DOI QR코드

DOI QR Code

Effect of γ-oryzanol on Proliferation and Apoptosis of AGS Human Gastric Carcinoma Cell

감마 오리자놀의 위암세포증식억제 및 세포사멸 유도 효능

  • Received : 2017.02.02
  • Accepted : 2017.04.04
  • Published : 2017.06.30

Abstract

Gamma (${\gamma}$)-oryzanol is a substance abundant in rice, which is widely cultivated in Asian countries. In this study, we evaluated the effect of ${\gamma}$-oryzanol treatment on proliferation and apoptosis of AGS human gastric carcinoma cells. AGS cells were treated with ${\gamma}$-oryzanol for 72 h in a dose dependent manner. Treatment of ${\gamma}$-oryzanol (50, 100, and $200{\mu}g/mL$) resulted in decreased AGS cell proliferation and increased number of cells in the sub-G1 population. Additionally, apoptotic cells were investigated by annexin V staining and mitochondrial membrane potential assays. Our results indicated that ${\gamma}$-oryzanol treatment increased the number of annexin V-positive cells and depolarized cells. This demonstrated that ${\gamma}$-oryzanol is effective for the induction of apoptosis in AGS cells. We next examined the expression of promising anticancer drug target molecules, including PTEN and HSP90. We found that treatment of ${\gamma}$-oryzanol induced the expression of PTEN in AGS cells. Under the same treatment conditions, ${\gamma}$-oryzanol reduced the expression of HSP90 in AGS cells. These results suggest that ${\gamma}$-oryzanol-induced apoptosis was accompanied by changes in regulation of PTEN and HSP90 in AGS cells. Taken together, ${\gamma}$-oryzanol could be used as a functional substance for the prevention of gastric cancer.

