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Formulation of Wax Type Dispenser Monitoring the Oriental Fruit Fly, Bactrocera dorsalis, and Its Molecular Diagnostic Technology

오리엔탈과실파리 예찰용 왁스형방출기 제작 및 분자진단기술

  • Kim, Yonggyun (Department of Plant Medicals, Andong National University) ;
  • Kim, Minhyun (Department of Plant Medicals, Andong National University) ;
  • Kim, Kyusoon (Department of Plant Medicals, Andong National University) ;
  • Vatanparast, Mohammad (Department of Plant Protection, College of Agriculture, University of Bu-Ali Sina) ;
  • Kim, Yejin (Department of Special Education, Ewha Womans University) ;
  • Kwon, Gimyeon (Bio Utilization Institute)
  • Received : 2017.02.02
  • Accepted : 2017.08.12
  • Published : 2017.09.01

Abstract

The Oriental fruit fly, Bactrocera dorsalis, has been designated as a main quarantine insect pest. Sensitive monitoring and diagnosis against B. dorsalis are required for early quarantine treatment. This study formulated a wax type dispenser containing methyl eugenol and biopesticide to attract and kill this insect. It also developed diagnostic PCR primers against five major quarantine fruit flies of B. dorsalis, B. cucurbitae, B. tryoni, B. latifrons, and Ceratitis capitata. The lure and diagnostic primers were evaluated in a field located in Kota Kinabalu, Malaysia.

오리엔탈과실파리(Bactrocera dorsalis)는 주요 검역 대상으로 지정된 해충이다. 이 해충에 대한 고감도 모니터링과 진단 기술이 초기 방역 처리에 요구된다. 본 연구는 오리엔탈과실파리를 유인하여 치사시킬 목적으로 메틸유제놀과 생물농약을 혼합한 왁스형 방출기를 제작하였다. 본 연구는 또한 5종의 주요 검역 과실파리(오리엔탈과실파리, 오이과실파리, 퀸즐랜드과실파리, 말레이시아과실파리, 지중해과실파리)에 대한 PCR 진단프라이머를 개발하였다. 이상의 모니터링용 왁스방출기과 분자진단기술을 말레이시아 코타키나발루 지역에서 실증 시험하였다.

Keywords

References

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