The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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Effect of Skin Fat Lipid Barrier Formation on Hataedock with Coptis Japonica & Glycyrrhiza Uralensis

황련감초 하태독법의 피부 지방장벽형성 증진효과

  • Ahn, Sang Hyun (Department of Anatomy, College of Korean Medicine, Semyung University) ;
  • Kim, Ki Bong (School of Korean Medicine, Pusan National University)
  • 안상현 (세명대학교 한의과대학 해부학교실) ;
  • 김기봉 (부산대학교 한의학전문대학원 한방소아과교실)
  • Received : 2017.07.12
  • Accepted : 2017.08.10
  • Published : 2017.08.31
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Abstract

Objectives This study is conducted to evaluate skin fat barrier formation of Hataedock using the Coptis japonica & Glycyrrhiza uralensis extract. Methods The 3-week-old NC/Nga mice were divided into 3 groups: control group (Ctrl), Hataedock-treated group that uses the Coptis japonica & Glycyrrhiza uralensis (CGT) extract, and Hataedock-treated group that uses Bifidobacterium (BBT). After 2 weeks, changes in immunohistochemicals, and skin-lipid-barrier regulators were observed for the effects of Hataedock. Results In CGT group, loricrin-positive reaction has been increased by 231%, along with involucrin-positive reaction by 90%, filaggrin-positive reaction by 143%, and ASM-positive reaction by 341% in the stratum corneum. Conclusions Hataedock, using the extract of Coptis japonica & Glycyrrhiza uralensis, increased the expression of proteins promoting keratinocyte differentiation. This leads into conclusion that Hataedock may increase the keratinocyte formation and function which promotes skin barrier formation.

Keywords

References

  1. Kim YJ, Ahn JY, Seo SJ, Hong CK. The effect of retinoic acid on expression of human beta defensin-2 and LL-37 in keratinocyte. J Korean Invest Dermatol. 2007;14(1):29-35.
  2. Kwon MS, Choi TB, Kim GY. The effect on the skin barrier function of ceramide. Korean J Aesthet Soc. 2005;3(1):131-7.
  3. Yoon YM, Yoon JY, Lim KM, Hahn HJ, Kim YR, Ahn KJ, An SK. Effects of the complex containing Centella asiatica - and folic acid-fermented extracts, acetyl glutamine, and nicotinic acid adenine dinucleotide phosphate on the inhibition of senescence and melanogenesis, promotion of collagen expression, cellular regeneration, and keratinocyte differentiation, and anti-inflammation. Korean J Aesthet Soc. 2013;11(4):675-84.
  4. Steinert PM, Marekov LN. The proteins elafin, filaggrin, keratin intermediate filaments, loricrin, and small proline-rich proteins 1 and 2 are isodipeptide cross-linked components of the human epidermal cornified cell envelope. J Biol Chem. 1995;270(30):17702-11. https://doi.org/10.1074/jbc.270.30.17702
  5. Steven AC, Steinert PM. Protein composition of cornified cell envelopes of epidermal keratinocytes. J Cell Sci. 1994;107(2):693-700.
  6. Elias PM, Menon GK. Structural and lipid biochemical correlates of the epidermal permeability barrier. Adv Lipid Res. 1991;24:1-26.
  7. Steinert PM, Cantieri JS, Teller DC, Lonsdale-Eccles JD, Dale BA. Characterization of a class of cationic proteins that specifically interact with intermediate filaments. Proc Natl Acad Sci USA. 1981;78(7):4097-101. https://doi.org/10.1073/pnas.78.7.4097
  8. Cornelissen C, Marquardt Y, Czaja K, Wenzel J, Frank J, Luscher-Firzlaff J, Luscher B, Baron J. IL-31 regulates differentiation and filaggrin expression in human organotypic skin models. J Allergy Clin Immunol. 2012;129(2):426-33. https://doi.org/10.1016/j.jaci.2011.10.042
  9. Irvine AD, McLean WH. Breaking the (un)sound barrier: filaggrin is a major gene for atopic dermatitis. J Invest Dermatol. 2006;126(6):1200-2. https://doi.org/10.1038/sj.jid.5700365
  10. Palmer CN, Irvine AD, Terron-Kwiatkowski A, Zhao Y, Liao H, Lee SP, Goudie DR, Sandilands A, Campbell LE, Smith FJ, O'Regan GM, Watson RM, Cecil JE, Bale SJ, Compton JG, DiGiovanna JJ, Fleckman P, Lewis-Jones S, Arseculeratne G, Sergeant A, Munro CS, Houate BE, McElreavey K, Halkjaer LB, Bisgaard H, Mukhopadhyay S, McLean WH. Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis. Nat Genet. 2006;38(4):441-6. https://doi.org/10.1038/ng1767
  11. O'Regan GM, Irvine AD. The role of filaggrin in the atopic diathesis. Clin Exp Allergy. 2010;40(7):965-72. https://doi.org/10.1111/j.1365-2222.2010.03522.x
  12. Barnes KC. An update on the genetics of atopic dermatitis: scratching the surface in 2009. J Allergy Clin Immunol. 2010;125(1):16-29. https://doi.org/10.1016/j.jaci.2009.11.008
  13. Kim BB, Kim JR, Kim JH, Kim YA, Park JS, Yeom MH, Lee HJ, Lee KW, Kang NJ. 7,3',4'-Trihydroxyisoflavone ameliorates the development of Dermatophagoides farinae-induced atopic dermatitis in NC/Nga mice. Evid Based Complement Alternat Med. 2013;2013:1-10.
