DOI QR코드

DOI QR Code

한국인 장관에서 분리한 유산균의 항균활성 및 프로바이오틱스 특성 연구

Antibacterial Activity and Probiotic Properties of Lactic Acid Bacteria from Korean Intestine Origin

  • 투고 : 2017.05.10
  • 심사 : 2017.06.20
  • 발행 : 2017.06.30

초록

The purpose of this study was to investigate the probiotic properties of lactic acid bacterial strains isolated from human feces. The properties were tested on the basis of guideline for probiotic selection protocol such as tolerance for acid or bile salt, autoaggregation, antibiotic resistance, and antimicrobial activity. Total 25 lactic acid bacteria were isolated from human feces, and their antibacterial activity was tested against Staphylococcus aureus, Escherichia coli, E. coli O157:H7, Vibrio parahaemolyticus, V. alginolyticus using an agar diffusion assay. Among them, 4 selected strains were identified by analysis of their 16S rRNA, as Lactobacillus rhamnosus MG316, L. acidophilus MG501, L. reuteri MG505, and L. gasseri MG570. Results show that resistance to low pH and bile salts. Also, the selected strains were resistant to bile acid up to 3% and their autoaggregation rates were as high as 60%. All strains tested were resistance to nalidixic acid and kanamycin.

키워드

참고문헌

  1. Food and Agricultural Organization of the United Nations and World Health Organization. (2001) Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. World Health Organization.
  2. Alander, M., R. Satokari, R. Korpela, M. Saxelin, T. Vilpponen-Salmela, and A. von Wright (1999) Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption. Appl. Environ. Microbiol. 65: 351-354.
  3. Axelsson, L. T., T. C. Chung, W. G. Dobrogosz, and S. E. Lindgren (1989) Production of a broad spectrum antimicrobial substance by Lactobacillus reuteri. Microb. Ecol. Health Dis. 2: 131-136. https://doi.org/10.3109/08910608909140210
  4. Seo J. G., G. S. Lee, J. E. Kim, and M. J. Chung (2010) Development of probiotic products and challengers. KSBB J. 25: 303-310.
  5. Vrese, M., A. Stegelmann, B. Ritcher, S. Fenselau, C. Laue, and J. Schrezenmeir (2001) Probiotics: Compensation for lactase insufficiency. Am. J. Clin. Nutr. 73: S421-S429. https://doi.org/10.1093/ajcn/73.2.421s
  6. Fukushima, Y., Y. Kawata, H. Hara, A. Terada, and T. Mitsuoka (1998) Effect of a probiotic formula on intestinal immunoglobulin a production in healthy children. Int. J. Food Microbiol. 30: 39-44.
  7. Isolauri, E., Y. Sutas, P. Kankaanpaa, H. Arvilommi, and S. Salminen (2001) Probiotics: Effects on immunity. Am. J. Clin. Nutr. 73: 444-450. https://doi.org/10.1093/ajcn/73.2.444s
  8. Isolauri, E., S. Salminen, and A. C. Ouwehand (2004) Microbialgut interactions in health and disease. Probiotics. Best Prac. Res. Cl. Em. 18: 299-313. https://doi.org/10.1016/j.bpg.2003.10.006
  9. Vrese, M., A. Stegelmann, B. Ritcher, S. Fenselau, C. Laue, and J. Schrezenmeir (2001) Probiotics: compensation for lactase insufficiency. Am. J. Clin. Nutr. 73: 421?429. https://doi.org/10.1093/ajcn/73.2.421s
  10. Guo, X. H., J. M. Kim, H. M. Nam, S. Y. Park and J. M. Kim (2010) Screening lactic acid bacteria from swine origins for multistrain probiotics based on in vitro functional properties. Anaerobe 16: 321?326. https://doi.org/10.1016/j.anaerobe.2010.03.006
  11. Kassa, I. A., M. Hamze, D. Hober, N. E. Chihib, and D. Drider (2014) Identification of vaginal lactobacilli with potential probiotic propertied isolated from women in North Lebanon. Microb. Ecol. 67: 722-734. https://doi.org/10.1007/s00248-014-0384-7
  12. CLSI. (2006) Methods for Antimicrobial Dilution and Disk Susceptibility Testing ofInfrequently Isolated or Fastidious Bacteria; Approved Guideline M45-A.Clinical and Laboratory Standards Institute, Waune, PA.
  13. Tagg, J. R., A. S. Dajani, and L. W. Wannamaker (1976) Bacteriocins of gram-positive bacteria. Bacteriol. Rev. 40: 722-756.
  14. Adesokan, I. A., B. B. Odetoyinbo, and A. O. Olubamiwa (2008) Biopreservative activity of lactic acid bacteria on suya produced from poultry meat. Afr. J. Biotech. 7: 3799-3803.
