The Korean Journal of Oral and Maxillofacial Pathology (대한구강악안면병리학회지)

Korean Academy of Oral and Maxillofacial Pathology (대한구강악안면병리학회)
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Lycorine induces apoptosis by enhancing protein degradation of survivin in human oral cancer cell lines

Lycorine의 사람 구강 암 세포주에서 survivin 단백질 분해 증진으로 세포자멸사 유도

  • Jeong, Joseph H. (Department of Developmental Biology and Genomics, College of Veterinary Medicine, Seoul National University and Korea Mouse Phenotyping Center) ;
  • Cho, Nam-Pyo (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience and Biodegradable material, Chonbuk National University) ;
  • Jang, Boonsil (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience and Biodegradable material, Chonbuk National University)
  • 정요셉 (서울대학교 수의과대학 발육 생유전학과 및 한국생쥐표현형센터) ;
  • 조남표 (전북대학교 치의학대학원 구강병리학교실 및 구강 생명과학 생분해물질 연구소) ;
  • 장분실 (전북대학교 치의학대학원 구강병리학교실 및 구강 생명과학 생분해물질 연구소)
  • Received : 2016.11.28
  • Accepted : 2017.01.31
  • Published : 2017.02.28
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Abstract

Lycorine, a natural alkaloid extracted from the Amaryllidaceae plant family, was reported to various physiological and pharmacological effects including anti-cancer activity. Nevertheless, there is no report of the anticancer effect of lycorine in oral cancer cells. The effects of lycorine on cell proliferation and apoptosis were examined through trypan blue exclusion assay, 4'-6-diamidino-2-phenylindole (DAPI) stain, Live/Dead assay, Western blot analysis and RT-PCR. Lycorine suppressed cell viability and induced apoptosis in MC3 and HSC-3 cell lines. Lycorine decreased survivin protein but did not affect its mRNA. It regulated survivin through accelerating protein degradation in a time-dependent manner although neither proteasome nor lysosome was not associated with lycorine-mediated protein degradation. Collectively, our results suggest that lycorine may be a potential therapeutic anti-cancer drug candidate for the treatment of human oral cancer.

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