The Korean journal of internal medicine

The Korean Association of Internal Medicine (대한내과학회)
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Caveolin-1 is involved in high glucose accelerated human glomerular mesangial cell senescence

  • Feng, Xin (Department of Rheumatology, The First Affiliated Hospital of Liaoning Medical University) ;
  • Gao, Wei (Department of Rheumatology, The First Affiliated Hospital of Liaoning Medical University) ;
  • Li, Yao (Department of Physiology, Liaoning Medical University)
  • Received : 2015.08.01
  • Accepted : 2015.10.15
  • Published : 2017.09.01
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Abstract

Background/Aims: We demonstrated the role of caveolin-1 involved in high glucose (HG)-induced glomerular mesangial cells (GMCs) senescence. Methods: HG was used to stimulate GMCs. The telomere lengths were analyzed by Southern blot. ${\beta}$-Galactosidase staining was determined. The expressions of caveolin-1 and P53 proteins were determined by Western blot. Results: Treatment with high concentrations of glucose induced GMC senescence accompanied by shortened telomere length and increase of ${\beta}$-galactosidase staining as well as P53 protein, which was abrogated after application of caveolin-1-siRNA. Conclusions: This study proved that HG induced cell senescence in GMCs. The caveolin-1 is involved in HG-induced mesangial cell senescence, and blocking caveolin-1 significantly reduced cell senescence. The effect of caveolin-1 is mediated by P53 pathway.

Keywords

Acknowledgement

Supported by : Liaoning Medical University

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