Keywords

Acknowledgement

Supported by : 한국식품연구원

References

  1. Mortality Data. Statistics Korea. http://www.kostat.go.kr. (2015).
  2. Jung, K. W., Y. J. Won, H. J. Kong, C. M. Oh, H. Cho, D. H. Lee, and K. H. Lee (2015) Cancer statistics in Korea: incidence, mortality, survival, and prevalence in 2012. Cancer Res. Treat. 47: 127-141 https://doi.org/10.4143/crt.2015.060
  3. Sasako, M., S. Sakuramoto, H. Katai, T. Kinoshita, H. Furukawa, T. Yamaguchi, A. Nashimoto, M. Fujii, T. Nakajima, and Y. Ohashi (2011) Five-year outcomes of a randomized phase III trial comparing adjuvant chemotherapy with S-1 versus surgery alone in stage II or III gastric cancer. J. Clin. Oncol. 29: 4387-4393. https://doi.org/10.1200/JCO.2011.36.5908
  4. Noh, S. H., S. R. Park, H. K. Yang, H. C. Chung, I. J. Chung, S. W. Kim, H. H. Kim, J. H. Choi, H. K. Kim, W. Yu, J. L. Lee, D. B. Shin, J. Ji, J. S. Chen, Y. Lim, S. Ha, and Y. J. Bang (2014) CLASSIC trial investigators. Adjuvant capecitabine plus oxaliplatin for gastric cancer after D2 gastrectomy (CLASSIC): 5-year follow-up of an open-label, randomised phase 3 trial. Lancet Oncol. 15: 1389-1396. https://doi.org/10.1016/S1470-2045(14)70473-5
  5. Cazzaniga, M. and B. Bonanni (2012) Breast cancer chemoprevention: Old and new approaches. J. Biomed. Biotechnol. 2012: 985620.
  6. Gan, R.Y., H. B. Li, Z. Q. Sui, and H. Corke (2016) Absorption, metabolism, anti-cancer effect and molecular targets of epigallocatechin gallate (EGCG): An updated review. Crit. Rev. Food Sci. Nutr. in press.
  7. Yang, C., W. Du, and D. Yang (2016) Inhibition of green tea polyphenol EGCG(()-epigallocatechin-3-gallate) on the proliferation of gastric cancer cells by suppressing canonical wnt/b-catenin signalling pathway. Int. J. Food Sci. Nutr. 67: 818-827. https://doi.org/10.1080/09637486.2016.1198892
  8. Onoda, C., K. Kuribayashi, S. Nirasawa, N. Tsuji, M. Tanaka, D. Kobayashi, and N. Watanabe (2011) (-)-Epigallocatechin-3-gallate induces apoptosis in gastric cancer cell lines by down-regulating survivin expression. Int. J. Oncol. 38: 1403-1408.
  9. Liu, Y. L., G. Q. Zhang, Y. Yang, C. Y. Zhang, R. X. Fu, and Y. M. Yang (2013) Genistein induces G2/M arrest in gastric cancer cells by increasing the tumor suppressor PTEN expression. Nutr. Cancer 65: 1034-1041. https://doi.org/10.1080/01635581.2013.810290
  10. Leslie, N. R., N. Kriplani, M. A. Hermida, V. Alvarez-Garcia, and H. M. Wise (2016) The PTEN protein: Cellular localization and post-translational regulation. Biochem. Soc. Trans. 44: 273-278. https://doi.org/10.1042/BST20150224
  11. Wu, Y. and F. T. Liu (2013) Targeting mTOR: Evaluating the therapeutic potential of resveratrol for cancer treatment. Anticancer Agents Med. Chem. 13: 1032-1038. https://doi.org/10.2174/18715206113139990113
  12. Wu, J., T. Liu, Z. Rios, Q. Mei, X. Lin, and S. Cao (2016) Heat Shock Proteins and Cancer. Trends Pharmacol. Sci. in press.
  13. Henderson, A. J., C. A. Ollila, A. Kumar, E. C. Borresen, K. Raina, R. Agarwal, and E. P. Ryan (2012) Chemopreventive properties of dietary rice bran: Current status and future prospects. Adv. Nutr. 3: 643-653. https://doi.org/10.3945/an.112.002303
  14. Kim, W. H., J. H. Shin, M. K. Lee, S. H. Lee, H. H. Jang, H. S. Cho, J. T. Lee, W. T. Jeon, and J. B. Kim (2015) Treatment Effect of Green Manure Crops on Content of $\gamma$-Oryzanols from Korean Rice Variety, Unkwangbyeo. Korean J. Environ. Agric. 34: 98-104. https://doi.org/10.5338/KJEA.2015.34.2.20
  15. Bergman, C. J. and Z. Xu (2003) Genotype and environment effects on tocopherols, tocotrienols and gamma-oryzanol contents of Southern US rice. Cereal Chem. 80: 446-449. https://doi.org/10.1094/CCHEM.2003.80.4.446
  16. Goufo, P. and H. Trindade (2014) Rice antioxidants: Phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, $\gamma$-oryzanol, and phytic acid. Food Sci. Nutr. 2: 75-104. https://doi.org/10.1002/fsn3.86
