Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification and Characterization of Agar-degrading Vibrio sp. GNUM08123 Isolated from Marine Red Macroalgae

한천분해 미생물 Vibrio sp. GNUM08123의 동정 및 agarase 생산의 발효적 특성

  • Chi, Won-Jae (Biological and Genetic Resource Assessment Division, National Institute of Biological Resource) ;
  • Kim, Yoon Hee (Department of Biological Science, Myongji University) ;
  • Kim, Jong-Hee (Department of Food and Nutrition, Seoil University) ;
  • Hong, Soon-Kwang (Department of Biological Science, Myongji University)
  • 지원재 (국립생물자원관 유용자원분석과) ;
  • 김윤희 (명지대학교 생명과학정보학부) ;
  • 김종희 (서일대학교 식품영양과) ;
  • 홍순광 (명지대학교 생명과학정보학부)
  • Received : 2017.07.24
  • Accepted : 2017.08.24
  • Published : 2017.09.28
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Abstract

An agar-degrading bacterium, designated as the GNUM08123 strain, was isolated from samples of red algae collected from the Yongil Bay near East Sea, Korea. The isolated GNUM08123 strain was gram-negative, aerobic, motile, and beige-pigmented, with $C_{16:0}$ (25.9%) and summed feature 3 (comprising $C_{16:1}{\omega}7c/iso-C_{15:0}2-OH$, 34.4%) as its major cellular fatty acids. A similarity search based on the 16S rRNA gene sequence revealed that it belonged to class Gammaproteobacteria and shared 97.7% similarity with the type strain Vibrio chagasii $R-3712^T$. The DNA G+C content of strain $GNUM08123^T$ was 46.9 mol%. The major isoprenoid quinone was ubiquinone-8. The results of DNA-DNA relatedness and 16S rRNA sequence similarity analyses, in addition to its phenotypic and chemotaxonomic characteristics, suggest that strain GNUM08123 is a novel species within genus Vibrio, designated as Vibrio sp. GNUM08123. Agarase production by strain GNUM08123 was induced by agar and sucrose, but was repressed probably owing to carbon catabolite repression by glucose and maltose.

Keywords

References

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