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Morphological and Immunological Characterization of Hemocytes in Larvae of Pentodon quadridens bidentulus (Famaire, 1887)

둥글장수풍뎅이 혈구 종류와 식균작용

  • Hwang, Dooseon (Department of Applied Biology, Division of Bioresource Sciences, College of Agriculture and Life Science, Environment Friendly Agriculture Center, Kangwon National University) ;
  • Jang, Young-Chul (Department of Applied Biology, Division of Bioresource Sciences, College of Agriculture and Life Science, Environment Friendly Agriculture Center, Kangwon National University) ;
  • Cho, Saeyoull (Department of Applied Biology, Division of Bioresource Sciences, College of Agriculture and Life Science, Environment Friendly Agriculture Center, Kangwon National University)
  • 황두선 (강원대학교 생물자원과학부 응용생물전공) ;
  • 장영철 (강원대학교 생물자원과학부 응용생물전공) ;
  • 조세열 (강원대학교 생물자원과학부 응용생물전공)
  • Received : 2017.04.07
  • Accepted : 2017.08.01
  • Published : 2017.09.01

Abstract

The purpose of this study was to analyze the characteristics of hemocytes in the hemolymph of the larvae of Pentodon quadridens bidentulus and the characteristics of the hemocytes responsible for cellular immunity during pathogen infection. Granulocytes, plasmatocytes, oenocytoids, spherulocytes, prohemocytes and adipohemocytes were found in the circulating hemocytes. Among them, granulocyte were observed as cells responsible for immunological phagocytosis during entry of foreign substances. In particular, it was observed that the most active phagocytic action occurred within 12 hours in vivo, and that after 24 hours, the immune activation was reduced and converted to a normal state. Plasmatocytes were occasionally observed as immunological response, but the remaining hemocytes were not related to immunological activation.

본 연구는 둥글장수풍뎅이(Pentodon quadridens bidentulus (Famaire, 1887)) 유충의 혈림프에 존재하는 혈구세포들의 종류와 병원균 침입시 세포 면역을 담당하는 세포를 특정하고 분석하였다. 둥글장수풍뎅이(P. quadridens bidentulus)유충 혈강내에는 과립혈구세포, 부정형혈구세포, 편도혈구세포, 소구형혈구세포, 원시혈구세포, 지방혈구세포 총 여섯 종류의 혈구세포들이 존재하였다. 여섯 종류의 혈구세포 중 과립혈구세포가 병원균 침입 시 형태적 변이와 함께 병원균들에 대한 식균작용을 수행하는 면역세포로 관찰 되었다. 특히, 생체 내에서 병원균 침입시 12시간 안에 활발히 식균작용을 수행하였고 24시간 이후로는 면역 활성화가 줄어들면서 정상상태로 전환되는 것을 알 수 있었다. 세포질 혈구세포의 식균작용도 간혹 관찰 되었으나 나머지 세포들은 면역과 연관된 활성화가 없는 것으로 나타났다.

Keywords

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