Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Subacute oral toxicity and bacterial mutagenicity study of Korean Red Ginseng oil

  • Seo, Hwi Won (Laboratory of Fundamental Research, Korea Ginseng Corporation) ;
  • Suh, Jae Hyun (Laboratory of Fundamental Research, Korea Ginseng Corporation) ;
  • So, Seung-Ho (Laboratory of Fundamental Research, Korea Ginseng Corporation) ;
  • Kyung, Jong-Soo (Laboratory of Fundamental Research, Korea Ginseng Corporation) ;
  • Kim, Yong-Soon (Korea Occupational Safety Health Research Institute) ;
  • Han, Chang-Kyun (Laboratory of Fundamental Research, Korea Ginseng Corporation)
  • Received : 2016.10.30
  • Accepted : 2017.01.17
  • Published : 2017.10.15
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Abstract

Background: Red ginseng oil (RGO) is produced by supercritical $CO_2$ extraction of secondary products derived from Korean Red Ginseng extract. As the use of RGO has increased, product safety concerns have become more important. Methods: In the present study, the subacute oral toxicity and bacterial reverse mutagenicity of RGO were evaluated. Sprague-Dawley rats were orally administered with RGO for 28 d by gavage. Daily RGO dose concentrations were 0 mg/kg body weight (bw), 500 mg/kg bw, 1,000 mg/kg bw, or 2,000 mg/kg bw per day. Bacterial reverse mutation tests included five bacterial strains (Escherichia coli WP2 and Salmonella typhimurium TA98, TA100, TA1535, and TA1537), which were used in the presence or absence of metabolic activation. The plated incorporation method for mutation test was used with RGO concentrations ranging from $312.5{\mu}g$ to $5,000{\mu}g$ per plate. Results: The subacute oral toxicity test results did not reveal any marked changes in clinical characteristics. There were no toxicological changes related to RGO administration in hematological and serum biochemical characteristics in either control or treatment animals. Furthermore, no gross or histopathological changes related to RGO treatment were observed. The bacterial reverse mutation test results did not reveal, at any RGO concentration level and in all bacterial strains, any increase in the number of revertant colonies in the RGO treatment group compared to that in the negative control group. Conclusion: The no-observed-adverse-effect level of RGO is greater than 2,000 mg/kg bw and RGO did not induce genotoxicity related to bacterial reverse mutations.

Keywords

References

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