  14. Kang MY, Jang GT, Kim JH. A study on fetal toxicosis removal therapy. J Pediatr Korean Med. 2003;17(1):9-51.
  15. Tang J, Feng YB, Tsao S, Wang N, Curtain R, Wang YW. Berberine and Coptidis rhizoma as novel antineoplastic agents: a review of traditional use and biomedical investigations. J Ethnopharmacol. 2009;126(1):5-17. https://doi.org/10.1016/j.jep.2009.08.009
  16. Kaboli PJ, Rahmat A, Ismail P, Ling KH. Targets and mechanisms of berberine, a natural drug with potential to treat cancer with special focus on breast cancer. Eur J Pharmacol. 2014;740:584-95. https://doi.org/10.1016/j.ejphar.2014.06.025
  17. Ortiz LM, Lombardi P, Tillhon M, Scovassi AI. Berberine, an epiphany against cancer. Molecules. 2014;19(8):12349-67. https://doi.org/10.3390/molecules190812349
  18. Yu M, Tong X, Qi B, Qu H, Dong S, Yu B. Berberine enhances chemosensitivity to irinotecan in colon cancer via inhibition of $NF-{\kappa}B$. Mol Med Rep. 2014;9(1):249-54. https://doi.org/10.3892/mmr.2013.1762
  19. Chin LW, Cheng YW, Lin SS, Lai YY, Lin LY, Chou MY, Chou MC, Yang CC. Anti-herpes simplex virus effects of berberine from Coptidis rhizoma, a major component of a Chinese herbal medicine, Ching-Wei-San. Arch Virol. 2010;155(12):1933-41. https://doi.org/10.1007/s00705-010-0779-9
  20. Yu YE, Park EY, Jung DH, Byun SH, Kim SC, Park SM. Antibacterial activity of oriental medicinal herb extracts against skin pathogens. J Life Sci. 2010;20(7):1143-50. https://doi.org/10.5352/JLS.2010.20.7.1143
  21. Miao KL, Zhang JZ, Dong Y, Xi YF. Research progress on the chemical compounds and pharmacology of Sophora flavescens. Nat Prod Res Dev. 2001;13(2):69-73.
  22. Kusano G, Shibano M, Watanabe H. Pharmaceutical botanical studies on some Glycyrrhiza species. Yakugaku Zasshi. 2003;123(8):619-31. https://doi.org/10.1248/yakushi.123.619
  23. Kim HJ, Bae JY, Jang HN, Park SN. Comparative study on antimicrobial activity of Glycyrrhiza uralensis and Glycyrrhiza glabra extracts in various country of origin as natural antiseptics. J Microbiol Biotechnol. 2013;41(3):358-66.
  24. Kim HJ, Jang HN, Bae JY, Ha JH, Park SN. Antimicrobial activity, quantification and bactericidal activities of licorice active ingredients. J Microbiol Biotechnol. 2014;42(2):386-92.
  25. Cha HY, Ahn SH, Jeong AR, Cheon JH, Park SY, Choi JY, Kim KB. Anti-inflammatory effects of Hataedock extracted from Coptidis rhizoma and Glycyrrhiza uralensis on atopic dermatitis-like skin lesions of NC/Nga mouse. J Int Korean Med. 2015;36(4):486-97.
