식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
권호 표지
DOI QR코드

DOI QR Code

자단향으로부터 밀 붉은곰팡이병균 Fusarium graminearum에 대한 항진균활성 물질의 분리 및 특성 규명

Isolation and Characterization of Antifungal Metabolites from Pterocarpus santalinus against Fusarium graminearum Causing Fusarium Head Blight on Wheat

  • Kim, Ji-In (Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Ha, Areum (Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Park, Ae Ran (Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, Jin-Cheol (Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University)
  • 투고 : 2017.08.25
  • 심사 : 2017.09.05
  • 발행 : 2017.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

붉은곰팡이병은 주로 Fusarium graminearum에 의해 발생하며 전세계적으로 주요 곡물에 치명적인 피해를 주는 식물병이다. 관행농가에서는 일반적으로 붉은곰팡이병 방제에 합성살균제를 사용하고 있지만 장기적인 합성살균제의 사용으로 인하여 환경 오염 문제와 인간의 건강을 위협하는 문제가 야기되었다. 때문에 천연물살균제를 포함한 친환경 생물농약 개발에 대한 관심이 고조되고 있는 실정이다. 본 연구에서는 100종의 식물추출물을 이용하여 F. graminearum에 항진균 활성을 갖는 식물 추출물을 탐색하였다. 그 결과 자단 심재부인 자단향의 조추출물이 가장 강한 항진균활성을 보였으며, GC-MS 분석을 통하여 자단향으로부터 ${\alpha}$-cedrol과 widdrol로 동정된 두개의 항진균 활성물질을 분리하였다. ${\alpha}$-Cedrol과 widdrol의 F. graminearum 포자발아 억제활성 최소저해농도는 각각 31.25 mg/l과 62.5 mg/l였으며, 이 두 물질은 F. graminearum 이외에도 다양한 식물병원균의 균사 생육을 저해하였다. 더욱이 자단향 추출물을 분말 수화제로 제형화하여 처리한 결과, 밀 붉은 곰팡이병에 대한 방제효과는 유효성분 농도가 높을수록 효과적인 방제가를 보였으며, 250배 희석처리 시 2,000배의 상용농도로 희석한 합성살균제의 방제가(92.6%)와 통계적으로 유사한 87.2%의 병방제가를 보였다. 본 연구결과는 붉은곰팡이병방제를 위한 효과적인 생물살균제로 자단향 추출물이 활용 가능하다는 것을 제시하고 있다.

Fusarium head bight (FHB) is a devastating disease on major cereal crops worldwide which causes primarily by Fusarium graminearum. Synthetic fungicides are generally used in conventional agriculture to control FHB. Their prolonged usage has led to environmental issues and human health problems. This has prompted interest in developing environmentally friendly biofungicides, including botanical fungicides. In this study, a total 100 plant extracts were tested for antifungal activity against F. graminearum. The crude extract of Pterocarpus santalinus heartwood showed the strongest antifungal activity and contained two antifungal metabolites which were identified as ${\alpha}$-cedrol and widdrol by GC-MS analysis. ${\alpha}$-Cedrol and widdrol isolated from P. santalinus heartwood extract had 31.25 mg/l and 125 mg/l of minimal inhibitory concentration against the spore germination of F. graminearum, and also showed broad spectrum antifungal activities against various plant pathogens. In addition, the wettable powder type formulation of heartwood extract of P. santalinus decreased FHB incidence in dose-dependent manner and suppressed the development of FHB with control values of 87.2% at 250-fold dilution, similar to that of chemical fungicide (92.6% at 2,000-fold dilution). This study suggests that the heartwood extract of P. santalinus could be used as an effective biofungicide for the control of FHB.

