Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Nitrite Removal Characteristics and Application of Bosea sp. Isolated from BFT System Culture Water

BFT 시스템 사육 수에서 분리한 Bosea sp.의 아질산 제거 특성과 활용

  • Lee, Hye-Jin (Department of Molecular Biology, Dong-eui University) ;
  • Kim, Hyo-Won (Aquaculture Management Division, National Institute of Fisheries Science) ;
  • Kim, Myung-Hee (Department of Molecular Biology, Dong-eui University) ;
  • Kim, Dae-Jung (Aquaculture Management Division, National Institute of Fisheries Science) ;
  • Kim, Kwang-Hyun (Department of Life Science and Biotecnology, Dong-eui University) ;
  • Bae, Sun-Hye (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Lee, Kyu-Tae (NeoEnBiz corporation) ;
  • Han, Chang-Hee (Department of Molecular Biology, Dong-eui University)
  • 이혜진 (동의대학교 분자생물학과) ;
  • 김효원 (국립수산과학원 양식관리과) ;
  • 김명희 (동의대학교 분자생물학과) ;
  • 김대중 (국립수산과학원 양식관리과) ;
  • 김광현 (동의대학교 생명응용학과) ;
  • 배선혜 (국립수산과학원 서해수산연구소 양식산업과) ;
  • 이규태 ((주)네오엔비즈) ;
  • 한창희 (동의대학교 분자생물학과)
  • Received : 2017.05.26
  • Accepted : 2017.07.05
  • Published : 2017.08.31
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Abstract

This study was performed to isolate bacteria that could control the nitrite levels in a biofloc technology (BFT) culture tank. Nitrite-eliminating bacteria were isolated from a BFT culture tank rearing goldfish, and the isolated bacterium exhibiting the most potent nitrite eliminating ability was labeled as the "NOBSB1" strain. Sequencing the 16S rRNA revealed that NOBSB1 is a species in the genera Bosea. NOBSB1 had the following characteristics with regard to nitrite removal: (1) it removed nitrite by functioning heterotrophically in the presence of a carbon source (sugars); (2) it eliminated nitrite most effectively within a temperature range of $20-30^{\circ}C$, but its activity decreased at temperatures above $35^{\circ}C$ and below $20^{\circ}C$; (3) it had optimum nitrite removal ability within a pH range of 6.0-8.0; (4) it removed nitrite more effectively under hypoxic than aerobic conditions. NOBSB1 inoculation did not decrease ammonia or nitrate levels, but eliminated nitrite in a BFT culture tank rearing common carp (Cyprinus carpio). After inoculating the NOBSB1 strain in a BFT culture tank, NOBSB1 controlled and sufficiently reduced the nitrite concentration in the tank.

Keywords

References

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