Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
권호 표지
DOI QR코드

DOI QR Code

The Antihyperlipidemic Effect of Lion's Mane Mushroom (Hericium erinaceus) in Hyperlipidemic Rats Induced by High Fat and Cholesterol Diet

고지방과 콜레스테롤 식이로 유도된 고지혈증 흰쥐에서 노루궁뎅이버섯의 항고지혈증 효과

  • Jang, Hyung Seok (Department of Clinical Laboratory Science, Ansan University) ;
  • Yoon, Ki Nam (Department of Clinical Laboratory Science, Ansan University)
  • 장형석 (안산대학교 임상병리과) ;
  • 윤기남 (안산대학교 임상병리과)
  • Received : 2017.07.31
  • Accepted : 2017.08.14
  • Published : 2017.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the dietary supplementation of fruiting body of Hericium erinaceus (HE) mushroom on lipid profiles of serum and histological changes of the liver in rats with high fat and cholesterol diet. Five-week old female Sprague-Dawley albino rats were divided into three groups of 8 rats each: The normal control diet (NC group), high fat and cholesterol diet (HFC group), and HFC diet supplemented with 5% fruiting powder of Hericium erinaceus (HFC+HE group). In the HFC+HE group, serum total cholesterol, low density lipoprotein, and triglyceride concentrations were significantly reduced compared with the NC group. Body weight gain of those in the HFC+HE group were lower than those in the HFC group; whereas HFC+HE had no effect on the levels of plasma albumin, creatinine, blood urea nitrogen, uric acid, glucose, and total protein. The enzyme activities related to the liver function, such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), and, alkaline phosphatase (ALP), were lower in the NC group than in the HFC group, but without significance. Feeding the mushroom increased the excretion of total lipid and cholesterol. A histopathological analysis showed that the those in the HFC group developed hepatic steatosis, whereas those in the HFC+HE group developed small fat droplet. In conclusion, these results suggest that 5% HE supplementation to HFC diet provided health benefits by acting on lowering atherogenic lipid profile in rats with high fat and cholesterol diet.

노루궁뎅이버섯의 자실체가 고지방과 콜레스테롤을 급여한 Sprague Dawley계 암컷 흰쥐의 지질대사와 생리활성에 미치는 영향을 조사하고자 생후 5주령의 흰쥐에 표준 식이를 급여한 정상군(NC군), 표준 식이에 15%의 돈지와 1%의 콜레스테롤을 첨가한 식이를 급여한 고지방 콜레스테롤군(HFC군), 고지방 콜레스테롤 식이에 노루궁뎅이버섯 자실체 분말을 5% 첨가한 군(HFC+HE군) 등 총 3개의 군으로 나누어 6주간 실험을 진행하였다. 실험동물의 체중증가와 식이효율은 HFC군에 비해 HFC+HE군이 유의하게 감소하여 NC군과 유사한 수준을 나타냈다. 식이섭취량은 NC군과 HFC군에 비해 HFC+HE군이 유의하게 낮았다. HFC+HE군의 혈청 총콜레스테롤, LDL-콜레스테롤의 농도는 NC군과 HFC군에 비해 유의하게 낮았고 동맥경화지수(AI)도 NC군과 HFC군에 비해 유의성 있게 낮았다. 그러나 혈청의 중성지방 농도는 NC군에 비해 유의성은 없었으나 HFC군에 비해 유의하게 낮았다. HFC+HE의 혈청 알부민, 크레아티닌, 요산 및 총단백질의 농도는 NC군의 수준을 나타냈다. 혈청의 AST, ALT 및 ALP 활성은 NC군과 유사하였으나 HFC군에 비해 유의하게 낮았다. 실험 5~6주에 배출된 변을 채취하여 총지질과 총콜레스테롤의 양을 분석한 결과 NC군과 HFC군에 비해 변의 총지질과 총콜레스테롤 함량이 유의하게 높아 변으로 배출된 총지질과 총콜레스테롤의 양이 많게 증가한 것으로 나타났다. 따라서 고지방 콜레스테롤식이에 5%의 노루궁뎅이버섯 자실체 분말을 첨가한 식이를 급여한 흰쥐의 체중은 정상군의 수준을 유지하였고, 혈청의 총콜레스테롤, LDL-콜레스테롤 및 중성지질의 농도도 정상군과 유사하게 낮았으며, 동맥경화지수도 낮아 노루궁뎅이버섯이 흰쥐의 지질대사를 정상으로 유지시키는 효과를 나타내는 것으로 사료되었다.

