Korean Journal of Dental Materials (대한치과재료학회지)

Korean Society For Dental Materials (대한치과재료학회)
권호 표지
DOI QR코드

DOI QR Code

Characteristics of Antibacterial Chlorhexidine-Containing Hydroxyapatite Coated on Titanium

타이타늄 상에 코팅된 클로르헥시딘 항균제를 함유한 수산화인회석의 특성

  • Kim, Min-Hee (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University) ;
  • Hwang, Moon-Jin (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University) ;
  • Lee, Woon-Young (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University) ;
  • Park, Yeong-Joon (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University) ;
  • Song, Ho-Jun (Dept. of Dental Materials and Dental Materials Research Institute, School of Dentistry, Chonnam National University)
  • 김민희 (전남대학교 치의학전문대학원 치과재료학교실 및 치과재료연구소) ;
  • 황문진 (전남대학교 치의학전문대학원 치과재료학교실 및 치과재료연구소) ;
  • 이운영 (전남대학교 치의학전문대학원 치과재료학교실 및 치과재료연구소) ;
  • 박영준 (전남대학교 치의학전문대학원 치과재료학교실 및 치과재료연구소) ;
  • 송호준 (전남대학교 치의학전문대학원 치과재료학교실 및 치과재료연구소)
  • Received : 2017.09.05
  • Accepted : 2017.09.16
  • Published : 2017.10.30
⟨ Previous Next ⟩

Abstract

In this study, antibacterial chlorhexidine (CHX)-containing hydroxyapatite (HAp) was coated on titanium and investigated its characteristics. Ti-mSBF-CHX group was prepared by soaking titanium disks in the modified simulated body fluid (mSBF) mixed with CHX. Ti-mSBF group was coated using mSBF without CHX. Ti-mSBF-adCHX group was prepared by soaking Ti-mSBF specimen in CHX-containing solution. The crystallines clusters composed with nano-shaped crystallites were coated on the surface of the Ti-mSBF specimen. The ribbon-shaped crystallites were observed with the crystalline clusters on the Ti-mSBF-CHX specimen. The content of CHX chemical compositions was high in ribbon-shaped crystallites. HAp crystalline structure was dominant for all prepared specimens, and ${\beta}-TCP$ (tricalcium phosphate) and OCP (octacalcium phosphate) crystalline structures were observed in the Ti-mSBF-CHX specimen. FT-IR spectra showed the strong peaks of CHX in Ti-mSBF-adCHX and Ti-mSBF-CHX groups. However, after immersing in a phosphate buffered saline (PBS), CHX was rapidly released in Ti-mSBF-adCHX group, while it was slowly released in Ti-mSBF-CHX. We expect that the coating method of Ti-mSBF-CHX group could be used for protecting inflammation of titanium implant by incorporating antibacterial agent CHX into HAp layer.

본 연구에서는 타이타늄 상에 항균제 클로르헥시딘(chlorhexidine; CHX)이 함유된 수산화인회석을 코팅하고 그 특성을 규명하였다. CHX를 혼합한 개조된 생체유사용액(modified simulated body fluid; mSBF)에 타이타늄 디스크를 침적하여 Ti-mSBF-CHX 시편군을 준비하였다. CHX를 함유하지 않은 mSBF에 침적하여 코팅한 Ti-mSBF 시편군을 다시 CHX 용액에 침적하여 Ti-mSBF-adCHX 시편군을 준비하였다. Ti-mSBF 시편 표면에 나노 형태의 결정들로 구성된 구형의 클러스터들이 균일하게 코팅되었다. Ti-mSBF-CHX 시편에서는 이러한 클러스터들과 함께 리본형상의 결정들이 관찰되었으며, 이 결정들에서 높은 CHX 조성이 측정되었다. 두 시편 모두 HAp 결정구조가 지배적이었으며, ${\beta}-TCP$ (tricalcium phosphate)와 OCP (octacalcium phosphate) 결정구조가 Ti-mSBF-CHX 시편에서 관찰되었다. FT-IR 스펙트럼은 Ti-mSBF-adCHX와 Ti-mSBF-CHX 시편군에서 CHX의 피크가 강하게 관찰되었다. 그러나 인산완충식염수(phosphate buffered saline;PBS)에 침적한 후, CHX가 Ti-mSBF-CHX 시편에서는 천천히 용출된 반면, Ti-mSBF-adCHX 시편에서는 빠르게 용출되었다. 따라서 Ti-mSBF-CHX 시편은 골과 유사한 HAp 구조를 가지며 함유된 CHX가 지속적으로 방출될수 있기 때문에 향후 임플란트 시술에서 염증을 방지할 수 있는 코팅법으로 기대된다.

Keywords

Acknowledgement

Supported by : 한국연구재단