Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Identification of Lactobacillus spp. associated with nematodes in peach farm soil

복숭아 농장 토양에서 Nematodes와 연관된 Lactobacillus spp.의 분리 및 동정

  • Lee, Woo-Hyun (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
  • Choi, Jae Im (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
  • Lee, Jin Il (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
  • Lee, Won-Pyo (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
  • Yoon, Sung-Sik (Division of Biological Science and Technology, College of Science and Technology, Yonsei University)
  • 이우현 (연세대학교 생명과학기술학부) ;
  • 최재임 (연세대학교 생명과학기술학부) ;
  • 이진일 (연세대학교 생명과학기술학부) ;
  • 이원표 (연세대학교 생명과학기술학부) ;
  • 윤성식 (연세대학교 생명과학기술학부)
  • Received : 2017.05.16
  • Accepted : 2017.09.04
  • Published : 2017.09.30
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Abstract

Strains D4 and D5 were isolated from peach-rotten soil during the peach harvest season. The isolates were identified based on morphological and biochemical characterization, and identification was determined by 16S rRNA gene sequencing. Results showed that D4 has high similarity to Lactobacillus plantarum ATCC $14917^T$ and Lactobacillus pentosus ATCC $8041^T$ at 99.05% and 98.98%, respectively. D5 was also similar to Lactobacillus pentosus ATCC $8041^T$ and Lactobacillus plantarum ATCC $14917^T$ at 98.71% and 98.64%, respectively. In contrast, isolates showed differences in carbohydrate utilization in comparison to Lactobacillus plantarum ATCC $14917^T$ and Lactobacillus pentosus ATCC $8041^T$. In view of this we performed VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis, multiplex PCR fingerprinting, and random amplified polymorphic DNA (RAPD)-PCR to further confirm the identification of D4 and D5. The results of these analyses showed that both strains were most similar to Lactobacillus plantarum.

복숭아 수확시기에 낙과한 토양에서 Lactobacillus sp. D4와 D5 균주를 분리하였다. 분리한 Lactobacillus sp. D4와 D5 균주를 동정하기 위하여 형태학적 동정, 생화학적 동정 및 16S rRNA 유전자서열 분석을 수행하였다. 16S rRNA 유전자서열 분석 결과 Lactobacillus sp. D4는 Lactobacillus plantarum subsp. plantarum ATCC $14917^T$과 Lactobacillus pentosus ATCC $40997^T$에 각각 99.05%, 98.98% 일치하였으며, Lactobacillus sp. D5는 Lactobacillus pentosus ATCC $40997^T$, Lactobacillus plantarum subsp. plantarum ATCC $14917^T$에 각각 98.71%, 98.64% 일치하였다. Lactobacillus sp. D4와 D5 균주는 당 이용성 비교에서 Lactobacillus plantarum ATCC $14917^T$과 Lactobacillus pentosus ATCC $8041^T$에 비교하여 다른 결과를 나타내었다. 정확한 동정을 위하여 VITEK MS matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) 분석, multiplex PCR, random amplified polymorphic DNA (RAPD)-PCR을 수행하였다. 이러한 결과에 근거하여 Lactobacillus sp. D4와 D5 균주는 Lactobacillus plantarum으로 동정되었다.

Keywords

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