Korean Circulation Journal

  • 제47권5호
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  • Pages.714-726
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  • 2017
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  • 1738-5520(pISSN)
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  • 1738-5555(eISSN)
대한심장학회 (The Korean Society of Cardiology)
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Remote Ischemic Conditioning by Effluent Collected from a Novel Isolated Hindlimb Model Reduces Infarct Size in an Isolated Heart Model

  • Youn, Young Jin (Division of Cardiology, Department of Internal Medicine, Yonsei University Wonju College of Medicine) ;
  • Yoo, Byung-Su (Division of Cardiology, Department of Internal Medicine, Yonsei University Wonju College of Medicine) ;
  • Son, Jung-Woo (Division of Cardiology, Department of Internal Medicine, Yonsei University Wonju College of Medicine) ;
  • Lee, Jun-Won (Division of Cardiology, Department of Internal Medicine, Yonsei University Wonju College of Medicine) ;
  • Ahn, Min-Soo (Division of Cardiology, Department of Internal Medicine, Yonsei University Wonju College of Medicine) ;
  • Ahn, Sung Gyun (Division of Cardiology, Department of Internal Medicine, Yonsei University Wonju College of Medicine) ;
  • Kim, Jang-Young (Division of Cardiology, Department of Internal Medicine, Yonsei University Wonju College of Medicine) ;
  • Lee, Seung-Hwan (Division of Cardiology, Department of Internal Medicine, Yonsei University Wonju College of Medicine) ;
  • Yoon, Junghan (Division of Cardiology, Department of Internal Medicine, Yonsei University Wonju College of Medicine) ;
  • Eom, Young Woo (Cell Therapy and Tissue Engineering Center, Yonsei University Wonju College of Medicine) ;
  • Oh, Ji-Eun (Cell Therapy and Tissue Engineering Center, Yonsei University Wonju College of Medicine) ;
  • Choi, Seong-Kyung (Animal Core, Central Research Laboratory, Yonsei University Wonju College of Medicine)
  • 투고 : 2017.04.26
  • 심사 : 2017.05.22
  • 발행 : 2017.09.30
⟨ 이전 논문 다음 논문 ⟩

초록

Background and Objectives: Experimental protocols for remote ischemic conditioning (RIC) utilize models in which a tourniquet is placed around the hindlimb or effluent is collected from an isolated heart. In analyzing the humoral factors that act as signal transducers in these models, sampled blood can be influenced by systemic responses, while the effluent from an isolated heart might differ from that of the hindlimb. Thus, we designed a new isolated hindlimb model for RIC and tested whether the effluent from this model could affect ischemia/reperfusion (IR) injury and if the reperfusion injury salvage kinase (RISK) and survivor activating factor enhancement (SAFE) pathways are involved in RIC. Materials and Methods: After positioning needles into the right iliac artery and vein of rats, Krebs-Henseleit buffer was perfused using a Langendorff apparatus, and effluent was collected. The RIC protocol consisted of 3 cycles of IR for 5 minutes. In the RIC effluent group, collected effluent was perfused in an isolated heart for 10 minutes before initiating IR injury. Results: Compared with the control group, the infarct area in the RIC effluent group was significantly smaller ($31.2%{\pm}3.8%$ vs. $20.6%{\pm}1.8%$, p<0.050), while phosphorylation of signal transducer and activation of transcription-3 (STAT-3) was significantly increased. However, there was a trend of increased phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 in this group. Conclusion: This is the first study to investigate the effect of effluent from a new isolated hindlimb model after RIC on IR injury in an isolated heart model. The RIC effluent was effective in reducing the IR injury, and the cardioprotective effect was associated with activation of the SAFE pathway.