Keywords

References

  1. Moon SJ. Nutrition problems of Koreans. Korean J Nutr. 1996; 29(4):371-380.
  2. Jang SJ, Park YJ. Effects of dietary fiber sources and levels on lipid metabolism in rats fed high lard diet. Korean J Nutr. 1995;28(2):107-114.
  3. Spady DK, Woollett LA, Dietschy JM. Regulation of plasma LDL-cholesterol levels by dietary cholesterol and fatty acids. Annu Rev Nutr. 1993;13(1):355-381. https://doi.org/10.1146/annurev.nu.13.070193.002035
  4. Yao F, MacKenzie RG. Obesity drug update: The lost decade? Pharmaceut. 2010;3(12):3494-3521. https://doi.org/10.3390/ph3123494
  5. Molitoris HP. Mushrooms in medicine. Folia Microbiol 1994; 39(2):91-98. https://doi.org/10.1007/BF02906801
  6. Wasser SP. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol. 2002;60(3):258-274. https://doi.org/10.1007/s00253-002-1076-7
  7. Yoon KN, Jang HS, Jin GH. Antioxidant, anti-diabetic, anti-cholinesterase, and nitric oxide inhibitory activities of fruiting bodies of Agaricus brasiliensis. Korean J Clin Lab Sci. 2015; 47(4):194-202. https://doi.org/10.15324/kjcls.2015.47.4.194
  8. Yoon KN, Jang HS. Antioxidant and antimicrobial activities of fruiting bodies of Phellinus gilvus collected in Korea. Korean J Clin Lab Sci. 2016;48(4):355-364. https://doi.org/10.15324/kjcls.2016.48.4.355
  9. Ahn DK. Medicinal fungi in Korea. Korean J Mycol. 1992; 20(2):154-166.
  10. Park, WH. Lee, HD. Illustrated book of Korean medicinal mushrooms. Seoul: KyoHak; 2003. p442-443.
  11. Ying JZ, Mao XL, et al. Icones of medicinal fungi. Beijing: Science Press; 1987. p90-91.
  12. Kim DH, Park SR, Debnath T, Hasnat A, Pervin M, Lim BO. Evaluation of the antioxidant activity and anti-inflammatory effect of Hericium erinaceus water extracts. Korean J Medicinal Crop Sci. 2013;21(2):112-117. https://doi.org/10.7783/KJMCS.2013.21.2.112
  13. Mizuno T, Wasa T, Ito H, Suzuki C, Ukai N. Antitumor active polysaccharides isolated from the fruiting body of Hericium erinaceum, an edible and medicinal mushroom called yamabushitake or houtou. Biosci Biotechnol Biochem. 1992;56(2):347-348. https://doi.org/10.1271/bbb.56.347
  14. Kawagishi H, Shimada A, Hosokawa S, Mori H, Sakamoto H, Ishiguro Y, et al. Erinacines E, F and G stimulators of nerve growth factor synthesis from the mycelia of Hericium erinaceum. Tetrahedron Lett. 1996;37(41):7399-7402. https://doi.org/10.1016/0040-4039(96)01687-5
  15. Reeves PG, Nielsen FH, Fahey GC Jr. AIN-93 purified diets for laboratory rodents: final report of the American institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993;123(11):1939-1951. https://doi.org/10.1093/jn/123.11.1939
  16. Alam N, Yoon KN, Lee TS, Lee UY. Hypolipidemic activities of dietary Pleurotus ostreatus in hypercholesterolemic rats. Mycobiol. 2011;39(1):45-51. https://doi.org/10.4489/MYCO.2011.39.1.045
  17. Haglund O, Loustarinen R, Wallin R, Wibell I, Saldeen T. The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in humans supplemented with vitamin E. Eur J Nutr. 1991;121(2):165-169. https://doi.org/10.1093/jn/121.2.165
  18. Kobayashi H, Matsushita M, Oda K, Nishikimi N, Sakuri T, Komori K. Effects of atherosclerotic plaque on the enlargement of an experimental model of abdominal aortic aneurysm in rabbits. Eur J Vasc Endovasc Surg. 2004;28(1):71-78. https://doi.org/10.1016/j.ejvs.2004.03.015
  19. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 1957;226(1):497-509.
