Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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Anti-inflammatory Effect of Cultivated Wild Panax ginseng Extracts at Various Ages in RAW264.6 Macrophages

RAW264.7 대식세포주에서 근령별 산양삼 추출물의 항염증 효과

  • Lee, Geun (Graduate Program in Biomaterials Science & Engineering, Yonsei University) ;
  • Na, Guihwan (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Wooki (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Baik, Mooyeol (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Lee, Hyungjae (Department of Food Engineering, Dankook University) ;
  • Hwang, Jae-Kwan (Graduate Program in Biomaterials Science & Engineering, Yonsei University)
  • 이근 (연세대학교 생물소재공학협동과정) ;
  • 나귀환 (경희대학교 식품생명공학과) ;
  • 김우기 (경희대학교 식품생명공학과) ;
  • 백무열 (경희대학교 식품생명공학과) ;
  • 이형재 (단국대학교 식품공학과) ;
  • 황재관 (연세대학교 생물소재공학협동과정)
  • Received : 2017.05.31
  • Accepted : 2017.06.13
  • Published : 2017.08.31
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Abstract

It is well-known that cultivated wild Panax ginseng has anti-inflammatory effect. However, a comparative study on cultivation period vs biofunctionality is currently lacking. In this study, 70% ethanol extracts of 3-years (yrs)-, 5-yrs-, or 7-yrs-old cultivated wild ginseng were evaluated for their inhibitory effects on RAW264.7 murine macrophages. Specifically, the production of pro-inflammatory cytokines (interleukin-6 [IL-6] and tumor necrosis factor-alpha [TNF-${\alpha}$]), the expression of surface proteins (CD80, CD86, and MHC-II), and the phagocytic properties were investigated. RAW264.7 cells were induced by 500 ng/mL of lipopolysaccharide (LPS) and treated with 0.1, 1, and 10 ppm of samples. LPS-induced IL-6, TNF-${\alpha}$ and surface proteins in all samples were down-regulated in a dose-dependent manner. Both IL-6 and TNF-${\alpha}$ were significantly reduced at 10 ppm of the 7-yrs-old sample compared to 10 ppm of 3-yrs- and 5-yrs-old samples. CD80 and CD86 were also reduced at 10 ppm of all samples, and there was no difference among samples. The phagocytosis has no difference except in 10 ppm of 3 yr-old sample. The results suggest that cultivated wild ginseng extract has anti-inflammatory effect without decreasing phagocytosis.

Keywords

Acknowledgement

Supported by : 농림수산식품기술기획평가원

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