Analysis and Stability Test of the Extracts from Astragali Radix, Paeoniae Radix, and Corni Fructus for Toxicity Study

황기, 작약 및 산수유의 독성평가를 위한 성분분석 및 안정성 시험

  • Received : 2017.08.22
  • Accepted : 2017.09.15
  • Published : 2017.09.30

Abstract

A simple and reliable reverse phase HPLC method was developed to determine pharmacologically active marker compounds of Astragali Radix, Paeoniae Radix, and Corni Fructus. The stability test of water-extract from three natural medicines were examined for six months. However, no significant changes in the content of the marker compounds of each extract were observed during the investigation.

Keywords

References

  1. Kim, S. H., Choi, E. J., Kim, D. H., Lee, K. Y., Lee, M., Baek, S. W., Kwak, S. J., Kang, T. S., Kim, Y. C. and Sung, S. H. (2008) Stability test of the extracts of Cimicifugae Rhizoma, Achyranthis Radix, Artemisia Capillaris Herba, Moutan Cortex Radicis and Arecae Semen for toxicity study. Kor. J. Pharmacogn. 39: 241-245.
  2. Park, C. H. and Kwack, S. J. (2009) The effects of aristolochic acid on reproductive function in female rats. Kor. J. Pharmacogn. 40: 89-98.
  3. Frei, H., Wurgler, F. E., Juon, H., Hall, C. B. and Graf, U. (1985) Aristolochic acid is mutagenic and recombinogenic in Drosophila genotoxicity tests. Arch. Toxicol. 56: 158-166. https://doi.org/10.1007/BF00333420
  4. Mengs, U. (1987) Acute toxicity of aristolochic acid in rodents. Arch. Toxicol. 59: 328-331. https://doi.org/10.1007/BF00295084
  5. Arlt, V. M., Annie, P. L., Cosyns, J. P. and Schmeiser, H. H. (2001) Analyses of DNA adducts formed by ochratoxin A and aristolochic acid in patients with Chinese herbs nephropathy. Mutat. Res. 494: 143-150. https://doi.org/10.1016/S1383-5718(01)00188-7
  6. Song, J. Z., Yiu, H. H., Qiao, C. F., Han, Q. B. and Xu, H. X. (2008) Chemical comparison and classification of Radix Astragali by determination of isoflavonoids and astragalosides. J. Pharm. Biomed. Anal. 47: 399-406. https://doi.org/10.1016/j.jpba.2007.12.036
  7. Motomura, K., Fujiwara, Y., Kiyota, N., Tsurushima, K., Takeya, M., Nohara, T., Nagai, R. and Ikeda, T. (2009) Astragalosides isolated from the root of Astragalus Radix inhibit the formation of advanced glycation and products. J. Agric. Food Chem. 57: 7666-7672. https://doi.org/10.1021/jf9007168
  8. Fu, J., Wang, Z., Huang, L., Zheng, S., Wang, D., Chen, S., Zhang, H. and Yang, S. (2014) Review of the botanical charateristics, phytochemistry, and pharmacology of Astragalus membranaceus (Huanggi). Phytother. Res. 28: 1275-1283. https://doi.org/10.1002/ptr.5188
  9. Zhou, X., Sun, X., Gong, X., Yang, Y., Chen, C., Shan, G. and Yao, Q. (2017) Astragaloside IV from Astragalus mambranaceus ameliorates renal intestitial fibrosis by inhibiting inflammation via TLR4/NF-${\kappa}B$ in vivo and in vitro. Int. Immunopharmacol. 42: 18-24. https://doi.org/10.1016/j.intimp.2016.11.006
  10. Lv, J., Zhang, Y., Tian, Z., Liu, F., Shi, Y., Liu, Y. and Xia, P. (2017) Astragalus polysaccharides protect against dextran sulfate sodium-induces colitis by inhibiting NF-${\kappa}B$ activation. Int. J. Biol. Macromol. 98: 723-729. https://doi.org/10.1016/j.ijbiomac.2017.02.024
  11. Li, V. V., Sun, Y. N., Yan, X. T., Yang, S. Y., Kim, S., Lee, Y. M., Koh, Y. S. and Kim, Y. H. (2014) Flavonoids from Astragalus membranaceus and their inhibitory effects on LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendritic cells. Arch. Pharm. Res. 37: 186-192. https://doi.org/10.1007/s12272-013-0174-7
