DOI QR코드

DOI QR Code

Inhibitory Effect of Hizikia fusiformis Solvent-Partitioned Fractions on Invasion and MMP Activity of HT1080 Human Fibrosarcoma Cells

  • Lee, Seul-Gi (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University) ;
  • Karadeniz, Fatih (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University) ;
  • Oh, Jung Hwan (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University) ;
  • Yu, Ga Hyun (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University) ;
  • Kong, Chang-Suk (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University)
  • Received : 2017.04.12
  • Accepted : 2017.08.21
  • Published : 2017.09.30

Abstract

Matrix metalloproteinases (MMPs) are endopeptidases that take significant roles in extracellular matrix degradation and therefore linked to several complications such as metastasis of cancer progression, oxidative stress, and hepatic fibrosis. Hizikia fusiformis, a brown algae, was reported to possess bioactivities, including but not limited to, antiviral, antimicrobial, and anti-inflammatory partly due to bioactive polysaccharide contents. In this study, the potential of H. fusiformis against cancer cell invasion was evaluated through the MMP inhibitory effect in HT1080 fibrosarcoma cells in vitro. H. fusiformis crude extract was fractionated with organic solvents, $H_2O$, n-BuOH, 85% aqueous MeOH, and n-hexane (n-Hex). The non-toxicity of the fractions was confirmed by MTT assay. All fractions inhibited the enzymatic activities of MMP-2 and MMP-9 according to the gelatin zymography assay. Cell migration was also significantly inhibited by the n-Hex fraction. In addition, both gene and protein expressions of MMP-2 and -9, and tissue inhibitor of MMPs (TIMPs) were evaluated by reverse transcription-polymerase chain reaction and Western blotting, respectively. The fractions suppressed the mRNA and protein levels of MMP-2, MMP-9 while elevating the TIMP-1 and TIMP-2, with the $H_2O$ fraction being the least effective while n-Hex fraction the most. Collectively, the n-Hex fraction from brown algae H. fusiformis could be a potential inhibitor of MMPs, suggesting the presence of various derivatives of polysaccharides in high amounts.