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참고문헌

  1. Yellon DM, Hausenloy DJ. Myocardial reperfusion injury. N Engl J Med 2007;357:1121-35. https://doi.org/10.1056/NEJMra071667
  2. Murry CE, Jennings RB, Reimer KA. Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium. Circulation 1986;74:1124-36. https://doi.org/10.1161/01.CIR.74.5.1124
  3. Zhao ZQ, Corvera JS, Halkos ME, et al. Inhibition of myocardial injury by ischemic postconditioning during reperfusion: comparison with ischemic preconditioning. Am J Physiol Heart Circ Physiol 2003;285:H579-88. https://doi.org/10.1152/ajpheart.01064.2002
  4. Heusch G, Botker HE, Przyklenk K, Redington A, Yellon D. Remote ischemic conditioning. J Am Coll Cardiol 2015;65:177-95. https://doi.org/10.1016/j.jacc.2014.10.031
  5. Xin P, Zhu W, Li J, et al. Combined local ischemic postconditioning and remote perconditioning recapitulate cardioprotective effects of local ischemic preconditioning. Am J Physiol Heart Circ Physiol 2010;298:H1819-31. https://doi.org/10.1152/ajpheart.01102.2009
  6. Tamareille S, Mateus V, Ghaboura N, et al. RISK and SAFE signaling pathway interactions in remote limb ischemic perconditioning in combination with local ischemic postconditioning. Basic Res Cardiol 2011;106:1329-39. https://doi.org/10.1007/s00395-011-0210-z
  7. Huffman LC, Koch SE, Butler KL. Coronary effluent from a preconditioned heart activates the JAK-STAT pathway and induces cardioprotection in a donor heart. Am J Physiol Heart Circ Physiol 2008;294:H257-62. https://doi.org/10.1152/ajpheart.00769.2007
  8. Breivik L, Helgeland E, Aarnes EK, Mrdalj J, Jonassen AK. Remote postconditioning by humoral factors in effluent from ischemic preconditioned rat hearts is mediated via PI3K/Akt-dependent cell-survival signaling at reperfusion. Basic Res Cardiol 2011;106:135-45. https://doi.org/10.1007/s00395-010-0133-0
  9. Hausenloy DJ, Yellon DM. Survival kinases in ischemic preconditioning and postconditioning. Cardiovasc Res 2006;70:240-53. https://doi.org/10.1016/j.cardiores.2006.01.017
  10. Hausenloy DJ, Yellon DM. New directions for protecting the heart against ischaemia-reperfusion injury: targeting the Reperfusion Injury Salvage Kinase (RISK)-pathway. Cardiovasc Res 2004;61:448-60. https://doi.org/10.1016/j.cardiores.2003.09.024
  11. Lacerda L, Somers S, Opie LH, Lecour S. Ischaemic postconditioning protects against reperfusion injury via the SAFE pathway. Cardiovasc Res 2009;84:201-8. https://doi.org/10.1093/cvr/cvp274
  12. Lecour S. Activation of the protective Survivor Activating Factor Enhancement (SAFE) pathway against reperfusion injury: does it go beyond the RISK pathway? J Mol Cell Cardiol 2009;47:32-40. https://doi.org/10.1016/j.yjmcc.2009.03.019
  13. Skyschally A, Gent S, Amanakis G, Schulte C, Kleinbongard P, Heusch G. Across-species transfer of protection by remote ischemic preconditioning with species-specific myocardial signal transduction by reperfusion injury salvage kinase and survival activating factor enhancement pathways. Circ Res 2015;117:279-88. https://doi.org/10.1161/CIRCRESAHA.117.306878
  14. Heidbreder M, Naumann A, Tempel K, Dominiak P, Dendorfer A. Remote vs. ischaemic preconditioning: the differential role of mitogen-activated protein kinase pathways. Cardiovasc Res 2008;78:108-15. https://doi.org/10.1093/cvr/cvm114