  26. Nobaek S, Johansson ML, Molin G, Ahrne S, Jeppsson B. Alteration of intestinal microflora is associated with reduction in abdominal bloating and pain in patients with irritable bowel syndrome. Am J Gastroenterol. 2000;95(5):1231-8. https://doi.org/10.1111/j.1572-0241.2000.02015.x
  27. Forslind B. A domain mosaic model of the skin barrier. Acta Derm Venereol. 1994;74(1):1-6.
  28. Mohsenzadeh M. Evaluation of antibacterial activity of selected Iranian essential oils against Staphylococcus aureus and Escherichia coli in nutrient broth medium. Pak J Biol Sci. 2007;10(20):3693-7. https://doi.org/10.3923/pjbs.2007.3693.3697
  29. Rasul A, Akhtar N, Khan BA, Mahmood T, Uz Zaman S, Khan HM. Formulation development of a cream containing fennel extract: in vivo evaluation for anti-aging effects. Pharm. 2012;67(1):54-8.
  30. Yu HY, Yang IJ, Lincha VR, Park IS, Lee DU, Shin HM. The effects of the fruits of Foeniculum vulgare on skin barrier function and hyaluronic acid production in HaCaT keratinocytes. J Life Sci. 2015;25(8):880-8. https://doi.org/10.5352/JLS.2015.25.8.880
  31. Steinert PM, Marekov LN. The proteins elafin, filaggrin, keratin intermediate filaments, loricrin, and small proline-rich proteins 1 and 2 are isodipeptide cross-linked components of the human epidermal cornified cell envelope. J Biol Chem. 1995;270(30):17702-11. https://doi.org/10.1074/jbc.270.30.17702
  32. Kwon YB, Choi DK, Sohn KC, Jeon EK, Nam MS, Lee JH, Kim CD. Effects of colostrum on keratinocyte differentiation and wound healing. Korean J Invest Dermatol. 2007;14(2):45-50.
  33. Imokawa G, Abe A, Jin K, Higaki Y, Kawashima M, Hidano A. Decreased level of ceramides in stratum corneum of atopic dermatitis: an etiologic factor in atopic dry skin?. J Invest Dermatol. 1991;96(4):523-6. https://doi.org/10.1111/1523-1747.ep12470233
  34. Choi MJ, Maibach HI. Role of ceramides in barrier function of healthy and diseased skin. Am J Clin Dermatol. 2005;6(4):215-23. https://doi.org/10.2165/00128071-200506040-00002
  35. Hara J, Higuchi K, Okamoto R, Kawashima M, Imokawa G. High-expression of sphingomyelin deacylase is an important determinant of ceramide deficiency leading to barrier disruption in atopic dermatitis. J Invest Dermatol. 2000;115(3):406-13. https://doi.org/10.1046/j.1523-1747.2000.00072.x
  36. Murata Y, Ogata J, Higaki Y, Kawashima M, Yada Y, Higuchi K, Tsuchiya T, Kawaminami S, Imokawa G. Abnormal expression of sphingomyelin acylase in atopic dermatitis: an etiologic factor for ceramide deficiency?. J Invest Dermatol. 1996;106(6):1242-9. https://doi.org/10.1111/1523-1747.ep12348937
  37. Fluhr JW, Kao J, Jain M, Ahn SK, Feingold KR, Elias PM. Generation of free fatty acids from phospholipids regulates stratum corneum acidification and integrity. J Invest Dermatol. 2001;117(1):44-51. https://doi.org/10.1046/j.0022-202x.2001.01399.x
  38. Song JH, Ahn SH, Cheon JH, Park SY, Kim HH, Kim KB. Effects of Hataedock with Douchi on 2,4-dinitrofluorobenzene-induced atopic dermatitis-like skin lesion in NC/Nga mice. J Physiol Pathol Korean Med. 2016;30(2):109-15. https://doi.org/10.15188/kjopp.2016.04.30.2.109
  39. Hachem JP, Crumrine D, Fluhr J, Brown BE, Feingold KR, Elias PM. pH directly regulates epidermal permeability barrier homeostasis and stratum corneum integrity/cohesion. J Invest Dermatol. 2003;121(2):345-53. https://doi.org/10.1046/j.1523-1747.2003.12365.x
  40. Proksch E, Brandner JM, Jensen JM. The skin: an indispensable barrier. Exp Dermatol. 2008;17(12):1063-72. https://doi.org/10.1111/j.1600-0625.2008.00786.x
  41. Baroni A, Buommino E, De Gregorio V, Ruocco E, Ruocco V, Wolf R. Structure and function of the epidermis related to barrier properties. Clin Dermatol. 2012;30(3):257-362. https://doi.org/10.1016/j.clindermatol.2011.08.007