  17. Islam, M. S., R. Nagasaka, K. Ohara, T. Hosoya, H. Ozaki, H. Ushio, and M. Hori (2011) Biological abilities of rice bran-derived antioxidant phytochemicals for medical therapy. Curr. Top. Med. Chem. 11: 1847-1853. https://doi.org/10.2174/156802611796235099
  18. Hirsch, G. E., M. M. Parisi, L. A. Martins, C. M. Andrade, F. M. Barbe-Tuana, and F. T. Guma (2015) $\gamma$-Oryzanol reduces caveolin-1 and PCGEM1 expression, markers of aggressiveness in prostate cancer cell lines. Prostate 75: 783-797. https://doi.org/10.1002/pros.22960
  19. Kim, S. P., M. Y. Kang, and S. H. Nam (2012) Friedman M.Dietary rice bran component $\gamma$-oryzanol inhibits tumor growth in tumorbearing mice. Mol. Nutr. Food Res. 56: 935-944. https://doi.org/10.1002/mnfr.201200057
  20. Berger, A., D. Rein, A. Schäfer, I. Monnard, G. Gremaud, P. Lambelet, and C. Bertoli (2005) Similar cholesterol-lowering properties of rice bran oil, with varied g-oryzanol, in mildly hyper cholesterolemic men. Eur. J. Nutr. 44: 163-173. https://doi.org/10.1007/s00394-004-0508-9
  21. Kong, S. H., Y. M. Choi, Y. W. Kim, D. J. Kim, and J. S. Lee (2009) Antioxidant activity and antioxidant components in methanolic extract from Geumjong rice. J. Korean Soc. Food Sci. Nutr. 38: 807-811. https://doi.org/10.3746/jkfn.2009.38.6.807
  22. Kim, D. J., S. K. Oh, M. R. Yoon, A. R. Chun, H. C. Hong, J. S. Lee, and Y. K. Kim (2010) Antioxidant compounds and antioxidant activities of the 70% ethanol extracts from brown and milled rice by cultivar. J. Korean Soc. Food Sci. Nutr. 39: 467-473. https://doi.org/10.3746/jkfn.2010.39.3.467
  23. Ohara, K., A. Uchida, R. Nagasaka, H. Ushio, and T. Ohshima (2009) The effects of hydroxycinnamic acid derivatives on adiponectin secretion. Phytomedicine 16: 130-137. https://doi.org/10.1016/j.phymed.2008.09.012
  24. Shafie, N. H., N. Mohd Esa, H. Ithnin H, A. Md Akim, N. Saad, and A. K. Pandurangan (2013) Preventive inositol hexaphosphate extracted from rice bran inhibits colorectal cancer through involvement of Wnt/$\beta$-catenin and COX-2 pathways. Biomed. Res. Int. 2013: 681027.
  25. Graves, A. M., N. Hettiarachchy, S. Rayaprolu, R. Li, R. Horax, and H. S. Seo (2016) Bioactivity of a rice bran-derived peptide and its sensory evaluation and storage stability in orange juice. J. Food Sci. 81: H1010-5. https://doi.org/10.1111/1750-3841.13245
  26. Orditura, M., G. Galizia, V. Sforza, V. Gambardella, A. Fabozzi, M. M. Laterza, F. Andreozzi, J. Ventriglia, B. Savastano, A. Mabilia, E. Lieto, F. Ciardiello, and F. De Vita (2014) Treatment of gastric cancer. World J. Gastroenterol. 20: 1635-1649. https://doi.org/10.3748/wjg.v20.i7.1635
  27. Farsinejad, S., Z. Gheisary, S. Ebrahimi Samani, and A. M. Alizadeh (2015) Mitochondrial targeted peptides for cancer therapy. Tumour Biol. 36: 5715-5725. https://doi.org/10.1007/s13277-015-3719-1
  28. Khan, K. H., T. A. Yap, L. Yan, and D. Cunningham (2013) Targeting the PI3K-AKT-mTOR signaling network in cancer. Chin. J. Cancer. 32: 253-265. https://doi.org/10.5732/cjc.013.10057
  29. Soga, S., S. Akinaga, and Y. Shiotsu Y (2013) Hsp90 inhibitors as anti-cancer agents, from basic discoveries to clinical development. Curr. Pharm. Des. 19: 366-376. https://doi.org/10.2174/138161213804143617
  30. Bao, X. H., M. Takaoka, H. F. Hao, T. Fukazawa, T. Yamatsuji, K. Sakurama, N. Takigawa, M. Nakajima, T. Fujiwara, and Y. Naomoto (2013) Antiproliferative effect of the HSP90 inhibitor NVP-AUY922 is determined by the expression of PTEN in esophageal cancer. Oncol. Rep. 29: 45-50. https://doi.org/10.3892/or.2012.2074

Cited by

  1. 오미자 박 추출물 및 schizandrin에 의한 암세포 항성장 및 세포사멸 활성 vol.28, pp.4, 2018, https://doi.org/10.5352/jls.2018.28.4.415