The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization of the rcsA Gene from Pantoea sp. Strain PPE7 and Its Influence on Extracellular Polysaccharide Production and Virulence on Pleurotus eryngii

  • Kim, Min Keun (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Lee, Sun Mi (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Seuk, Su Won (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Ryu, Jae San (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Kim, Hee Dae (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Kwon, Jin Hyeuk (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Choi, Yong Jo (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Yun, Han Dae (Department of Applied Life Chemistry, College of Agriculture and Life Science, Gyeongsang National University)
  • Received : 2016.08.30
  • Accepted : 2017.02.27
  • Published : 2017.06.01
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Abstract

RcsA is a positive activator of extracellular polysaccharide (EPS) synthesis in the Enterobacteriaceae. The rcsA gene of the soft rot pathogen Pantoea sp. strain PPE7 in Pleurotus eryngii was cloned by PCR amplification, and its role in EPS synthesis and virulence was investigated. The RcsA protein contains 3 highly conserved domains, and the C-terminal end of the open reading frame shared significant amino acid homology to the helix-turn-helix DNA binding motif of bacterial activator proteins. The inactivation of rcsA by insertional mutagenesis created mutants that had decreased production of EPS compared to the wild-type strain and abolished the virulence of Pantoea sp. strain PPE7 in P. eryngii. The Pantoea sp. strain PPE7 rcsA gene was shown to strongly affect the formation of the disease symptoms of a mushroom pathogen and to act as the virulence factor to cause soft rot disease in P. eryngii.

Keywords

References

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