Journal of Plant Biotechnology

  • 제44권3호
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  • Pages.287-295
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  • 2017
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Overexpression of the Small Heat Shock Protein, PtsHSP19.3 from Marine Red Algae, Pyropia tenera (Bangiales, Rhodophyta) Enhances Abiotic Stress Tolerance in Chlamydomonas

  • Jin, Yujin (Department of Biology Education and Kumho Life Science Laboratory, Chonnam National University) ;
  • Yang, Sungwhan (Department of Biology Education and Kumho Life Science Laboratory, Chonnam National University) ;
  • Im, Sungoh (Department of Biology Education and Kumho Life Science Laboratory, Chonnam National University) ;
  • Jeong, Won-Joong (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, EunJeong (Seaweed Research Center, National Fisheries Research and Development Institute) ;
  • Choi, Dong-Woog (Department of Biology Education and Kumho Life Science Laboratory, Chonnam National University)
  • 투고 : 2017.06.21
  • 심사 : 2017.08.22
  • 발행 : 2017.09.30
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초록

Water temperature is one of the major factors that impacts the growth and life cycle of Pyropia tenera, one of the most valuable and cultivated marine red algae belonging to Bangiales (Rhodophytes). We analyzed transcriptome from gametophyte of P. tenera under normal and high temperature conditions, and identified four small heat shock proteins (sHSPs). They have no significant amino acid sequence homology with known proteins in public databases except PhsHSP22 from Pyropia haitanensis. PtsHSP19.3 gene responded to high temperature but slightly or not to desiccation, freezing or high salt condition. When the PtsHSP19.3 gene was overexpressed in Chlamydomonas reinhardtii, transformed Chlamydomonas lines revealed much higher growth rate than that of control cells under heat stress condition. Transformed cells also grew well in those of the control cell onto the medium containing high salt or $H_2O_2$. When the PtsHSP19.3 was fused to GFP and introduced into tobacco protoplast, fluorescence was detected at several spots. Results indicate that PtsHSP19.3 may form super-molecular assembles and be involved in tolerance to heat stress.

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참고문헌

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피인용 문헌

  1. Isolation, Morphological Characteristics and Proteomic Profile Analysis of Thermo-tolerant Pyropia yezoensis Mutant in Response to High-temperature Stress pp.2005-7172, 2018, https://doi.org/10.1007/s12601-018-0060-9
  2. PtDRG1, a Desiccation Response Gene from Pyropia tenera (Rhodophyta), Exhibits Chaperone Function and Enhances Abiotic Stress Tolerance vol.20, pp.5, 2018, https://doi.org/10.1007/s10126-018-9828-2
  3. PtsHSP19.6, a small heat-shock protein from the marine red alga Pyropia tenera (Rhodophyta), aggregates into granules and enhances heat tolerance pp.1573-5176, 2019, https://doi.org/10.1007/s10811-018-1728-3
  4. Characterization and expression profiles of small heat shock proteins in the marine red alga Pyropia yezoensis vol.24, pp.1, 2019, https://doi.org/10.1007/s12192-018-00959-9