Journal of Applied Biological Chemistry

  • 제60권3호
  • /
  • Pages.283-291
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  • 2017
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  • 1976-0442(pISSN)
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  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
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α-amylase를 이용하여 제조한 쌀 페이스트의 품종에 따른 휘발성비휘발성 향미성분 비교분석

Comparative analysis of volatile and non-volatile flavor compounds in rice paste made by α-amylase according to cultivars

  • Son, Eun Young (Department of Food Science and Engineering, Ewha Womans University) ;
  • Kim, Hye Won (Department of Food Science and Engineering, Ewha Womans University) ;
  • Kim, Sun Ah (Department of Food Science and Engineering, Ewha Womans University) ;
  • Lee, Sang Mi (Department of Food Science and Engineering, Ewha Womans University) ;
  • Paek, Se Hee (Food R&D center, CJ Cheiljedang) ;
  • Kim, Sun Hee (Food R&D center, CJ Cheiljedang) ;
  • Seo, Yong Ki (Food R&D center, CJ Cheiljedang) ;
  • Park, Hye-Young (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Oh, Sea-Kwan (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Young-Suk (Department of Food Science and Engineering, Ewha Womans University)
  • 투고 : 2017.07.31
  • 심사 : 2017.09.06
  • 발행 : 2017.09.29
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초록

쌀은 전세계 인구의 절반이 주식으로 사용하고 있으며, 대부분 아시아에서 생산되고 소비되고 있다. 그러나 최근 식생활이 다양해지면서 쌀 소비량은 감소하고 있는 추세이다. 이에 쌀의 활용도를 높이기 위한 다양한 시도가 요구되고 있다. 본 연구에서는 ${\alpha}$-amylase로 처리된 9종의 서로 다른 품종의 쌀 페이스트를 고체상 미세추출법과 가스 크로마토그래피-질량분석법을 이용하여 휘발성 성분들을 프로파일링하고, 아미노산, 당류 및 당알콜 등의 비휘발성 향미성분들은 유도체화 처리 후 가스 크로마토그래피-시간 비행형-질량분석법을 이용하여 비교분석을 수행하였다. 총 46종의 휘발성 성분들이 검출되었으며, 이에는 6종의 알콜류, 6 종의 알데히드류, 4종의 에스터류, 4종의 퓨란류, 4종의 케톤류, 1 종의 산, 1종의 황 함유 성분, 7종의 탄화수소류, 5종의 벤젠류 및 8종의 터핀류 등을 포함되어 있다. 비휘발성 향미성분들에는 12종의 아미노산, 6종의 당, 4종의 당알콜이 동정되었다. 휘발성 및 비휘발성 향미성분 분석에 근거한 주성분분석에 의해 서로 다른 쌀 페이스트 시료들을 구분할 수 있었다. 휘발성 성분의 경우 pentanal과 4,7-dimethylundecane는 서농 17호 백미와 서농 17호 현미를 구분 짓는 성분이었으며, 이에 비해 일품은 다른 품종들과 달리 ethanol, 6-methylhep-5-en-2-one, tridecane과 같은 성분에서 차이를 보였다. 비휘발성 성분의 경우 glycine, serine, ${\gamma}$-aminobutyric acid 같은 아미노산들과 sucrose, fructose 같은 당류가 다른 품종들과 단미를 구분 짓는데 기여하는 성분이었다. 한편, galactose, arabitol, mannose는 서농 17호 현미에 비해 서농 17호 백미와 관련이 높은 성분들이었다.

Rice that the half of population in the world eats as a staple food is mostly produced and consumed in Asia. However, its consumption is nowadays decreasing mainly due to diet diversity. Accordingly, some attempts are in demand to enhance the utilization of rice. In this study, profiling of volatile and non-volatile flavor components in rice pastes obtained by ${\alpha}$-amylase was performed and compared according to nine different rice cultivars domestically cultivated in Korea using gas chromatography-mass spectrometry combined by solid phase microextraction and gas chromatography-time of flight-mass spectrometry after a derivatization, respectively. In total, 46 volatile compounds identified included 6 alcohols, 6 aldehydes, 4 esters, 4 furan derivatives, 4 ketones, 1 acid, 1 sulfur-containing compound, 7 hydrocarbons, 5 aromatics and 8 terpenes. The non-volatile flavor components found were composed of 12 amino acids, 6 sugars and 4 sugar alcohols. In principal component analysis, rice paste samples could be discriminated according to cultivars on the score plots of volatile and non-volatile flavor compounds. In particular, some volatile compounds such as pentanal and 4,7-dimethylundecane could contribute to distinguish Senong 17 white and Senong 17 brown, whereas ethanol, 6-methylhep-5-en-2-one, and tridecane could be highly related to the discrimination of Iipum from other cultivars. Among non-volatile compounds, some amino acids such as glycine, serine and ${\gamma}$-aminobutyric acid and some sugars such as sucrose and fructose were mainly responsible for the discrimination of Danmi from the other cultivars. On the other hand, galactose, arabitol and mannose were more closely related to Senong 17 white than Senong 17 brown.

키워드

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