Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Comparative Evaluation on Sperm Parameter of Transgenic Pigs with General Pigs

형질전환 돼지의 정자와 일반돼지의 정자성상에 대한 비교평가

  • Park, Sang Hyoun (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Lee, Gunsup (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Lee, Joo Yung (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Kim, Kyung Woon (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Byun, Sung-June (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Ock, Sun A (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Hwang, Seongsoo (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Yang, Hyeon (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Woo, Jae-Seok (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • 박상현 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 이건섭 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 이주영 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 김경운 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 변승준 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 옥선아 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 황성수 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 양현 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 우제석 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 오건봉 (농촌진흥청 국립축산과학원 동물바이오공학과)
  • Received : 2017.08.08
  • Accepted : 2017.09.15
  • Published : 2017.09.30
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Abstract

Pig has been known to be one of the most feasible animals as a bioreactor to produce pharmaceuticals in milk and as a mediator in xenotransplantation research. Previously, we generated transgenic pigs for both purposes, which were expressing Factor 8, vWF, hTPA, and hEPO in milk, along with expression of MCP at GalT gene locus ($GalT^{-MCP/-MCP}$) as well as expressing MCP at GalT gene loci with CD73 expression ($GalT^{-MCP/+}/CD73$). In this study, we performed comparative analyses of sperm parameters between wild type male (WT) pig and those transgenic males to examine the effects of transgenes integrated into the pigs on motility, morphology, viability, and acrosome integrity of the spermatozoa. Our results showed that the rates of actively motile spermatozoa of WT, Factor 8, vWF, hTPA, hEPO, $GalT^{-MCP/+}/CD73$, and $GalT^{-MCP/-MCP}$ pigs were 85.0%, 83.3%, 82.5%, 83.3%, 82.5%, 77.5%, and 78.7%, respectively. Whereas, the rates of morphologically normal spermatozoa of WT, Factor 8, vWF, hTPA, hEPO, $GalT^{-MCP/+}/CD73$, and $GalT^{-MCP/-MCP}$ pigs were 90.0%, 80.0%, 80.0%, 83.3%, 85.0%, 91.8%, and 80.8%, respectively. In addition, the viability in spermatozoa of WT, Factor 8, vWF, hTPA, hEPO, $GalT^{-MCP/+}/CD73$, and $GalT^{-MCP/-MCP}$ pigs were 93.9%, 82.4%, 89.9%, 83.9%, 87.4%, 92.8%, and 83.6%, respectively. The rates of spermatozoa with normal acrosome integrity in WT, Factor 8, vWF, hTPA, hEPO, $GalT^{-MCP/+}/CD73$, and $GalT^{-MCP/-MCP}$ pigs were 98.1%, 98.6%, 98.6%, 98.7%, 98.1%, 99.5%, and 95.1%, respectively. There were no significant differences in motility, morphology, viability, and acrosome integrity of the spermatozoa among WT, Factor 8, vWF, hTPA, and hEPO, $GalT^{-MCP/+}/CD73$, and $GalT^{-MCP/-MCP}$ pigs. These mean that neither random integration nor targeted integration of the transgene into chromosome of pig effect on characteristics of spermatozoa. Ultimately, the transgenic male pigs subjected in this study could apply to propagate their progenies for production of human therapeutic proteins and advancing the xenotransplantation research.

Keywords

References

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