Journal of Biosystems Engineering

Korean Society for Agricultural Machinery (한국농업기계학회)
권호 표지
DOI QR코드

DOI QR Code

Phenotyping of Low-Temperature Stressed Pepper Seedlings Using Infrared Thermography

  • Park, Eunsoo (Department of Biosystems Machinery Engineering, Chungnam National University) ;
  • Hong, Suk-Ju (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Lee, Ah-Yeong (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Park, Jongmin (Department of Bio-industrial Machinery Engineering, Pusan National University) ;
  • Cho, Byoung-Kwan (Department of Biosystems Machinery Engineering, Chungnam National University) ;
  • Kim, Ghiseok (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University)
  • Received : 2017.06.02
  • Accepted : 2017.08.14
  • Published : 2017.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: This study was performed to evaluate the feasibility of using an infrared thermography technique for phenotype analysis of pepper seedlings exposed to a low-temperature environment. Methods: We employed an active thermography technique to evaluate the thermal response of pepper seedlings exposed to low-temperature stress. The temperatures of pepper leaves grown in low-temperature conditions ($5^{\circ}C$, relative humidity [RH] 50%) for four periods (6, 12, 24, and 48 h) were measured in the experimental setting ($23^{\circ}C$, RH 70%) as soon as pepper seedling samples were taken out from the low-temperature environment. We also assessed the visible images of pepper seedling samples that were exposed to low-temperature stress to estimate appearance changes. Results: The greatest appearance change was observed for the low-temperature stressed pepper seedlings that were exposed for 12 h, and the temperature from these pepper seedling leaves was the highest among all samples. In addition, the thermal image of low-temperature stressed pepper seedlings for 6 h exhibited the lowest temperature. Conclusions: We demonstrated that the leaf withering owing to the water deficiency that occurred under low-temperature conditions could induce an increase in temperature in plant leaves using the infrared thermography technique. These results suggested that the time-resolved and averaged thermal signals or temperatures of plants could be significantly associated with the physiological or biochemical characteristics of plants exposed to low-temperature stress.

Keywords

References

  1. Badea, S and S. K. Basu. 2009. The effect of low temperature on metabolism of membrane lipids in plants and associated gene expression. Plant Omics Journal 2(2):78-84.
  2. Ballester, C., J. Castel, M. A. Jimenez-Bello, J. R Castel and D. S. Intrigliolo. 2013. Thermographic measurement of canopy temperature is a useful tool for predicting water deficit effects on fruit weight in citrus trees. Agricultural Water Management 122:1-6. https://doi.org/10.1016/j.agwat.2013.02.005
  3. Baranowski, P., W. Mazurek and R. T. Walczak. 2003. The use of thermography for pre-sowing evaluation of seed germination capacity. ISHS Acta Horticulturae 604: International Conference on Quality in Chains. An Integrated View on Fruit and Vegetable Quality 459-465.
  4. Berger, B., B. Parent and M. Tester. 2010. High-throughput shoot imaging to study drought responses. Journal of Experimental Botany 61(13):3519-3528. https://doi.org/10.1093/jxb/erq201
  5. Bocka, C. H., G. H. Pooleb, P. E Parkerc and T. R. Gottwaldb. 2010. Plant disease severity estimated visually, by digital photography and image analysis, and by hyperspectral imaging. Critical Reviews in Plant Sciences 29(2):59-107. https://doi.org/10.1080/07352681003617285
  6. David, H., R. G Diddahally and O. Stig. 2010. Phenomics: the next challenge. Nature Reviews Genetics 11:855-866. https://doi.org/10.1038/nrg2897
  7. De Palma, M., S. Grillo, I. Massarelli, A. Costa, G. Balogh, L. Vigh, and A. Leone. 2008. Regulation of desaturase gene expression, changes in membrane lipid composition and freezing tolerance in potato plants. Molecular Breeding 21(1):15-26. https://doi.org/10.1007/s11032-007-9105-y
  8. Furbank, R. T and M. Tester. 2011. Phenomics - technologies to relieve the phenotyping bottleneck. Trends in Plant Science 16(13):635-644. https://doi.org/10.1016/j.tplants.2011.09.005
  9. Hildebrandt, A., C. Raschner, and K. Ammer. 2010. An overview of recent application of medical infrared thermography in sports medicine in Austria. Sensors 10(5):4700-4715. https://doi.org/10.3390/s100504700
  10. Jackson, R. D., S. B. Idso, R. J. Reginato and P. J. Pinter Jr. 1981. Canopy temperature as a crop water stress indicator. Water Resources Research 17(4):1133-1138. https://doi.org/10.1029/WR017i004p01133
  11. Kim, J. W., E. S. Park, B. K. Cho and D. S. Kim. 2013. Temperature response of rice (Oryza sativa) under saline and drought stress. Proceedings of the Korean Society of Crop Science Conference 135.
  12. Lourtie, E., M. Bonnet and L. Bosschaert. 1995. New glyphosate screening technique by infrared thermometry. Fourth International Symposium on Adjuvants for Agrochemicals, Australia 193:297-302.
  13. Maes, W. H and K. Steppe. 2012. Estimating evapotranspiration and drought stress with ground-based thermal remote sensing in agriculture: a review. Journal of Experimental Botany 63(13):4671-4712. https://doi.org/10.1093/jxb/ers165
  14. Park, E. S and B. K. Cho. 2014. Development of drought stress measurement method for red pepper leaves using hyperspectral short wave infrared imaging technique. Protected Horticulture and Plant Factory 23(1):50-55. https://doi.org/10.12791/KSBEC.2014.23.1.050
  15. Reid, M. S. 1991. Effects of low temperatures on ornamental plants. ISHS Acta Horticulturae 298: Hortifroid, V International Symposium on Postharvest Physiology of Ornamental Plants; Importance of Cold in Ornamental Horticulture 215-223.
  16. Stijn, D., W. Nathalie and I. Dirk. 2013. Cell to whole-plant phenotyping: the best is yet to come. Trends in Plant Science 18(8):428-439. https://doi.org/10.1016/j.tplants.2013.04.008
  17. Tardieu, F. 2012. Any trait or trait-related allele can confer drought tolerance:just design the right drought scenario. Journal of Experimental Botany 63(1):25-31. https://doi.org/10.1093/jxb/err269
  18. Tasseva, G., J. D. De Virville, C. Cantrel, F. Moreau and A. Zachowski. 2004. Changes in the endoplasmic reticulum lipid proprieties in response to low temperature in Brassica napus. Plant Physiology and Biochemistry 42(10):811-822. https://doi.org/10.1016/j.plaphy.2004.10.001