Keywords

References

  1. Deryugina EI, Quigley JP. 2015. Tumor angiogenesis: MMP-mediated induction of intravasation- and metastasis-sustaining neovasculature. Matrix Biol 44-46: 94-112. https://doi.org/10.1016/j.matbio.2015.04.004
  2. Shuman Moss LA, Jensen-Taubman S, Stetler-Stevenson WG. 2012. Matrix metalloproteinases: changing roles in tumor progression and metastasis. Am J Pathol 181: 1895-1899. https://doi.org/10.1016/j.ajpath.2012.08.044
  3. Shay G, Lynch CC, Fingleton B. 2015. Moving targets: emerging roles for MMPs in cancer progression and metastasis. Matrix Biol 44-46: 200-206. https://doi.org/10.1016/j.matbio.2015.01.019
  4. Gialeli C, Theocharis AD, Karamanos NK. 2011. Roles of matrix metalloproteinases in cancer progression and their pharmacological targeting. FEBS J 278: 16-27. https://doi.org/10.1111/j.1742-4658.2010.07919.x
  5. Thomas NV, Kim SK. 2010. Metalloproteinase inhibitors: status and scope from marine organisms. Biochem Res Int 2010: 845975.
  6. Hsiao WL, Liu L. 2010. The role of traditional Chinese herbal medicines in cancer therapy - from TCM theory to mechanistic insights. Planta Med 76: 1118-1131. https://doi.org/10.1055/s-0030-1250186
  7. Kim Y, Kang H, Jang SW, Ko J. 2011. Celastrol inhibits breast cancer cell invasion via suppression of NF-${\kappa}B$-mediated matrix metalloproteinase-9 expression. Cell Physiol Biochem 28: 175-184. https://doi.org/10.1159/000331729
  8. Wahl O, Oswald M, Tretzel L, Herres E, Arend J, Efferth T. 2011. Inhibition of tumor angiogenesis by antibodies, synthetic small molecules and natural products. Curr Med Chem 18: 3136-3155. https://doi.org/10.2174/092986711796391570
  9. Orlikova B, Diederich M. 2012. Power from the garden: plant compounds as inhibitors of the hallmarks of cancer. Curr Med Chem 19: 2061-2087. https://doi.org/10.2174/092986712800228998
  10. Kuete V, Tankeo SB, Saeed ME, Wiench B, Tane P, Efferth T. 2014. Cytotoxicity and modes of action of five Cameroonian medicinal plants against multi-factorial drug resistance of tumor cells. J Ethnopharmacol 153: 207-219. https://doi.org/10.1016/j.jep.2014.02.025
  11. Wijesekara I, Pangestuti R, Kim SK. 2011. Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae. Carbohydr Polym 84: 14-21. https://doi.org/10.1016/j.carbpol.2010.10.062
  12. Liu M, Hansen PE, Lin X. 2011. Bromophenols in marine algae and their bioactivities. Mar Drugs 9: 1273-1292. https://doi.org/10.3390/md9071273
  13. Khanavi M, Gheidarloo R, Sadati N, Ardekani MRS, Nabavi SMB, Tavajohi S, Ostad SN. 2012. Cytotoxicity of fucosterol containing fraction of marine algae against breast and colon carcinoma cell line. Pharmacogn Mag 8: 60-64. https://doi.org/10.4103/0973-1296.93327
  14. Zhu T, Heo HJ, Row KH. 2010. Optimization of crude polysaccharides extraction from Hizikia fusiformis using response surface methodology. Carbohydr Polym 82: 106-110. https://doi.org/10.1016/j.carbpol.2010.04.029
  15. Choi JI, Kim HJ, Kim JH, Chun BS, Ahn DH, Kim GH, Lee JW. 2010. Changes in colour and antioxidant activities of Hizikia fusiformis cooking drips by gamma irradiation. LWT-Food Sci Technol 43: 1074-1078. https://doi.org/10.1016/j.lwt.2010.02.012
  16. Li YX, Wijesekara I, Li Y, Kim SK. 2011. Phlorotannins as bioactive agents from brown algae. Process Biochem 46: 2219-2224. https://doi.org/10.1016/j.procbio.2011.09.015
  17. Harnedy PA, FitzGerald RJ. 2011. Bioactive proteins, peptides, and amino acids from macroalgae. J Phycol 47: 218-232. https://doi.org/10.1111/j.1529-8817.2011.00969.x
  18. Siriwardhana N, Jeon YJ, Kim SH, Ha JH, Heo SJ, Lee KW. 2004. Enzymatic hydrolysis for effective extraction of antioxidative compounds from Hizikia fusiformis. Algae 19: 59-68. https://doi.org/10.4490/ALGAE.2004.19.1.059
  19. Bae MJ, Karadeniz F, Ahn BN, Kong CS. 2015. Evaluation of effective MMP inhibitors from eight different brown algae in human fibrosarcoma HT1080 cells. Prev Nutr Food Sci 20: 153-161. https://doi.org/10.3746/pnf.2015.20.3.153
  20. Matanjun P, Mohamed S, Mustapha NM, Muhammad K. 2009. Nutrient content of tropical edible seaweeds, Eucheuma cottonii, Caulerpa lentillifera and Sargassum polycystum. J Appl Phycol 21: 75-80. https://doi.org/10.1007/s10811-008-9326-4
  21. Patarra RF, Paiva L, Neto AI, Lima E, Baptista J. 2011. Nutritional value of selected macroalgae. J Appl Phycol 23: 205-208. https://doi.org/10.1007/s10811-010-9556-0
  22. Jiao J, Friedman SL, Aloman C. 2009. Hepatic fibrosis. Curr Opin Gastroenterol 25: 223-239. https://doi.org/10.1097/MOG.0b013e3283279668
  23. Plaza M, Santoyo S, Jaime L, Garcia-Blairsy Reina G, Herrero M, Senorans FJ, Ibanez E. 2010. Screening for bioactive compounds from algae. J Pharm Biomed Anal 51: 450-455. https://doi.org/10.1016/j.jpba.2009.03.016
  24. Phan TT, Hughes MA, Cherry GW. 1998. Enhanced proliferation of fibroblasts and endothelial cells treated with an extract of the leaves of Chromolaena odorata (Eupolin), an herbal remedy for treating wounds. Plast Reconstr Surg 101: 756-765. https://doi.org/10.1097/00006534-199803000-00027
  25. de la Mare JA, Lawson JC, Chiwakata MT, Beukes DR, Edkins AL, Blatch GL. 2012. Quinones and halogenated monoterpenes of algal origin show anti-proliferative effects against breast cancer cells in vitro. Invest New Drugs 30: 2187-2200. https://doi.org/10.1007/s10637-011-9788-0
  26. Egeblad M, Werb Z. 2002. New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer 2: 161-174. https://doi.org/10.1038/nrc745
  27. Li HB, Cheng KW, Wong CC, Fan KW, Chen F, Jiang Y. 2007. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chem 102: 771-776. https://doi.org/10.1016/j.foodchem.2006.06.022
  28. Thippeswamy G, Salimath BP. 2007. Induction of caspase-3 activated DNase mediated apoptosis by hexane fraction of Tinospora cordifolia in EAT cells. Environ Toxicol Pharmacol 23: 212-220. https://doi.org/10.1016/j.etap.2006.10.004
  29. Choi EY, Hwang HJ, Kim IH, Nam TJ. 2009. Protective effects of a polysaccharide from Hizikia fusiformis against ethanol toxicity in rats. Food Chem Toxicol 47: 134-139. https://doi.org/10.1016/j.fct.2008.10.026

Cited by

  1. Antiallergic Effect of Hizikia fusiformis in an Ovalbumin-Induced Allergic Rhinitis Mouse Model vol.12, pp.2, 2019, https://doi.org/10.21053/ceo.2019.00094
  2. Hizikia fusiforme extract enhances dendritic cell maturation in vitro and in vivo vol.84, pp.9, 2017, https://doi.org/10.1080/09168451.2020.1772037
  3. Hizikia fusiformis: Pharmacological and Nutritional Properties vol.10, pp.7, 2017, https://doi.org/10.3390/foods10071660