Korean Circulation Journal

The Korean Society of Cardiology (대한심장학회)
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Inhibiting Cytochrome C Oxidase Leads to Alleviated Ischemia Reperfusion Injury

  • Yang, Zhaoyun (Department of Anesthesia, Xiang-Ya Second Hospital, Central South University) ;
  • Duan, Zhongxin (Department of Anesthesiology, the Second Affiliated Hospital, University of South China) ;
  • Yu, Tian (Department of Anesthesiology, Zunyi Medical College) ;
  • Xu, Junmei (Department of Anesthesia, Xiang-Ya Second Hospital, Central South University) ;
  • Liu, Lei (Department of Anesthesia, Xiang-Ya Second Hospital, Central South University)
  • Received : 2016.03.28
  • Accepted : 2016.07.07
  • Published : 2017.03.31
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Abstract

Background and Objectives: The overall purpose of this study was to investigate the role of cytochrome C oxidase (CcO) in preventing ischemia reperfusion-induced cardiac injury through gaseous signaling molecule pathways. Materials and Methods: We used CcO inhibitor, potassium cyanide (KCN) to mimic the pre-treatment of gaseous signaling molecules in a global ischemia/reperfusion (IR) injury model in rats. Intracellular reactive oxygen species (ROS) was determined by measuring mitochondrial $H_2O_2$ and mitochondrial complex activity. Results: KCN pre-treatment led to decreased infarction area after IR injury and improved cardiac function. KCN pre-treated group challenged with IR injury was associated with reduced ROS production through inhibition of activity and not downregulation of CcO expression. In addition, KCN pre-treatment was associated with enhanced expression and activity of mitochondrial antioxidase, suggesting the role of CcO in regulating IR injury through oxidative stress. Conclusion: KCN pre-treatment reduced the severity of IR injury. The potential mechanism could be increased endogenous anti-oxidase activity and consequently, the enhanced clearance of ROS.

Keywords

Acknowledgement

Supported by : National Natural Science Foundation of China

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