Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지
DOI QR코드

DOI QR Code

Morphological and Immunological Characterization of Hemocytes in Larvae of Pentodon quadridens bidentulus (Famaire, 1887)

둥글장수풍뎅이 혈구 종류와 식균작용

  • Hwang, Dooseon (Department of Applied Biology, Division of Bioresource Sciences, College of Agriculture and Life Science, Environment Friendly Agriculture Center, Kangwon National University) ;
  • Jang, Young-Chul (Department of Applied Biology, Division of Bioresource Sciences, College of Agriculture and Life Science, Environment Friendly Agriculture Center, Kangwon National University) ;
  • Cho, Saeyoull (Department of Applied Biology, Division of Bioresource Sciences, College of Agriculture and Life Science, Environment Friendly Agriculture Center, Kangwon National University)
  • 황두선 (강원대학교 생물자원과학부 응용생물전공) ;
  • 장영철 (강원대학교 생물자원과학부 응용생물전공) ;
  • 조세열 (강원대학교 생물자원과학부 응용생물전공)
  • Received : 2017.04.07
  • Accepted : 2017.08.01
  • Published : 2017.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to analyze the characteristics of hemocytes in the hemolymph of the larvae of Pentodon quadridens bidentulus and the characteristics of the hemocytes responsible for cellular immunity during pathogen infection. Granulocytes, plasmatocytes, oenocytoids, spherulocytes, prohemocytes and adipohemocytes were found in the circulating hemocytes. Among them, granulocyte were observed as cells responsible for immunological phagocytosis during entry of foreign substances. In particular, it was observed that the most active phagocytic action occurred within 12 hours in vivo, and that after 24 hours, the immune activation was reduced and converted to a normal state. Plasmatocytes were occasionally observed as immunological response, but the remaining hemocytes were not related to immunological activation.

본 연구는 둥글장수풍뎅이(Pentodon quadridens bidentulus (Famaire, 1887)) 유충의 혈림프에 존재하는 혈구세포들의 종류와 병원균 침입시 세포 면역을 담당하는 세포를 특정하고 분석하였다. 둥글장수풍뎅이(P. quadridens bidentulus)유충 혈강내에는 과립혈구세포, 부정형혈구세포, 편도혈구세포, 소구형혈구세포, 원시혈구세포, 지방혈구세포 총 여섯 종류의 혈구세포들이 존재하였다. 여섯 종류의 혈구세포 중 과립혈구세포가 병원균 침입 시 형태적 변이와 함께 병원균들에 대한 식균작용을 수행하는 면역세포로 관찰 되었다. 특히, 생체 내에서 병원균 침입시 12시간 안에 활발히 식균작용을 수행하였고 24시간 이후로는 면역 활성화가 줄어들면서 정상상태로 전환되는 것을 알 수 있었다. 세포질 혈구세포의 식균작용도 간혹 관찰 되었으나 나머지 세포들은 면역과 연관된 활성화가 없는 것으로 나타났다.

