Korean Journal of Radiology

The Korean Society of Radiology (대한영상의학회)
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Correlation between Intravoxel Incoherent Motion Magnetic Resonance Imaging Derived Metrics and Serum Soluble CD40 Ligand Level in an Embolic Canine Stroke Model

  • Xu, Xiao-Quan (Department of Radiology, The First Affiliated Hospital of Nanjing Medical University) ;
  • Wu, Chen-Jiang (Department of Radiology, The First Affiliated Hospital of Nanjing Medical University) ;
  • Lu, Shan-Shan (Department of Radiology, The First Affiliated Hospital of Nanjing Medical University) ;
  • Gao, Qian-Qian (Department of Radiology, The First Affiliated Hospital of Nanjing Medical University) ;
  • Zu, Qing-Quan (Department of Radiology, The First Affiliated Hospital of Nanjing Medical University) ;
  • Liu, Xing-Long (Department of Radiology, The First Affiliated Hospital of Nanjing Medical University) ;
  • Shi, Hai-Bin (Department of Radiology, The First Affiliated Hospital of Nanjing Medical University) ;
  • Liu, Sheng (Department of Radiology, The First Affiliated Hospital of Nanjing Medical University)
  • Received : 2016.12.07
  • Accepted : 2017.01.22
  • Published : 2017.10.01
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Abstract

Objective: To determine the relationship between intravoxel incoherent motion (IVIM) imaging derived quantitative metrics and serum soluble CD40 ligand (sCD40L) level in an embolic canine stroke model. Materials and Methods: A middle cerebral artery occlusion model was established in 24 beagle dogs. Experimental dogs were divided into low- and high-sCD40L group according to serum sCD40L level at 4.5 hours after establishing the model. IVIM imaging was scanned at 4.5 hours after model establishment using 10 b values ranging from 0 to $900s/mm^2$. Quantitative metrics diffusion coefficient (D), pseudodiffusion coefficient ($D^*$), and perfusion fraction (f) of ischemic lesions were calculated. Quantitative metrics of ischemic lesions were normalized by contralateral hemisphere using the following formula: normalized $D=D_{stroke}/D_{contralateral}$. Differences in IVIM metrics between the low- and high-sCD40L groups were compared using t test. Pearson's correlation analyses were performed to determine the relationship between IVIM metrics and serum sCD40L level. Results: The high-sCD40L group showed significantly lower f and normalized f values than the low-sCD40L group (f, p < 0.001; normalized f, p < 0.001). There was no significant difference in $D^*$, normalized $D^*$, D, or normalized D value between the two groups (All p > 0.05). Both f and normalized f values were negatively correlated with serum sCD40L level (f, r = -0.789, p < 0.001; normalized f, r = -0.823, p < 0.001). However, serum sCD40L level had no significant correlation with $D^*$, normalized $D^*$, D, or normalized D (All p > 0.05). Conclusion: The f value derived from IVIM imaging was negatively correlated with serum sCD40L level. f value might serve as a potential imaging biomarker to assess the formation of microvascular thrombosis in hyperacute period of ischemic stroke.

Keywords

Acknowledgement

Supported by : National Natural Science Foundation of China

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