Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enhancement of Lysine Production in Recombinant Corynebacterium glutamicum through Expression of Deinococcus radiodurans pprM and dr1558 Genes

Deinococcus radiodurans 유래 DR1558과 PprM에 의한 Corynebacterium glutamicum의 라이신 생산 향상 연구

  • Kim, Su-mi (Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy & Biomaterials, Chonnam National University) ;
  • Lim, Sangyong (Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Park, Si Jae (Division of Chemical Engineering and Materials Science, Ewha Womans University) ;
  • Joo, Jeong Chan (Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology) ;
  • Choi, Jong-il (Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy & Biomaterials, Chonnam National University)
  • 김수미 (전남대학교생물공학과, 바이오에너지및 바이오소재협동과정) ;
  • 임상용 (한국원자력연구원 첨단방사선연구소) ;
  • 박시재 (이화여자대학교 화학신소재공학과) ;
  • 주정찬 (한국화학연구원 바이오화학센터) ;
  • 최종일 (전남대학교생물공학과, 바이오에너지및 바이오소재협동과정)
  • Received : 2017.08.23
  • Accepted : 2017.08.25
  • Published : 2017.09.28
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Abstract

The expression of Deinococcus radiodurans dr1558 and pprM genes was examined for enhanced lysine production in recombinant Corynebacterium glutamicum. These genes are known to confer high tolerance to pH and osmotic shock in Escherichia coli. D. radiodurans dr1558 and pprM genes were expressed in C. glutamicum by using 6 synthetic promoters of different strengths, to evaluate the effect of expression efficiency on lysine production. Recombinant C. glutamicum expressing DR1558 under the L26 and I64 promoters showed higher lysine production than that expressing DR1558 under other promoters. Similarly, recombinant C. glutamicum expressing PprM under same promoters (L26 and I64) showed a higher increase in lysine production compared to that expressing PprM under other promoters. In the absence of $CaCO_3$ in the medium, the expression of DR1558 or PprM also increased lysine concentration in C. glutamicum depending on the promoter used. Together, these results suggest that genes involved in radiation tolerance in D. radiodurans can be used to enhance production of amino acids and their derivatives.

Keywords

References

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