  42. Hachem JP, Man MQ, Crumrine D, Uchida Y, Brown BE, Rogiers V, Roseeuw D, Feingold KR, Elias PM. Sustained serine proteases activity by prolonged increase in pH leads to degradation of lipid processing enzymes and profound alterations of barrier function and stratum corneum integrity. J Invest Dermatol. 2005;125(3):510-20. https://doi.org/10.1111/j.0022-202X.2005.23838.x
  43. Hachem JP, Crumrine D, Fluhr J, Brown BE, Feingold KR, Elias PM. pH directly regulates epidermal permeability barrier homeostasis and stratum corneum integrity/cohesion. J Invest Dermatol. 2003;121(2):345-53. https://doi.org/10.1046/j.1523-1747.2003.12365.x
  44. Schmid-Wendtner MH, Korting HC. The pH of the skin surface and its impact on the barrier function. Skin Pharmacol Physiol. 2006;19(6):296-302. https://doi.org/10.1159/000094670
  45. Rippke F, Schreiner V, Doering T, Maibach HI. Stratum corneum pH in atopic dermatitis: impact on skin barrier function and colonization with Staphylococcus aureus. Am J Clin Dermatol. 2004;5(4):217-23. https://doi.org/10.2165/00128071-200405040-00002
  46. Elias PM, Ghadially R. The aged epidermal permeability barrier: basis for functional abnormalities. Clin Geriatr Med. 2002;18(1):103-20. https://doi.org/10.1016/S0749-0690(03)00037-5
  47. Steinett PM, Marekov LN. Direct evidence that involucrin is a major early isopeptide cross-linked component of the keratinocyte cornified cell envelope. J Biol Chem. 1997;272(3):2021-30. https://doi.org/10.1074/jbc.272.3.2021
  48. Kim BE, Leung DY, Boguniewicz M, Howell MD. Loricrin and involucrin expression is down-regulated by Th2 cytokines through STAT-6. Clin Immunol. 2008;126(3):332-7. https://doi.org/10.1016/j.clim.2007.11.006
  49. Smith FJ, Irvine AD, Terron-Kwiatkowski A, Sandilands A, Campbell LE, Zhao Y, Liao H, Evans AT, Goudie DR, Lewis-Jones S, Arseculeratne G, Munro CS, Sergeant A, O'Regan G, Bale SJ, Compton JG, DiGiovanna JJ, Presland RB, Fleckman P, McLean WH. Loss-of-function mutations in the gene encoding filaggrin cause ichthyosis vulgaris. Nat Genet. 2006;38(3):337-42. https://doi.org/10.1038/ng1743
  50. O'Regan GM, Irvine AD. The role of filaggrin in the atopic diathesis. Clin Exp Allergy. 2010;40(7):965-72. https://doi.org/10.1111/j.1365-2222.2010.03522.x
  51. Pullmannova P, Stankova K, Pospisilova M, Skolova B, Zbytovska J, Vavrova K. Effects of sphingomyelin/ceramide ratio on the permeability and microstructure of model stratum corneum lipid membranes. Biochim Biophys Acta. 2014;1838(8):2115-26. https://doi.org/10.1016/j.bbamem.2014.05.001
  52. Jensen JM, Folster-Holst R, Baranowsky A, Schunck M, Winoto-Morbach S, Neumann C, Schutze S, Proksch E. Impaired sphigomyelinase activity and epidermal differentiation in atopic dermatitis. J Invest Dermatol. 2004;122(6):1423-31. https://doi.org/10.1111/j.0022-202X.2004.22621.x
  53. Elias PM, Schmuth M. Abnormal skin barrier in the etiopathogenesis of atopic dermatitis. Curr Opin Allergy Clin Immunol. 2009;9(5):437-46. https://doi.org/10.1097/ACI.0b013e32832e7d36
  54. Elias PM, Steinhoff M. "Outside-to-inside" (and now back to "outside") pathogenic mechanisms in atopic dermatitis. J Invest Dermatol. 2008;128(5):1067-70. https://doi.org/10.1038/jid.2008.88
  55. Hatano Y, Terashi H, Arakawa S, Katagiri K. Interleukin-4 suppresses the enhancement of ceramide synthesis and cutaneous permeability barrier functions induced by tumor necrosis factor-alpha and interferon-gamma in human epidermis. J Invest Dermatol. 2005;124(4):786-92. https://doi.org/10.1111/j.0022-202X.2005.23651.x
  56. Albanesi C, Fairchild HR, Madonna S, Scarponi C, Pita OD, Leung DY, Howell MD. IL-4 and IL-13 negatively regulate TNF-alpha- and IFN-gamma-induced beta-defensin expression through STAT-6, suppressor of cytokine signaling (SOCS)-1, and SOCS-3. J Immunol. 2007;179(2):984-92. https://doi.org/10.4049/jimmunol.179.2.984