Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Biosynthesis of polyhydroxyalkanoate by mixed microbial cultures from hydrolysate of waste activated sludge

혼합미생물배양체를 이용한 폐활성슬러지 가용화 산물로부터 polyhydroxyalkanoate 생합성

  • Park, Taejun (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Yoo, Young Jae (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Jung, Dong Hoon (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Lee, Sun Hee (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Rhee, Young Ha (Department of Microbiology and Molecular Biology, Chungnam National University)
  • 박태준 (충남대학교 미생물.분자생명과학과) ;
  • 유영재 (충남대학교 미생물.분자생명과학과) ;
  • 정동훈 (충남대학교 미생물.분자생명과학과) ;
  • 이선희 (충남대학교 미생물.분자생명과학과) ;
  • 이영하 (충남대학교 미생물.분자생명과학과)
  • Received : 2017.08.09
  • Accepted : 2017.08.30
  • Published : 2017.09.30
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Abstract

A new approach to the solubilization of waste activated sludge (WAS) using alginate-quaternary ammonium complex beads was investigated under controlled mild alkaline conditions. The complex beads were prepared by the reaction of sodium alginate (SA) with 3-(trimethoxysilyl)propyl-octadecyldimethylammonium chloride (TSA) in acid solution, followed by crosslinking with $CaCl_2$. Treatment of WAS with SA-TSA complex beads was effective for enhancing the efficacy of WAS solubilization. The highest value of soluble chemical oxygen demand (SCOD) concentration (3,900 mg/L) was achieved after 10 days of treatment with 30% (v/v) SA-TSA complex beads. The WAS solubilization efficacy of the complex beads was also evaluated by estimating the concentrations of volatile fatty acids (VFAs). The maximum value of VFAs was 2,961 mg/L, and the overall proportions of VFAs were more than 75% of SCOD. The main components of VFAs were acetic, propionic, iso-butyric, and butyric acids. These results suggest that SA-TSA complex beads might be useful for enhancing the solubilization of WAS. The potential use of VFAs as the external carbon substrate for the production of polyhydroxyalkanoate (PHA) by a mixed microbial culture (MMC) was also examined. The enrichment of PHA-accumulating MMC could be achieved by periodic feeding of VFAs generated from WAS in a sequencing batch reactor. The composition of PHA synthesized from VFAs mainly consisted of 3-hydroxybutyrate. The maximum PHA content accounted for 25.9% of dry cell weight. PHA production by this process is considered to be promising since it has a doubly beneficial effect on the environment by reducing the amount of WAS and concomitantly producing an eco-friendly biopolymer.

SA-TSA 복합구를 이용해 폐활성슬러지로부터 VFAs를 생산하고, 이를 발효 기질로 이용해 PHA를 생산하기 위한 실험을 수행하였다. 30%의 SA-TSA 복합구를 처리하였을 때 폐활성슬러지의 가용화 효율이 가장 효과적으로 일어나서 약 3,900 mg/L의 SCOD값을 얻었으며, acetic acid, propionic acid, iso-butyric acid 및 butyric acid를 주성분으로 하는 2,961 mg/L 농도의 VFAs를 얻었다. VFAs를 외부탄소원으로 이용하는 SBR 공정을 통해 PHA를 생합성하는 미생물을 농화배양하는 실험을 수행하고 PCR-DGGE로 분석한 결과, 우점 미생물로 Vibrio spp.와 Corynebacterium glutamicum이 확인되었다. 폐활성슬러지로부터 얻은 VFAs를 탄소원으로 사용하여 농화된 혼합미생물배양체를 회분배양한 결과, 건체량의 25.9%에 달하는 PHB를 얻었다. 본 연구결과는 SA-TSA 복합구를 이용하여 폐활성슬러지로부터 VFAs를 얻음으로써 폐슬러지 감량화 효과를 얻고, 또 혼합미생물배양체를 이용하여 VFAs를 바이오폴리머로 전환함으로써 경제성을 확보할 수 있는 새로운 생물공정의 가능성을 보여준다.

Keywords

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