  15. Pickard JM, Davidson SM, Hausenloy DJ, Yellon DM. Co-dependence of the neural and humoral pathways in the mechanism of remote ischemic conditioning. Basic Res Cardiol 2016;111:50. https://doi.org/10.1007/s00395-016-0568-z
  16. Song BW, Hwang HJ, Seung M, Lee MH. Effect of hypoxic paracrine media on calcium-regulatory proteins in infarcted rat myocardium. Korean Circ J 2014;44:16-21. https://doi.org/10.4070/kcj.2014.44.1.16
  17. Li YW, Li YM, Hon Y, et al. AT1 receptor modulator attenuates the hypercholesterolemia-induced impairment of the myocardial ischemic post-conditioning benefits. Korean Circ J 2017;47:182-92. https://doi.org/10.4070/kcj.2015.0295
  18. Ferrera R, Benhabbouche S, Bopassa JC, Li B, Ovize M. One hour reperfusion is enough to assess function and infarct size with TTC staining in Langendorff rat model. Cardiovasc Drugs Ther 2009;23:327-31. https://doi.org/10.1007/s10557-009-6176-5
  19. Przyklenk K, Bauer B, Ovize M, Kloner RA, Whittaker P. Regional ischemic 'preconditioning' protects remote virgin myocardium from subsequent sustained coronary occlusion. Circulation 1993;87:893-9. https://doi.org/10.1161/01.CIR.87.3.893
  20. Dickson EW, Lorbar M, Porcaro WA, et al. Rabbit heart can be "preconditioned" via transfer of coronary effluent. Am J Physiol 1999;277:H2451-7.
  21. Serejo FC, Rodrigues LF Jr, da Silva Tavares KC, de Carvalho AC, Nascimento JH. Cardioprotective properties of humoral factors released from rat hearts subject to ischemic preconditioning. J Cardiovasc Pharmacol 2007;49:214-20. https://doi.org/10.1097/FJC.0b013e3180325ad9
  22. Weinbrenner C, Nelles M, Herzog N, Sarvary L, Strasser RH. Remote preconditioning by infrarenal occlusion of the aorta protects the heart from infarction: a newly identified non-neuronal but PKC-dependent pathway. Cardiovasc Res 2002;55:590-601. https://doi.org/10.1016/S0008-6363(02)00446-7
  23. Heinen NM, Putz VE, Gorgens JI, et al. Cardioprotection by remote ischemic preconditioning exhibits a signaling pattern different from local ischemic preconditioning. Shock 2011;36:45-53. https://doi.org/10.1097/SHK.0b013e31821d8e77
  24. Wong GT, Lu Y, Mei B, Xia Z, Irwin MG. Cardioprotection from remote preconditioning involves spinal opioid receptor activation. Life Sci 2012;91:860-5. https://doi.org/10.1016/j.lfs.2012.08.037
  25. Gross GJ, Baker JE, Moore J, Falck JR, Nithipatikom K. Abdominal surgical incision induces remote preconditioning of trauma (RPCT) via activation of bradykinin receptors (BK2R) and the cytochrome P450 epoxygenase pathway in canine hearts. Cardiovasc Drugs Ther 2011;25:517-22. https://doi.org/10.1007/s10557-011-6321-9
  26. Jones WK, Fan GC, Liao S, et al. Peripheral nociception associated with surgical incision elicits remote nonischemic cardioprotection via neurogenic activation of protein kinase C signaling. Circulation 2009;120:S1-9. https://doi.org/10.1161/CIRCULATIONAHA.108.843938
  27. Gross ER, Hsu AK, Urban TJ, Mochly-Rosen D, Gross GJ. Nociceptive-induced myocardial remote conditioning is mediated by neuronal gamma protein kinase C. Basic Res Cardiol 2013;108:381. https://doi.org/10.1007/s00395-013-0381-x
  28. Sachdeva J, Dai W, Gerczuk PZ, Kloner RA. Combined remote perconditioning and postconditioning failed to attenuate infarct size and contractile dysfunction in a rat model of coronary artery occlusion. J Cardiovasc Pharmacol Ther 2014;19:567-73. https://doi.org/10.1177/1074248413518967

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