키워드

참고문헌

  1. Arunakumara, K. K. I. U., Walpola, B. C., Subasinghe, S. and Yoon, M.-H. 2011. Pterocarpus santalinus Linn. f. (Rath handun): a review of its botany, uses, phytochemistry and pharmacology. J. Korean Soc. Appl. Biol. Chem. 54: 495-500.
  2. Bondalapati, K. and Stein, J. 2010. Validation of barley DON risk prediction model. In: Proc 2010 National Fusarium Head Blight Forum, eds. by S. Canty, A. Clark, A. Anderson-Scully, D. Ellis and D. Van Sanford, pp. 71-73. Milwaukee, WI.
  3. Bowden, R. L. and Leslie, J. F. 1999. Sexual recombination in Gibberella zeae. Phytopathology 89: 182-188. https://doi.org/10.1094/PHYTO.1999.89.2.182
  4. Bradley, C. A., Adee, E. A., Ebelhar, S. A, Grybauskas, A. P., Hollingsworth, C. R., Kirk, W. W., McMullen, M. P., Milus, E. A., Osborne, L. E., Ruden, K. R. and Young, B. G. 2009. Application timings of Caramba and Prosaro foliar fungicides for management of FHB and DON. In: Proc 2009 National Fusarium Head Blight Forum, eds. by S. Canty, A. Clark, J. Mundell, E. Walton, D. Ellis and D. Van Sanford, 34 pp. Orlando, FL.
  5. Bradley, C., Adee, E., Ebelhar, S., Dill-Macky, R., Wiersma, J., Grybauskas, A., Krik, W., McMullen, M., Halley, S. and Milus, E. 2010. Multistate uniform fungicide evaluations for control of Fusarium head blight and associated mycotoxins. In: Proc 2010 National Fusarium Head Blight Forum, eds. by S. Canty, A. Clark, A. Anderson-Scully, D. Ellis and D. Van Sanford, 74 pp. Milwaukee, WI.
  6. Cappellini, R. and Peterson, J. 1965. Macroconidium formation in submerged cultures by a non-sporulating strain of Gibberella zeae. Mycologia 57: 962-966. https://doi.org/10.2307/3756895
  7. Chung, H. S. 1975. Cereal scab causing mycotoxicoses in Korea and present status of mycotoxin researches. Kor. J. Mycol. 3: 31-36.
  8. Copping, L. G. and Duke, S. O. 2007. Natural products that have been used commercially as crop protection agents. Pest Manag. Sci. 63: 524-554. https://doi.org/10.1002/ps.1378
  9. Copping, L. G. and Menn, J. J. 2000. Biopesticides: a review of their action, applications and efficacy. Pest Manag. Sci. 56: 651-676. https://doi.org/10.1002/1526-4998(200008)56:8<651::AID-PS201>3.0.CO;2-U
  10. Delye, C., Bousset, L. and Corio-Costet, M. F. 1998. PCR cloning and detection of point mutations in the eburicol 14a-demethylase (CYP51) gene from Erysiphe graminis f. sp. hordei, a "recalcitrant" fungus. Curr. Genet. 34: 399-403. https://doi.org/10.1007/s002940050413
  11. Desjardins, A. E. 2006. Fusarium Mycotoxins: Chemistry, Geneticsand Biology. APS Press, St. Paul, MN.
  12. El-Sawi, S. A., Motawae, H. M. and Ali, A. M. 2007. Chemical composition, cytotoxic activity and antimicrobial activity of essential oils of leaves and berries of Juniperus phoenicea L. grown in Egypt. Afr. J. Tradit. Complement. Altern. Med. 4: 417-426.
  13. Goswami, R. S. and Kistler, H. C. 2004. Heading for disaster: Fusarium graminearum on cereal crops. Mol. Plant Pathol. 5: 515-525. https://doi.org/10.1111/j.1364-3703.2004.00252.x
  14. Guleria, S., Kumar, A. and Tiku, A. K. 2008. Chemical composition and fungitoxic activity of essential oil of Thuja orientalis L. grown in the north-western Himalaya. Z. Naturforsch. C 63: 211-214.
  15. Ibanez-Vea, M., Lizarraga, E., Gonzalez-Penas, E. and Cerain, A. L. 2011. Co-occurrence of type-A and type-B trichothecenes in barley from a northern region of Spain. Food Control 25: 81-88.
  16. Ismail, A., Lamia, H., Mohsen, H., Samia, G. and Bassem, J. 2013. Chemical composition, bio-herbicidal and antifungal activities of essential oils isolated from Tunisian common cypress (Cupressus sempervirens L.). J. Med. Plants Res. 7: 1070-1080.
