Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Correlation between Biosurfactants and Antifungal Activity of a Biocontrol Bacterium, Bacillus amyloliquefaciens LM11

생물적 방제균 Bacillus amyloliquefaciens LM11의 유래 생물계면활성물질과 항균활성과의 상관관계

  • Kang, Beom Ryong (Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Kim, Yong Hwan (College of Life and Resource Science, Dankook University) ;
  • Nam, Hyo Song (Bio Control Research Center, Jeonnam Bioindustry Foundation) ;
  • Kim, Young Cheol (Institute of Environmentally-Friendly Agriculture, Chonnam National University)
  • 강범용 (전남대학교 친환경농업연구소) ;
  • 김용환 (단국대학교 식량생명공학과) ;
  • 남효송 (전남생물산업진흥원 생물방제연구센터) ;
  • 김영철 (전남대학교 친환경농업연구소)
  • Received : 2017.02.02
  • Accepted : 2017.02.21
  • Published : 2017.06.30
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Abstract

Bacillus amyloliquefaciens LM11 was isolated from the feces of larvae of the rhino beetle and showed strong antifungal activities against various phytopathogenic fungi by producing biosurfactants. In this study, our overall goal was to determine relationship between biosurfactants produced from the LM11 strain and its role in growth inhibition of phytopathogenic fungi. Production and expression levels of B. amyloliquefaciens LM11 biosurfactants were significantly differed depending on growth phases. Transcriptional and biochemical analysis indicated that the biosurfactants of the LM11 strain were greatly enhanced in late log-phase to stationary phase. Inhibitions of phytopathogenic mycelial growth and spore germination were directly correlated (P<0.001, R=0.761) with concentrations of the LM11 cell-free culture filtrates. The minimum inhibitory surface tension of the culture filtrate of the B. amyloliquefaciens LM11 grown in stationary phase to inhibit mycelial growth of the phytopathogenic fungi was 38.5 mN/m (P<0.001, R=0.951-0.977). Our results indicated that the biosurfactants of B. amyloliquefaciens LM11 act as key antifungal metabolites in biocontrol of plant diseases, and measuring surface tension of the cell-free culture fluids can be used as an easy indicator for optimal usage of the biocontrol agents.

장수풍뎅이 유충의 장내세포에서 분리한 Bacillus amyloliquefaciens LM11은 surfactin, iturin, fengycin 같은 biosurfactants lipopeptide를 생산하여 식물병원성 곰팡이의 성장을 강하게 억제하였다. LM11균주 성장단계에 따라 biosurfactant 생산과 surface tension은 상당히 유의한 차이가 있었다. 항균 물질인 surfactin, iturin, fengycin의 생합성 유전자는 정지기에 도달하면서 집중적으로 발현되었고 그 생산량도 높았다. 또한 LM11균주를 제거한 배양 상등액 함량의 농도에 따라 고추 탄저병원균의 포자발아와 높은 부의 상관관계가 있었다(R=0.761, P<0.001). 식물병원성 곰팡이의 균사 생장억제를 위한 최소 surface tension 수준은 38.5 mN/m였다(R=0.951-0.977, P<0.001). 본 연구 결과는 B. amyloliquefaciens LM11의 biosurfactant가 식물병에 대한 생물학적 방제에 중요한 항진균 대사물질로 작용하며, 배양액의 surface tension 측정은 생물학적 방제제의 최적 사용을 위한 기초 지표로 사용될 수 있음을 보여 주었다.

Keywords

References

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