Keywords

References

  1. Arnold, J.W., 1974. The hemocytes of insects. Academic Press. New York, U.S.A. pp. 201-254.
  2. Bang, K., Hwang, S., Lee, J., Cho, S., 2015. Identification of immunity-related genes in the larvae of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) by a next-generation sequencing-based transcriptome analysis. J. Insect Sci. 15, 142. https://doi.org/10.1093/jisesa/iev120
  3. Boe, D.M., Curtis, B.J., Chen, M.M., Ippolito, J.A., Kovacs, E.J., 2015. Extracellular traps and macrophages: new roles for the versatile phagocyte. J. Leukoc. Biol. 97, 1023-1035. https://doi.org/10.1189/jlb.4RI1014-521R
  4. Cho, S., 2016. Ultrastructure characterization of hemocytes in larva of Protaetia brevitarsis seulensis. Korean J. Appl. Entomol. 55, 215-221.
  5. Dubovskiy, I.M., Kryukova, N.A., Glupov, V.V., Ratcliffe, N.A., 2016. Encapsulation and nodulation in insects. Invertebr. Survival J. 13, 229-246.
  6. Giglio, A., Battistella, S., Talarico, F.F., Brandmayr, T.Z., Giulianini, P.G., 2008. Circulating hemocytes from larvae and adults of Carabus (Chaetocarabus) lefebvrei Dejean 1826 (Coleoptera, Carabidae): Cell types and their role in phagocytosis after in vivo artificial non-self-challenge. Micron. 39, 552-558. https://doi.org/10.1016/j.micron.2007.07.004
  7. Gupta, A.P., 1979. Insect hemocytes development, forms, functions and techniques. Cambridge University Press. New York, U.S.A. 83 pp.
  8. Hoffmann, J.A., 2003. The immune response of Drosophila. Nature 426, 33-38. https://doi.org/10.1038/nature02021
  9. Hwang, S., Bang, K., Lee, J., Cho, S., 2015. Circulating hemocytes from larvae of the Japanese rhinoceros beetle Allomyrina dichotoma (Linnaeus) (Coleoptera: Scarabaeidae) and the cellular immune response to microorganisms. PLoS ONE 10, e0128519. https://doi.org/10.1371/journal.pone.0128519
  10. Janeway, C.A., Medzhitov, R., 2002. Innate immune recognition. Annu. Rev. Immunol. 20, 197-216. https://doi.org/10.1146/annurev.immunol.20.083001.084359
  11. Jones, J.C., 1962. Current concepts concerning insect hemocytes. Rev. Am. Zool. 2, 209-246. https://doi.org/10.1093/icb/2.2.209
  12. Kaplan, M., Radic, M., 2012. Neutrophil extracellular traps: doubleedged swords of innate immunity. J. Immunol. 189, 2689-2695. https://doi.org/10.4049/jimmunol.1201719
  13. Kim, J., 2011. Arthopoda: Insecta: Coleoptera: Scarabaeoidea: Pleurosticti. Insect Fauna of Korea. NIBR. 12, 120-121.
  14. Kroemer, G., Levine, B., 2008. Autophagic cell death: the story of a misnomer. Nat. Rev. Mol. Cell. Biol. 9, 1004-1010. https://doi.org/10.1038/nrm2529
  15. Krzemien, J., Dubois, L., Makki, R., Meister, M., Vincent, A., Crozatier, M., 2007. Control of blood cell homeostasis in Drosophila larvae by the posterior signalling centre. Nature 446, 325-328. https://doi.org/10.1038/nature05650
  16. Kwon, H., Bang, K., Cho, S., 2014. Characterization of the hemocytes in larvae of Protaetia brevitarsis seulensis: involvement of granulocytemediated phagocytosis. PLoS ONE 9(8), e103620. https://doi.org/10.1371/journal.pone.0103620
  17. Kwon, H., Bang, K., Lee, M., Cho, S., 2014. Molecular cloning and characterization of a lysozyme cDNA from the mole cricket Gryllotalpa Orientalis (Orthoptera: Gryllotalpidae). Mol. Biol. Rep. 41, 5745-5754. https://doi.org/10.1007/s11033-014-3446-5
  18. Lee, J., Bang, K., Hwang, S., Cho, S., 2016. cDNA cloning and molecular characterization of a defensin-like antimicrobial peptide from larvae of Protaetia brevitarsis seulensis (Kolbe). Mol. Biol. Rep. 43, 371. https://doi.org/10.1007/s11033-016-3967-1
  19. Lee, J., Hwang, S., Cho, S., 2016. Immune tolerance to an intestineadapted bacteria, Chryseobacterium sp., injected into the hemocoel of Protaetia brevitarsis seulensis. Sci. Rep. 6, 31722. https://doi.org/10.1038/srep31722
  20. Marino, G., Niso-Santano, M., Baehrecke, E.H., Kroemer, G., 2014. Self-consumption: the interplay of autophagy and apoptosis. Nat. Rev. Mol. Cell. Biol. 15, 81-94. https://doi.org/10.1038/nrm3735
  21. Pandey, J.P., Tiwari, R.K., 2012. An overview of insect hemocyte science and its future application in applied and biomedical fields. Am. J. Biochem. Mole. Biol. 2, 82-105. https://doi.org/10.3923/ajbmb.2012.82.105
  22. Tettamanti, G., Cao, Y., Feng, Q., Grimaldi, A., Eguileor, M., 2011. Autophagy in Lepidoptera: more than old wine in new bottle. 8, 5-14.