  17. Jin, S., Yun, H. J., Jeong, H. Y., Oh, Y. N., Park, H. J., Yun, S.-G., Kim, B. W. and Kwon, H. J. 2015. Widdrol, a sesquiterpene isolated from Juniperus chinensis, inhibits angiogenesis by targeting vascular endothelial growth factor receptor 2 signaling. Oncol. Rep. 34: 1178-1184. https://doi.org/10.3892/or.2015.4075
  18. Jyothi Chaitanya, P., Chandrashekar, R. and Lakshmi Bhavani, N. 2014. Assessment of histochemical, phytochemical and antimicrobial activity of Pterocarpus santalinus L wood extracts. European J. Biomed. Pharm. Sci. 1: 230-241.
  19. Kim, J.-I., Kim, K., Park, A. R., Choi, G. J., Park, H. W., Kim, I. S. and Kim, J.-C. 2016. Biological control of Fusarium head blight on wheat by polyacetylenes derived from Cirsium japonicum roots. Res. Plant Dis. 22:145-151. (In Korean) https://doi.org/10.5423/RPD.2016.22.3.145
  20. Kondeti, V. K., Badri, K. R., Maddirala, D. R., Thur, S. K. M., Fatima, S. S., Kasetti, R. B. and Rao, C. A. 2010. Effect of Pterocarpus santalinus bark, on blood glucose, serum lipids, plasma insulin and hepatic carbohydrate metabolic enzymes in streptozotocin-induced diabetic rats. Food Chem. Toxicol. 48: 1281-1287. https://doi.org/10.1016/j.fct.2010.02.023
  21. Kusuma, I. W., Itoh, K. and Tachibana, S. 2005. Antifungal activities against plant pathogenic fungi of flavonoids isolated from Amboyna wood. Pak. J. Biol. Sci. 8: 136-140. https://doi.org/10.3923/pjbs.2005.136.140
  22. Kwon, H., Hong, Y., Kim, K., Han, C., Cho, S., Choi, J. and Kim, B.-W. 2006. Methanolic extract of Pterocarpus santalinus induces apoptosis in HeLa cells. J. Ethnopharmacol. 105: 229-234. https://doi.org/10.1016/j.jep.2005.10.025
  23. Le Dang, Q., Lim, C.-H. and Kim, J.-C. 2012. Current status of botanical pesticides for crop protection. Res. Plant Dis. 18: 175-185. https://doi.org/10.5423/RPD.2012.18.3.175
  24. Leroux, P., Albertini, C., Gautier, A., Gredt, M. and Walker, A. S. 2007. Mutations in the CYP51 gene correlated with changes in sensitivity to sterol 14a-demethylation inhibitors in field isolates of Mycosphaerella graminicola. Pest Manag. Sci. 63: 688-698. https://doi.org/10.1002/ps.1390
  25. Leslie, J. F. and Summerell, B. A. 2008. The Fusarium Laboratory Manual. John Wiley & Sons.
  26. Luo, C. X. and Schnabel, G. 2008. The cytochrome P450 lanosterol 14a-demethylase gene is a demethylation inhibitor fungicide resistance determinant in Monilinia fructicola field isolates from Georgia. Appl. Environ. Microbiol. 74: 359-366. https://doi.org/10.1128/AEM.02159-07
  27. Manjunatha, B. 2006. Hepatoprotective activity of Pterocarpus santalinus L. f., an endangered medicinal plant. Indian. J. Pharmacol. 38: 25-28. https://doi.org/10.4103/0253-7613.19848
  28. McMullen, M., Jones, R. and Gallenberg, D. 1997. Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis. 81: 1340-1348. https://doi.org/10.1094/PDIS.1997.81.12.1340
  29. McMullen, M., Bergstrom, G., De Wolf, E., Dill-Macky, R., Hershman, D., Shaner, G. and Van Sanford, D. 2012. A unified effort to fight an enemy of wheat and barley: Fusarium head blight. Plant Dis. 96: 1712-1728. https://doi.org/10.1094/PDIS-03-12-0291-FE
  30. Nagaraju, N., Prasad, M., Gopalakrishna, G. and Rao, K. 1991. Blood sugar lowering effect of Pterocarpus santalinus (Red Sanders) wood extract in different rat models. Int. J. Pharmacogn. 29: 141-144. https://doi.org/10.3109/13880209109082866