  12. Wang, H. B., Gu, W. F., Chu, W. J., Zhang, S., Tang, X. C. and Qin, G. W. (2009) Monoterpene glycoside from Paeonia lactiflora. J. Nat. Prod. 72: 1321-1324. https://doi.org/10.1021/np8001783
  13. Tanaka, T., Fukumori, M., Ochi, T. and Kouno, I. (2003) Paeonianins A-E, new dimeric and monomeric ellagitannins from the fruits of Paeonia lactiflora. (2003) J. Nat. Prod. 66: 759-763. https://doi.org/10.1021/np020608g
  14. Shi, Y. H., Zhu, S., Ge, Y. W., He, Y. N., Kazuma, K., Wang, Z., Yoshimatsu, K. and Komatsu, K. (2016) Monoterpene derivatives with anti-allergic activity from red peony root, the root of Paeonia lactiflora. Fitoterapia 108: 55-61. https://doi.org/10.1016/j.fitote.2015.11.011
  15. Zhang, L., Yang, B. and Yu, B. (2015) Paeoniflorin protects against nonalcoholic fatty liver disease induced by a high-fat diet in mice. Bio. Pharm. Bull. 38: 1005-1011. https://doi.org/10.1248/bpb.b14-00892
  16. Liu, X., Yang, M. H., Wang, X. B., Xie, S. S., Li, Z. R., Kim, D. H., Park, J. S. and Kong, L. Y. (2015) Lignans from the root of Paeonia lactiflora and their anti-${\beta}$-amyloid aggregation activities. Fitoterapia 103: 136-142. https://doi.org/10.1016/j.fitote.2015.03.011
  17. Tsai, H. Y., Lin, H. Y., Fong, Y. C., Wu, J. B., Chen, Y. F., Tsuzuki, M. and Tang, C. H. (2008) Paeonol inhibits RANKL-induced osteoclastogenesis by inhibiting ERK, p38 and NF-kappaB pathway. Eur. J. Pharmacol. 24: 124-133.
  18. Kim, S. J., Jin, M., Lee, E., Moon, T. C., Quan, Z., Yang, J. H., Son, K. H., Kim, K. U., Son, J. K. and Chang, H. W. (2006) Effects of methyl gallate on arachidonic acid metabolizing enzymes: cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells. Arch. Pharm. Res. 29: 874-878. https://doi.org/10.1007/BF02973908
  19. Baumgartner, R. R., Steinmann, D., Heiss, E. H., Atanasov, A. G., Ganzera, M., Stuppner, H. and Dirsch, V. M. (2010) Bioactivity-guided isolation of 1,2,3,4,6-penta-O-galloyl-D-glucopyranose from Paeonia lactiflora rots as a PTP1B inhibitor. J. Nat. Prod. 73: 1578-1581. https://doi.org/10.1021/np100258e
  20. Park, J. Y., Han, A. R., Kil, Y. S., Kang, U., Kim, S. H., Nam, S. J. and Seo, E. K. (2016) A new secoiridoid glycoside from the fruits of Cornus officinalis (Cornaceae). Nat. Prod. Res. 30: 1504-1510. https://doi.org/10.1080/14786419.2015.1115996
  21. Li, Y. C., Yang, J., Wu, X. G., Xu, X. J. and Fu, Q. Y. (2015) Three new iridoids from leaves of Cornus officinalis. J. Asian Nat. Prod. Res. 17: 788-792. https://doi.org/10.1080/10286020.2015.1026809
  22. Jeong, E. J., Kim, T. B., Yang, H., Kang, S. Y., Kim, S. Y., Sung, S. H. and Kim, Y. C. (2012) Neuroprotective iridoid glycosides from Cornus officinalis fruits against glutamate-induced toxicity in HT22 hippocampal cells. Phytomedicine 19: 317-321. https://doi.org/10.1016/j.phymed.2011.08.068
  23. Jiang, W. L., Zhang, S. P., Hou, J. and Zhu, H. B. (2012) Effect of loganin on experimental diabetic nephropathy. Phytomedicine 19: 217-222. https://doi.org/10.1016/j.phymed.2011.08.064
  24. Choi, Y. H., Jin, G. Y., Li, G. Z. and Yan, G. H. (2011) Cornuside suppresses lipopolysaccharide-induced inflammatory mediators by inhibiting nuclear factor-kappa B activation in RAW 264.7 macrophages. Biol. Pharm. Bull. 34: 959-966. https://doi.org/10.1248/bpb.34.959
  25. 식품의약품안전처 고시 2003-17. 안전성유효성심사규정.
  26. Kim, G. S., Lee, D. Y., Lee, S. E., Noh, H. J., Choi, J. H., Park C. G., Choi, S. I., Hong, S. J. and Kim, S. Y. (2013) Evaluation on extraction conditions and HPLC analysis method for bioactive compounds of Astrgali Radix. Koean J. Medicinal Crop Sci. 21: 486-492. https://doi.org/10.7783/KJMCS.2013.21.6.486
  27. 생약종합정보시스템 (http://www.kfda.go.kr/herbmed).