International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Role of Recombinant PnTx2-6 Protein as a Mediator of Vasodilation in Blood Vessels

  • Park, Seung-Won (Department of Biotechnology, Daegu Catholic University) ;
  • Kim, Seong Ryul (Sericultural and Apicultural Materials Division, National Institute of Agricultural Science, RDA) ;
  • Goo, Tae-Won (Department of Biochemistry, Dongguk University College of Medicine) ;
  • Choi, Kwang-Ho (Sericultural and Apicultural Materials Division, National Institute of Agricultural Science, RDA)
  • Received : 2017.08.03
  • Accepted : 2017.09.05
  • Published : 2017.09.30
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Abstract

The venome of Phoneutria nigriventer spider has been shown to have side effects including severe painful erections that last for hours. PnTx2-6, a toxin from P. nigriventer spider venom, modulates voltage gated $Na^+$ channels and activation of nitric oxide (NO) production. NO is essential for the regulation of blood flow and pressure. Therefore, PnTx2-6 is expected to be effective not only for erectile dysfunction but also for cardiovascular diseases. A previously has reported cDNA clone for PnTx2-6 toxin, which was expressed in E. coli cytoplasm. We created the same clone and expressed it in a bacterial expression system. PnTx2-6 increased the genes expression of superoxide dismutase 1, glutathione peroxidase 1, and sulfiredoxin 1. We hypothesized that recombinant PnTx2-6 may indirectly regulate blood flow and pressure, resulting in NO production in human umbilical vein endothelial cells (HUVEC). These data suggest differential regulation of the vascular ageing process, which may contribute to the anatomic heterogeneity of atherosclerosis. The results of this study may be used for the emergency treatment of sudden cardiovascular disease caused by ageing.

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References

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