  20. Oh SW, Lee CU, Koh JB. Effects of Agaricus blazei Murill on lipid metabolism in rats fed high fat diet. J Korean Soc Food Sci Nutr. 2004;33(5):821-826. https://doi.org/10.3746/jkfn.2004.33.5.821
  21. Koh JB, Choi MA. The effects of Cordyceps militaris on lipid metabolism in rats fed cholesterol diet. Korean J Nutr. 2001;34(3):265-270.
  22. Park SY, Bae IY, Lee S, Lee HG. Physicochemical and hypocholesterolemic characterization of oxidized oat beta glucan. J Agric Food Chem. 2009;57(2):439-443. https://doi.org/10.1021/jf802811b
  23. Bisen PS, Baghel RK, Sanodiya BS, Thakur GS, Prasad GB. Lentinus edodes : a macrofungus with pharmacological activities. Curr Med Chem. 2010;17(22):2419-2430. https://doi.org/10.2174/092986710791698495
  24. Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature. 1993;362(6423):801-809. https://doi.org/10.1038/362801a0
  25. Koh JB. The effects of Cordyceps militaris on lipid metabolism, protein levels and enzyme activities in rats fed a high fat diet. Korean J Nutr. 2002;35(4):414-420.
  26. Bobek P, Ginter E, Kuniak L, Babala J, Jurcovicova M, Ozdin L, Cerven J. Effect of mushroom Pleurotus ostreatus and isolated fungal polysaccharide on serum and liver lipids in Syrian hamsters with hyperlipoproteinemia. Nutr. 1991;7(2):105-108.
  27. Koh JB, Choi MA. Effect of Pacilomyces japonica on lipid metabolism in rats fed high fat diet. J Korean Soc Food Sci Nutr. 2003;32(2):238-243. https://doi.org/10.3746/jkfn.2003.32.2.238
  28. Cheung PCK. The hypercholesterolemic effect of two edible mushrooms: Auricularia auricula (tree-ear) and Tremella fuciformis (white jelly-leaf) in hypercholesterolemic rats. Nutr Res. 1996;16(10):1721-1725. https://doi.org/10.1016/0271-5317(96)00191-1
  29. Agah HM, Alipour A, Janbozorgi M, Hajihosseini R, Shaghaghi, F, Golchin N, Nouhi S. Lipid profile improvement after four group psychological interventions in combination to nutritional and physical activity instructing among overweight and obese individuals. Iran J Public Health. 2013;42(1):86-95.
  30. Qu L, Xin H, Zheng G, Su Y, Ling C. Hepatoprotective activity of the total saponin from Actinidia valvata Dunn root against carbon tetrachloride-induced liver damage in mice. Evid-Based Complement Alternat Med. 2012;2012:216061
  31. Ma X, Li Z. Pathogenesis of nonalcoholic steatohepatitis (NASH). Chin J Dig Dis. 2006;7(1):7-11. https://doi.org/10.1111/j.1443-9573.2006.00237.x
  32. Zou Y, Li J, Lu C, Wang J, Ge j, Huang Y, et al. High-fat emulsion induced rat model of nonalcoholic steatohepatitis. Life Sci. 2006;79(11):1100-1107. https://doi.org/10.1016/j.lfs.2006.03.021
  33. Kim JH, Son IS, Kim JS, Kim KH, Kwon CS. Lipase-inhibitory and anti-oxidative activity of the methanol extract and the powder of Phellinus linteus. J Korean Soc Food Sci Nutr. 2008;37(2):154-161. https://doi.org/10.3746/jkfn.2008.37.2.154
  34. Yoon KN, Alam N, Shim MJ, Lee TS. Hypolipidemic and antiatherogenesis effect of culinary-medicinal pink oyster mushroom, Pleurotus salmoneostramineus L. Vass. (Higher Basidiomycetes), in hypercholesterolemic rats. Int J Med Mushrooms. 2012;14(1):27-36. https://doi.org/10.1615/IntJMedMushr.v14.i1.30
  35. Kim GY, Jung HW, Kim EJ. Anti-hyperlipidemic effect of shiitake mushroom extract in hyperlipidemic rats induced by poloxamer-407. Korean J Orient Physiol Pathol. 2013;27(4); 409-415.