture of Astragalus chrysochlorus (Leguminoseae). Afr J Biotechnol 7:1250-1255
  24. Wright MS, Koehler SM, Hinchee MA, Carnes MG (1986) Plant regeneration by organogenesis in Glycine max. Plant Cell Rep 5:150-154 https://doi.org/10.1007/BF00269257