Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effects of Heat Stress on the Developmental Competence of Bovine Cumulus-Oocyte Complex During in vitro Maturation

Heat Stress가 소 난자의 체외성숙과 배반포 발달에 미치는 영향

  • Kim, Min-Su (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Chan-Lan (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Seong, Hwan-Hoo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Namtae (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Sung Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
  • 김민수 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김찬란 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 성환후 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김남태 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김성우 (농촌진흥청 국립축산과학원 가축유전자원센터)
  • Received : 2017.02.10
  • Accepted : 2017.05.17
  • Published : 2017.09.30
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Abstract

The elevated temperature and high humidity has been known as main reason for heat stress on animals and cause detrimental effects on productivity of organisms and physiological conditions of normal bioactivities. The aims of this study were to evaluate the relationship between time of heat shock simulation during in vitro maturation and developmental competence of subsequent embryo after in vitro fertilization. Heat shocked cumulus-oocyte complexes (COCs) of Korean native cattle were subjected to normal conditions for 22, 21, 18 and 12 h respectively and transferred to heat stress inducing condition at $40.5^{\circ}C$ in other incubator for 0 (control), 1 and 4 h. After maturation for 22 h, the oocytes were fertilized and cultured in mSOF media for 8 d and examined the developmental capacity of embryos. There were no differences in maturation and cleavage rates between 0, 1 and 4 h heat socked oocytes, but blastocysts formation were lower in the 4 h heat stressed oocytes. The apoptotic cells of developed blastocysts were also increased in at day 8 with 4 h heat shocked oocytes. These results indicate that heat shock on oocytes during maturation could cause negative effects on the developmental competence of embryos.

혹서기에 있어 고온 다습한 환경은 동물의 생산성과 생리적 반응에 영향을 주어 HS를 유도하는것으로 알려져 있다. 본 연구에서 HS처리 효과가 도축장 유래 난소에서 채취한 난자의 체외 성숙율, 난할율 및 수정란의 발생능력에 미치는 영향을 비교 검토하였다. 대조군으로서 COCs를 $38.5^{\circ}C$에서 22시간 배양하였으며 실험군은 전배양을 동일하게 21, 18 및 12시간 배양 후 $40.5^{\circ}C$에서 각각 1, 4 및 12시간 동안 후배양하여 HS를 유도하였다. 22 시간 숙성시킨 후, COCs를 체외수정하여 mSOF 배지에서 8 일 동안 배양하였을 때 난자의 성숙율과 수정란의 발생 능력을 조사 하였다. 대조군과 1 및 4 시간동안 HS처리된 난자에서 성숙율과 난할율에는 차이가 없었으나(p > 0.05), 4 시간 HS처리군에서 배반포 형성율이 유의적으로 감소하였다(p < 0.05). 또한, 4시간 이상의 지속적인 HS에 대한 노출은 배반포 형성율과 세포사멸도에 영향을 주는 것으로 관찰되었다(p < 0.05). 이러한 결과는 HS가 난자의 성숙 과정에서 유도되면 수정란의 발생 능력에 부정적인 영향을 줄 수 있음을 시사하며 HS에 의한 소 배반포에서 세포사멸현상이 나타나고 있음을 보여주고 있다.

Keywords

References

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