  31. Narayan, S., Devi, R., Srinivasan, P. and Devi, C. 2005. Pterocarpus santalinus: a traditional herbal drug as a protectant against ibuprofen induced gastric ulcers. Phytother. Res. 19: 958-962. https://doi.org/10.1002/ptr.1764
  32. Nunez, Y. O., Salabarria, I. S., Collado, I. G. and Hernandez-Galan, R. 2006. The antifungal activity of widdrol and its biotransformation by Colletotrichum gloeosporioides (penz.) Penz. & Sacc. and Botrytis cinerea Pers.: Fr. J. Agric. Food Chem. 54: 7517-7521. https://doi.org/10.1021/jf061436m
  33. Park, J.-M., Shin, S.-H., Kang, C.-S., Kim, K.-H., Cho, K.-M., Choi, J.-S., Kim, H.-M. and Park, J.-C. 2012. Fungicide effects in vitro and in field trials on Fusarium head blight of wheat. Res. Plant Dis. 18: 194-200. (In Korean) https://doi.org/10.5423/RPD.2012.18.3.194
  34. Peraica, M. and Domijan, A.-M. 2001. Contamination of food with mycotoxins and human health. Arh. Hig. Rada Toksikol. 52: 23-35.
  35. Pilotti, C. A., Kondo, R., Shimizu, K. and Sakai, K. 1995. An examination of the anti-fungal components in the heartwood extracts of Pterocarpus indicus. Mokuzai Gakkaishi 41: 593-597.
  36. Ryu, J.-G., Lee, S.-H., Son, S.-W., Lee, S.-H., Nam, Y.-J., Kim, M.-J., Lee, T. and Yun, J.-C. 2011. Natural occurrence of Fusarium head blight and its mycotoxins in 2010-harvested barley and wheat grains in Korea. Res. Plant Dis. 17: 272-279. (In Korean) https://doi.org/10.5423/RPD.2011.17.3.272
  37. Sabine, J. R. 1975. Exposure to an environment containing the aromatic red cedar, Juniperus virginiana: procarcinogenic, enzymeinducing and insecticidal effects. Toxicology 5: 221-235. https://doi.org/10.1016/0300-483X(75)90119-5
  38. Shim, H. G., Noh, T.-H., Lee, D.-K., Song, J.-J. and Oh, I.-S. 2009. Occurrence of rice headblight in Honam area and infectionmechanism. In: Annual Report for 2008, pp. 453-461. National Institute of Crop Science.
  39. Son, S.-W., Nam, Y.-J., Lee, S.-H., Lee, S.-M., Lee, S.-H., Kim, M.-J., Lee, T., Yun, J.-C. and Ryu, J.-G. 2011. Toxigenic fungal contaminants in the 2009-harvested rice and its milling-by products samples collected from rice processing complexes in Korea. Res. Plant Dis. 17: 280-287. (In Korean) https://doi.org/10.5423/RPD.2011.17.3.280
  40. Tumen, I., Eller, F. J., Clausen, C. A. and Teel, J. A. 2013. Antifungal activity of heartwood extracts from three Juniperus species. Bio-Resources 8: 12-20.
  41. Vandita, P., Amin, N., Khyati, P. and Monisha, K. 2013. Effect of phytochemical constituents of Ricinus communis, Pterocarpus santalinus, Terminalia belerica on antibacterial, antifungal and cytotoxic activity. Int. J. Toxicol. Pharmacol. Res. 5: 47-54.
  42. Wu, S.-F., Chang, F.-R., Wang, S.-Y., Hwang, T.-L., Lee, C.-L., Chen, S.-L., Wu, C.-C. and Wu, Y.-C. 2011. Anti-inflammatory and cytotoxic neoflavonoids and benzofurans from Pterocarpus santalinus. J. Nat. Prod. 74: 989-996. https://doi.org/10.1021/np100871g
  43. Yin, Y., Liu, X., Li, B. and Ma, Z. 2009. Characterization of sterol demethylation inhibitor-resistant isolates of Fusarium asiaticum and F. graminearum collected from wheat in China. Phytopathology 99: 487-497. https://doi.org/10.1094/PHYTO-99-5-0487
  44. Yoon, M.-Y., Cha, B. and Kim, J.-C. 2013. Recent trends in studies on botanical fungicides in agriculture. Plant Pathol. J. 29: 1-9. https://doi.org/10.5423/PPJ.RW.05.2012.0072
  45. Yoon, M. Y., Choi, G., Choi, Y., Jang, K., Park, M., Cha, B. and Kim, J. C. 2010. Effect of polyacetylenic acids from Prunella vulgaris on various plant pathogens. Lett. Appl. Microbiol. 51: 511-517. https://doi.org/10.1111/j.1472-765X.2010.02922.x