  15. Obadina, A. O., O. B. Oyewole, L. O. Sanni, and K. I. Tomlins (2006) Biopreservative activities of Lactobacillus plantarum strains in fermenting cassava 'fufu'. Afr. J. Biotech. 5: 620-623.
  16. Puttalingamma, V., K. Begum, and A. S. Bawa (2006) Antimicrobial peptides-new weapons against enteric pathogens. Pak. J. Nutr. 5: 432-435. https://doi.org/10.3923/pjn.2006.432.435
  17. Bang, J. H., H. J. Shin, H. J. Choi, D. W. Kim, C. S. Ahn, Y. K. Jeong, and W. H. Joo (2012) Probiotic potential of Lactobacillus isolates. J. Life Sci. 22: 251-258. https://doi.org/10.5352/JLS.2012.22.2.251
  18. Sim, J. H., S. J. Oh, S. K. Kim, and Y. J. Baek (1995) Comparative tests on the acid tolerance of some lactic acid bacteria species isolated from lactic fermented products. Korean J. Food Sci. Technol. 27: 101-104.
  19. Saarela, M., G. Mogensen, R. Fondn, J. Mtt, and T. Mattila-Sandholm (2000) Probiotic bacteria: Safety, functional and technological properties. J. Biotechnol. 84: 197-215. https://doi.org/10.1016/S0168-1656(00)00375-8
  20. Dunne, C., L. O'Mahony, L. Murphy, G. Thornton, D. Morrissey, S. O'Halloran, M. Feeney, S. Flynn, G. Fitzgerald, C. Daly, B. Kiely, G. C. O'Sullivan, F. Shanahan, and J. K. Collins (2001) In vitro selection criteria for probiotic bacteria of human origin: Correlation with in vivo findings. Am. J. Clin. Nutr. 73: 386-392. https://doi.org/10.1093/ajcn/73.2.386s
  21. Hood, S. K., E. A. Zottola (1988) Effect of low pH on the ability of Lactobacillus acidophilus to survive and adhere to human intestinal cells. J. Food Sci 53: 1514-1520 https://doi.org/10.1111/j.1365-2621.1988.tb09312.x
  22. Mishra, V., and D. N. Prasad (2005) Application of in vitro methods for selection of Lactobacillus casei strains as potential probiotics. Int. J. Food Microbiol. 103: 109-115. https://doi.org/10.1016/j.ijfoodmicro.2004.10.047
  23. Gilliland, S. E., T. E. Staley, and L. J. Bush (1984) Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J. Dairy Sci. 67: 3045-5301. https://doi.org/10.3168/jds.S0022-0302(84)81670-7
  24. Lin, W. H., C. F. Hwangb, L. W. Chenc, and H. Y. Tsen (2006) Viable counts, characteristic evaluation for commercial lactic acid bacteria products. Food Microbiol. 23: 74-81. https://doi.org/10.1016/j.fm.2005.01.013
  25. Gilliland, S. E. and D. K. Walker (1990) Factors to consider when selecting a culture to produce of Lactobacillus acidophilus as a dietary adjunct to produce a hypocholesterolemic effect in humans. J. Dairy Sci. 73: 905-911. https://doi.org/10.3168/jds.S0022-0302(90)78747-4
  26. Del Re, B., B. Sgorbati, M. Moglioli, and D. Palenzona (2000) Adhesion, autoaggregation and hydrophobicity of 13 strains of bafidobacterium longum. Lett. Appl. Microbiol. 31:438-442. https://doi.org/10.1046/j.1365-2672.2000.00845.x
  27. Kos, B., J. Suskoviae, S. Vukoviae, M. Simpraga, J. Frece, and S. Matosiae (2003) Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J. Appl. Microbiol. 94: 981-987. https://doi.org/10.1046/j.1365-2672.2003.01915.x
  28. Malik, S., M. I. Petrova, I. J. J. Claes, T. L. A. Verhoeven, P. Busschaert, M. Vaneechoutte, B. Lievens, I. Lambrichts, R. J. Siezen, J. Balzarini, J. Vanderleyden, and S. Lebeer (2013) The highly autoaggregative and adhesive phenotype of the vaginal Lactobacillus plantarum strain CMPG5300 is sortase dependent. Appl. Environ. Microbiol. 79: 4576-4585. https://doi.org/10.1128/AEM.00926-13
  29. Franz, C. M. A. P., M. Huch, H. Abriouel, W. Holzapfel, and A. Galvez (2011) Enterococci as probiotics and their implications in food safety. Int. J. Food Microbiol. 151: 125-140. https://doi.org/10.1016/j.ijfoodmicro.2011.08.014
  30. Ogier, J. C. and P. Serror (2008) Safety assessment of dairy microorganisms: The Enterococcus genus. Int. J. Food Microbiol. 126: 291-301. https://doi.org/10.1016/j.ijfoodmicro.2007.08.017
  31. Mathur, S. and R. Singh (2005) Antibiotic resistance in food lactic acid bacteria - A review. Int. J. Food Microbiol. 105: 281-295. https://doi.org/10.1016/j.ijfoodmicro.2005.03.008

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